The skin microbiome : investigations on skin malignancies and preterm newborn skin

Research output: ThesisDoctoral ThesisCollection of Articles

Abstract

The skin microbiome – Investigations on skin malignancies and preterm newborn skin Skin disorders have been associated with specific microbiome changes and raised an interest in developing new diagnostic methods and treatments. Our object was to investigate the microbiome in skin cancer (melanoma) and inflammatory skin disorders (parapsoriasis) and to explore the skin microbiome in very low birth weight infants in intensive care and possible association to neonatal sepsis. Microbiome samples were taken of 15 cutaneous melanomas and 17 benign melanocytic nevi, of 13 patients with parapsoriasis and 12 very low birth weight infants during treatment in intensive care. Sequencing was carried out on 454 GS-FLX Titanium and Illumina MiSeq platforms and the data was analyzed by bioinformatics. There were no significant differences in the microbiome of melanomas, melanocytic nevi and controls. Additionally, the microbiome showed no significant differences between parapsoriasis and the same patient’s healthy skin. We observed a high cutaneous microbial diversity in most of the infants at birth and there was a decrease in diversity during the first three weeks of life. There was no association between microbiome changes and neonatal sepsis. The results suggest that microbiome swab sampling may not be helpful in diagnostics of melanoma or parapsoriasis. Moreover, microbiome changes seem not to play a role in parapsoriasis. We could demonstrate that very low birth weight infants in intensive care have a high cutaneous microbial diversity during the first days of life regardless of the way of delivery, prematurity causes or perinatal infections. The diversity decreases during the first weeks of life possibly due to intensive care treatment and antibiotics. Neonatal sepsis is not linked to microbiome changes and it is likely that other factors play a role, e.g. skin injury by medical devices and concomitant infections.
Original languageEnglish
Supervisors/Advisors
  • Lauerma, Antti, Supervisor
Place of PublicationHelsinki
Publisher
Print ISBNs978-951-51-3156-0
Electronic ISBNs978-951-51-3157-7
Publication statusPublished - 2018
MoE publication typeG5 Doctoral dissertation (article)

Fields of Science

  • Infant, Premature
  • Skin
  • +microbiology
  • Microbiota
  • Melanoma
  • Parapsoriasis
  • Nevus, Pigmented
  • Skin Neoplasms
  • Infant, Very Low Birth Weight
  • Infant, Low Birth Weight
  • Neonatal Sepsis
  • Enterotoxins
  • Lymphoma, T-Cell
  • Mycosis Fungoides
  • Staphylococcal Infections
  • Staphylococcus aureus
  • Intensive Care, Neonatal
  • 3121 Internal medicine

Cite this

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title = "The skin microbiome : investigations on skin malignancies and preterm newborn skin",
abstract = "The skin microbiome – Investigations on skin malignancies and preterm newborn skin Skin disorders have been associated with specific microbiome changes and raised an interest in developing new diagnostic methods and treatments. Our object was to investigate the microbiome in skin cancer (melanoma) and inflammatory skin disorders (parapsoriasis) and to explore the skin microbiome in very low birth weight infants in intensive care and possible association to neonatal sepsis. Microbiome samples were taken of 15 cutaneous melanomas and 17 benign melanocytic nevi, of 13 patients with parapsoriasis and 12 very low birth weight infants during treatment in intensive care. Sequencing was carried out on 454 GS-FLX Titanium and Illumina MiSeq platforms and the data was analyzed by bioinformatics. There were no significant differences in the microbiome of melanomas, melanocytic nevi and controls. Additionally, the microbiome showed no significant differences between parapsoriasis and the same patient’s healthy skin. We observed a high cutaneous microbial diversity in most of the infants at birth and there was a decrease in diversity during the first three weeks of life. There was no association between microbiome changes and neonatal sepsis. The results suggest that microbiome swab sampling may not be helpful in diagnostics of melanoma or parapsoriasis. Moreover, microbiome changes seem not to play a role in parapsoriasis. We could demonstrate that very low birth weight infants in intensive care have a high cutaneous microbial diversity during the first days of life regardless of the way of delivery, prematurity causes or perinatal infections. The diversity decreases during the first weeks of life possibly due to intensive care treatment and antibiotics. Neonatal sepsis is not linked to microbiome changes and it is likely that other factors play a role, e.g. skin injury by medical devices and concomitant infections.",
keywords = "Infant, Premature, Skin, +microbiology, Microbiota, Melanoma, Parapsoriasis, Nevus, Pigmented, Skin Neoplasms, Infant, Very Low Birth Weight, Infant, Low Birth Weight, Neonatal Sepsis, Enterotoxins, Lymphoma, T-Cell, Mycosis Fungoides, Staphylococcal Infections, Staphylococcus aureus, Intensive Care, Neonatal, 3121 Internal medicine",
author = "Alexander Salava",
note = "M1 - 64 s. + liitteet",
year = "2018",
language = "English",
isbn = "978-951-51-3156-0",
publisher = "[A. Salava]",
address = "Finland",

}

The skin microbiome : investigations on skin malignancies and preterm newborn skin. / Salava, Alexander.

Helsinki : [A. Salava], 2018. 64 p.

Research output: ThesisDoctoral ThesisCollection of Articles

TY - THES

T1 - The skin microbiome : investigations on skin malignancies and preterm newborn skin

AU - Salava, Alexander

N1 - M1 - 64 s. + liitteet

PY - 2018

Y1 - 2018

N2 - The skin microbiome – Investigations on skin malignancies and preterm newborn skin Skin disorders have been associated with specific microbiome changes and raised an interest in developing new diagnostic methods and treatments. Our object was to investigate the microbiome in skin cancer (melanoma) and inflammatory skin disorders (parapsoriasis) and to explore the skin microbiome in very low birth weight infants in intensive care and possible association to neonatal sepsis. Microbiome samples were taken of 15 cutaneous melanomas and 17 benign melanocytic nevi, of 13 patients with parapsoriasis and 12 very low birth weight infants during treatment in intensive care. Sequencing was carried out on 454 GS-FLX Titanium and Illumina MiSeq platforms and the data was analyzed by bioinformatics. There were no significant differences in the microbiome of melanomas, melanocytic nevi and controls. Additionally, the microbiome showed no significant differences between parapsoriasis and the same patient’s healthy skin. We observed a high cutaneous microbial diversity in most of the infants at birth and there was a decrease in diversity during the first three weeks of life. There was no association between microbiome changes and neonatal sepsis. The results suggest that microbiome swab sampling may not be helpful in diagnostics of melanoma or parapsoriasis. Moreover, microbiome changes seem not to play a role in parapsoriasis. We could demonstrate that very low birth weight infants in intensive care have a high cutaneous microbial diversity during the first days of life regardless of the way of delivery, prematurity causes or perinatal infections. The diversity decreases during the first weeks of life possibly due to intensive care treatment and antibiotics. Neonatal sepsis is not linked to microbiome changes and it is likely that other factors play a role, e.g. skin injury by medical devices and concomitant infections.

AB - The skin microbiome – Investigations on skin malignancies and preterm newborn skin Skin disorders have been associated with specific microbiome changes and raised an interest in developing new diagnostic methods and treatments. Our object was to investigate the microbiome in skin cancer (melanoma) and inflammatory skin disorders (parapsoriasis) and to explore the skin microbiome in very low birth weight infants in intensive care and possible association to neonatal sepsis. Microbiome samples were taken of 15 cutaneous melanomas and 17 benign melanocytic nevi, of 13 patients with parapsoriasis and 12 very low birth weight infants during treatment in intensive care. Sequencing was carried out on 454 GS-FLX Titanium and Illumina MiSeq platforms and the data was analyzed by bioinformatics. There were no significant differences in the microbiome of melanomas, melanocytic nevi and controls. Additionally, the microbiome showed no significant differences between parapsoriasis and the same patient’s healthy skin. We observed a high cutaneous microbial diversity in most of the infants at birth and there was a decrease in diversity during the first three weeks of life. There was no association between microbiome changes and neonatal sepsis. The results suggest that microbiome swab sampling may not be helpful in diagnostics of melanoma or parapsoriasis. Moreover, microbiome changes seem not to play a role in parapsoriasis. We could demonstrate that very low birth weight infants in intensive care have a high cutaneous microbial diversity during the first days of life regardless of the way of delivery, prematurity causes or perinatal infections. The diversity decreases during the first weeks of life possibly due to intensive care treatment and antibiotics. Neonatal sepsis is not linked to microbiome changes and it is likely that other factors play a role, e.g. skin injury by medical devices and concomitant infections.

KW - Infant, Premature

KW - Skin

KW - +microbiology

KW - Microbiota

KW - Melanoma

KW - Parapsoriasis

KW - Nevus, Pigmented

KW - Skin Neoplasms

KW - Infant, Very Low Birth Weight

KW - Infant, Low Birth Weight

KW - Neonatal Sepsis

KW - Enterotoxins

KW - Lymphoma, T-Cell

KW - Mycosis Fungoides

KW - Staphylococcal Infections

KW - Staphylococcus aureus

KW - Intensive Care, Neonatal

KW - 3121 Internal medicine

M3 - Doctoral Thesis

SN - 978-951-51-3156-0

PB - [A. Salava]

CY - Helsinki

ER -