Three dimensional electron microscopy of cellular organelles by serial block face SEM and ET

Research output: Contribution to journalArticleScientific

Abstract

Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is needed. In this article we present examples of utilizing these two 3D-EM techniques to study cellular organelles.
Original languageEnglish
JournalMicroscopy and Analysis
Volume27
Issue number2
Pages (from-to)7-10
Number of pages4
ISSN2049-4424
Publication statusPublished - 2013
MoE publication typeB1 Journal article

Fields of Science

  • 1182 Biochemistry, cell and molecular biology

Cite this

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title = "Three dimensional electron microscopy of cellular organelles by serial block face SEM and ET",
abstract = "Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is needed. In this article we present examples of utilizing these two 3D-EM techniques to study cellular organelles.",
keywords = "1182 Biochemistry, cell and molecular biology",
author = "Helena Vihinen and Ilya Belevich and Eija Jokitalo",
year = "2013",
language = "English",
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pages = "7--10",
journal = "Microscopy and Analysis",
issn = "2049-4424",
publisher = "John Wiley & Sons Ltd.",
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TY - JOUR

T1 - Three dimensional electron microscopy of cellular organelles by serial block face SEM and ET

AU - Vihinen, Helena

AU - Belevich, Ilya

AU - Jokitalo, Eija

PY - 2013

Y1 - 2013

N2 - Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is needed. In this article we present examples of utilizing these two 3D-EM techniques to study cellular organelles.

AB - Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is needed. In this article we present examples of utilizing these two 3D-EM techniques to study cellular organelles.

KW - 1182 Biochemistry, cell and molecular biology

M3 - Article

VL - 27

SP - 7

EP - 10

JO - Microscopy and Analysis

JF - Microscopy and Analysis

SN - 2049-4424

IS - 2

ER -