Transcript analysis of commercial prostate cancer risk stratification panels in hard‐to‐predict grade group 2–4 prostate cancers

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Background Improved prognostication is needed to minimize overtreatment in grade group (GG) 2–4 prostate cancer. Our aim was to determine, at messenger RNA (mRNA) level, the performance of the genes in the commercial panels Decipher, Oncotype DX, Prolaris, and mutational panel MSK‐IMPACT to predict metastasis‐free and prostate cancer‐specific death (PCSD) in patients with GG 2–4 prostate cancer at radical prostatectomy. Methods The retrospective cohort consisted of GG 2–4 patients treated with radical prostatectomy (median follow‐up 10.4 years). Seventy‐six cases with postoperative metastasis or PCSD and 84 controls with similar clinical baseline risk, but without progression, were analyzed. Index lesion mRNA transcripts were analyzed using NanoString technology. Random forest models were trained using panel gene sets to predict clinical endpoints and area under the curve (AUC), sensitivity, specificity, Youden index, and number needed to diagnose (NND) was measured. Survival probability was assessed with Kaplan–Meier estimator. Results All gene sets outperformed clinical parameters and predicted metastasis‐free and prostate cancer‐specific survival. However, there were significant differences between the panels. In metastasis prediction, the genes in Oncotype DX had inferior performance (area under the curve [AUC] = 0.65) compared to other panels (AUC = 0.73–0.74). Decipher, MSK‐IMPACT and Prolaris showed similar NND (2.83–3.12) with Oncotype DX having highest NND (4.79). In PCSD prediction, the Prolaris gene set performed worse (AUC = 0.66) than MSK‐IMPACT or Decipher (AUC = 0.72). Oncotype DX performed similarly to other panels (AUC = 0.69, p > .05). Oncotype DX demonstrated lowest NND (2.79) compared to other panels (4.22–5.66). Conclusion Transcript analysis of genes included in commercial panels is feasible in survival prediction of GG 2‐4 patients after radical prostatectomy and may aid in clinical decision making. There were significant differences between the panels, and overall stronger predictive gene sets are needed. Prospective investigation is warranted in biopsy materials.
Original languageEnglish
JournalProstate
Volume81
Issue number7
Pages (from-to)368-376
Number of pages9
ISSN0270-4137
DOIs
Publication statusPublished - 15 May 2021
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 3122 Cancers
  • biomarker
  • decipher
  • gene panel
  • oncotype DX
  • prolaris

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