Uncoupling high light responses from singlet oxygen retrograde signaling and spatial-temporal systemic acquired acclimation

Melanie Carmody, Peter Crisp, Stefano d'Alessandro, Diep Ganguly, Matthew Gordon, Michel Havaux, Veronica Albrecht-Borth, Barry Pogson

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Distinct ROS signaling pathways initiated by singlet oxygen or superoxide and hydrogen peroxide (H2O2) have been attributed to either cell death or acclimation, respectively. Recent studies have revealed that more complex antagonistic and synergistic relationships exist within and between these pathways. As specific chloroplastic ROS signals are difficult to study, rapid systemic signaling experiments using localized high light (HL) stress or ROS treatments were used in this study to uncouple signals required for direct HL and ROS perception and distal systemic acquired acclimation (SAA). A qPCR approach was chosen to determine local perception and distal signal reception. Analysis of a thylakoidal ascorbate peroxidase mutant (tapx), the 1O2-retrograde signaling double mutant (ex1/ex2), and an apoplastic signaling double mutant (rbohD/F) revealed that tAPX and EXECUTER 1 are required for both HL and systemic acclimation stress perception. Apoplastic membrane-localized RBOHs were required for systemic spread of the signal but not for local signal induction in directly stressed tissues. Endogenous ROS treatments revealed a very strong systemic response induced by a localized 1 hour induction of 1O2 using the conditional flu mutant. A qPCR time course of 1O2 induced systemic marker genes in directly and indirectly connected leaves revealed a direct vascular connection component of both immediate and longer term SAA signaling responses. These results reveal the importance of an EXECUTER-dependent 1O2 retrograde signal for both local and long distance RBOH-dependent acclimation signaling that is distinct from other HL signaling pathways, and that direct vascular connections have a role in spatial-temporal SAA induction.
Original languageEnglish
JournalPlant Physiology
Volume171
Issue number3
Pages (from-to)1734-1749
Number of pages16
ISSN0032-0889
DOIs
Publication statusPublished - 10 Jun 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • ROS signaling
  • ROS
  • systemic signaling
  • acclimation
  • SINGLET OXYGEN
  • Hydrogen peroxide
  • Rboh (respiratory burst oxidase homologue)

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