Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine

Oula Penate-Medina; Robert J. Tower; Tuula Penate-Medina; Olga Will; Per E. J. Saris; Juho Suojanen; Timo Sorsa; Laura Huuskonen; Kaisa Hiippala; Reetta Satokari; Claus C. Gluer; Willem M. de Vos; Justus Reunanen

doi:10.1186/s12866-019-1538-z

Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine

Oula Penate-Medina, Robert J. Tower, Tuula Penate-Medina, Olga Will, Per E. J. Saris, Juho Suojanen, Timo Sorsa, Laura Huuskonen, Kaisa Hiippala, Reetta Satokari, Claus C. Gluer, Willem M. de Vos, Justus Reunanen

Research output: Contribution to journal › Article › Scientific › peer-review

Original language	English
Article number	167
Journal	BMC Microbiology
Volume	19
Number of pages	9
ISSN	1471-2180
DOIs	https://doi.org/10.1186/s12866-019-1538-z
Publication status	Published - 18 Jul 2019
MoE publication type	A1 Journal article-refereed

Fields of Science

Bacterial colonization
Fluorescence imaging
Katushka
Dual-color imaging
Three-dimensional
Fluorescence molecular tomography
Murine model
Far-red fluorescent protein
In vivo
Intestine
ESCHERICHIA-COLI
GUT MICROBIOTA
LACTOBACILLUS-PLANTARUM
LACTOCOCCUS-LACTIS
IN-VITRO
PERSISTENCE
DISEASE
DIET
1183 Plant biology, microbiology, virology

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Cite this

Penate-Medina, O., Tower, R. J., Penate-Medina, T., Will, O., Saris, P. E. J., Suojanen, J., Sorsa, T., Huuskonen, L., Hiippala, K., Satokari, R., Gluer, C. C., de Vos, W. M., & Reunanen, J. (2019). Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine. BMC Microbiology, 19, Article 167. https://doi.org/10.1186/s12866-019-1538-z

@article{d27c1470b2204d49ba17097c32c81139,

title = "Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine",

keywords = "Bacterial colonization, Fluorescence imaging, Katushka, Dual-color imaging, Three-dimensional, Fluorescence molecular tomography, Murine model, Far-red fluorescent protein, In vivo, Intestine, ESCHERICHIA-COLI, GUT MICROBIOTA, LACTOBACILLUS-PLANTARUM, LACTOCOCCUS-LACTIS, IN-VITRO, PERSISTENCE, DISEASE, DIET, 1183 Plant biology, microbiology, virology",

author = "Oula Penate-Medina and Tower, {Robert J.} and Tuula Penate-Medina and Olga Will and Saris, {Per E. J.} and Juho Suojanen and Timo Sorsa and Laura Huuskonen and Kaisa Hiippala and Reetta Satokari and Gluer, {Claus C.} and {de Vos}, {Willem M.} and Justus Reunanen",

year = "2019",

month = jul,

day = "18",

doi = "10.1186/s12866-019-1538-z",

language = "English",

volume = "19",

journal = "BMC Microbiology",

issn = "1471-2180",

publisher = "BMC",

}

Penate-Medina, O, Tower, RJ, Penate-Medina, T, Will, O, Saris, PEJ , Suojanen, J , Sorsa, T, Huuskonen, L, Hiippala, K , Satokari, R, Gluer, CC, de Vos, WM & Reunanen, J 2019, 'Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine', BMC Microbiology, vol. 19, 167. https://doi.org/10.1186/s12866-019-1538-z

Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine. / Penate-Medina, Oula; Tower, Robert J.; Penate-Medina, Tuula et al.
In: BMC Microbiology, Vol. 19, 167, 18.07.2019.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine

AU - Penate-Medina, Oula

AU - Tower, Robert J.

AU - Penate-Medina, Tuula

AU - Will, Olga

AU - Saris, Per E. J.

AU - Suojanen, Juho

AU - Sorsa, Timo

AU - Huuskonen, Laura

AU - Hiippala, Kaisa

AU - Satokari, Reetta

AU - Gluer, Claus C.

AU - de Vos, Willem M.

AU - Reunanen, Justus

PY - 2019/7/18

Y1 - 2019/7/18

KW - Bacterial colonization

KW - Fluorescence imaging

KW - Katushka

KW - Dual-color imaging

KW - Three-dimensional

KW - Fluorescence molecular tomography

KW - Murine model

KW - Far-red fluorescent protein

KW - In vivo

KW - Intestine

KW - ESCHERICHIA-COLI

KW - GUT MICROBIOTA

KW - LACTOBACILLUS-PLANTARUM

KW - LACTOCOCCUS-LACTIS

KW - IN-VITRO

KW - PERSISTENCE

KW - DISEASE

KW - DIET

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1186/s12866-019-1538-z

DO - 10.1186/s12866-019-1538-z

M3 - Article

SN - 1471-2180

VL - 19

JO - BMC Microbiology

JF - BMC Microbiology

M1 - 167

ER -

Penate-Medina O, Tower RJ, Penate-Medina T, Will O, Saris PEJ , Suojanen J et al. Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine. BMC Microbiology. 2019 Jul 18;19:167. doi: 10.1186/s12866-019-1538-z