Variation in leaf morpho-physiological traits in a world collection of faba bean germplasm

Hamid Khazaei, Arja Santanen, Fred Stoddard, Ken Street

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review


Water deficit is the major environmental constraint to the production and yield stability of faba bean (Vicia faba L.). Leaf traits and related physiological traits could be used for screening faba beans to achieve drought tolerance. A set of 400 faba bean accessions was chosen according to the principles of "Focused Identification of Germplasm Strategy (FIGS)" with the aim of identifying different kinds of drought-adaptation traits from 200 dry-adapted and 200 wet-adapted accessions. The experiment is conducted in well watered greenhouse conditions in a randomized complete block design with 4 replicates of single plants. Morphological and physiological traits including stomatal frequency and size, leaf gas exchange, leaf area and leaf temperature have been examined on leaves of consistent age in the wet-adapted accessions and all showed significant variation among accessions (P<0.001). Stomatal frequency varied from 29.4 to 91.4 per mm2, stomatal length from 42.0 to 62.8 µm and stomatal width from 25.8 to 47.2 μm. Stomatal frequency was strongly and negatively correlated to stomatal size (P<0.001) and to leaf area (P<0.001). Stomatal conductance varied between 0.043 and 0.618 mol m-2 s-1 and showed a strong and positive correlation with stomatal length (r = 0.68, P<0.001). The dry-adapted germplasm is currently under evaluation. Biochemical traits, including leaf sugar content and wax content, are being determined on samples taken from both sets. Broader ranges of morpho-physiological traits have been measured in this germplasm set than have been reported from smaller surveys in the literature. Stomatal conductance is the most informative of the stomatal traits and it remains to be seen which of the biochemical traits is most informative.
Original languageEnglish
Title of host publication24th NJF Congress
EditorsJan Hultgren, Paula Persson, Elisabet Nadean, Fredrik Fogelberg
Number of pages1
Volume7, No 3
Place of PublicationUppsala
Publication date2011
ISBN (Print)1653-2015
Publication statusPublished - 2011
MoE publication typeA4 Article in conference proceedings
Event24th NJF Congress and 2nd Nordic Feed Science Confereance - Uppsala, Sweden
Duration: 14 Jun 201116 Jun 2011

Fields of Science

  • 4111 Agronomy
  • 414 Agricultural biotechnology

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