Vesicle-like virion of Haloarcula hispanica pleomorphic virus 3 preserves high infectivity in saturated salt

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Hypersaline environments that are subject to salinity changes are particularly rich in viruses. Here we report a newly isolated archaeal halovirus, Haloarcula hispanica pleomorphic virus 3 (HHPV3). Its reproduction significantly retards host growth and decreases cell viability without causing lysis. HHPV3 particles require a minimum of 3 M NaCI for stability and maintain high infectivity even in saturated salt. Notably, virions are irreversibly inactivated at similar to 1.5 M NaCl in neutral pH, but tolerate this salinity at alkaline pH. The HHPV3 virion is a pleomorphic membrane vesicle containing two major protein species and lipids acquired nonselectively from the host membrane. The circular double-stranded DNA genome contains a conserved gene block characteristic of pleolipoviruses. We propose that HHPV3 is a member of the Betapleolipovirus genus (family Pleolipoviridae). Our findings add insights into the diversity observed among the pleolipoviruses found in hypersaline environments
Original languageEnglish
JournalVirology
Volume499
Pages (from-to)40-51
Number of pages12
ISSN0042-6822
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • Halovirus
  • Pleomorphic virus
  • Virus life cycle
  • Genome sequence
  • Pleolipoviridae
  • Archaeal virus
  • Hypersalinity
  • MEMBRANE-VESICLES
  • HOST INTERACTIONS
  • VIRAL UNIVERSE
  • ARCHAEA
  • GENOME
  • IDENTIFICATION
  • ENVIRONMENTS
  • SH1
  • HALOVIRUSES
  • EUKARYOTES

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