Whole-Cell Bioreporters for the Detection of Bioavailable Metals

Research output: Contribution to journalReview ArticleScientificpeer-review

Abstract

Whole-cell bioreporters are living microorganisms that produce a specific, quantifiable output in response to target chemicals. Typically, whole-cell bioreporters combine a sensor element for the substance of interest and a reporter element coding for an easily detectable protein. The sensor element is responsible for recognizing the presence of an analyte. In the case of metal bioreporters, the sensor element consists of a DNA promoter region for a metal-binding transcription factor fused to a promoterless reporter gene that encodes a signal-producing protein. In this review, we provide an overview of specific whole-cell bioreporters for heavy metals. Because the sensing of metals by bioreporter microorganisms is usually based on heavy metal resistance/homeostasis mechanisms, the basis of these mechanisms will also be discussed. The goal here is not to present a comprehensive summary of individual metal-specific bioreporters that have been constructed, but rather to express views on the theory and applications of metal-specific bioreporters and identify some directions for future research and development.
Original languageEnglish
JournalAdvances in Biochemical Engineering, Biotechnology
Volume118
Pages (from-to)31-63
Number of pages33
ISSN0724-6145
DOIs
Publication statusPublished - 2010
MoE publication typeA2 Review article in a scientific journal

Fields of Science

  • 1182 Biochemistry, cell and molecular biology

Cite this

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title = "Whole-Cell Bioreporters for the Detection of Bioavailable Metals",
abstract = "Whole-cell bioreporters are living microorganisms that produce a specific, quantifiable output in response to target chemicals. Typically, whole-cell bioreporters combine a sensor element for the substance of interest and a reporter element coding for an easily detectable protein. The sensor element is responsible for recognizing the presence of an analyte. In the case of metal bioreporters, the sensor element consists of a DNA promoter region for a metal-binding transcription factor fused to a promoterless reporter gene that encodes a signal-producing protein. In this review, we provide an overview of specific whole-cell bioreporters for heavy metals. Because the sensing of metals by bioreporter microorganisms is usually based on heavy metal resistance/homeostasis mechanisms, the basis of these mechanisms will also be discussed. The goal here is not to present a comprehensive summary of individual metal-specific bioreporters that have been constructed, but rather to express views on the theory and applications of metal-specific bioreporters and identify some directions for future research and development.",
keywords = "1182 Biochemistry, cell and molecular biology",
author = "Anu Hynninen and Marko Virta",
year = "2010",
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volume = "118",
pages = "31--63",
journal = "Advances in Biochemical Engineering, Biotechnology",
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publisher = "Springer-Verlag",

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Whole-Cell Bioreporters for the Detection of Bioavailable Metals. / Hynninen, Anu; Virta, Marko.

In: Advances in Biochemical Engineering, Biotechnology, Vol. 118, 2010, p. 31-63.

Research output: Contribution to journalReview ArticleScientificpeer-review

TY - JOUR

T1 - Whole-Cell Bioreporters for the Detection of Bioavailable Metals

AU - Hynninen, Anu

AU - Virta, Marko

PY - 2010

Y1 - 2010

N2 - Whole-cell bioreporters are living microorganisms that produce a specific, quantifiable output in response to target chemicals. Typically, whole-cell bioreporters combine a sensor element for the substance of interest and a reporter element coding for an easily detectable protein. The sensor element is responsible for recognizing the presence of an analyte. In the case of metal bioreporters, the sensor element consists of a DNA promoter region for a metal-binding transcription factor fused to a promoterless reporter gene that encodes a signal-producing protein. In this review, we provide an overview of specific whole-cell bioreporters for heavy metals. Because the sensing of metals by bioreporter microorganisms is usually based on heavy metal resistance/homeostasis mechanisms, the basis of these mechanisms will also be discussed. The goal here is not to present a comprehensive summary of individual metal-specific bioreporters that have been constructed, but rather to express views on the theory and applications of metal-specific bioreporters and identify some directions for future research and development.

AB - Whole-cell bioreporters are living microorganisms that produce a specific, quantifiable output in response to target chemicals. Typically, whole-cell bioreporters combine a sensor element for the substance of interest and a reporter element coding for an easily detectable protein. The sensor element is responsible for recognizing the presence of an analyte. In the case of metal bioreporters, the sensor element consists of a DNA promoter region for a metal-binding transcription factor fused to a promoterless reporter gene that encodes a signal-producing protein. In this review, we provide an overview of specific whole-cell bioreporters for heavy metals. Because the sensing of metals by bioreporter microorganisms is usually based on heavy metal resistance/homeostasis mechanisms, the basis of these mechanisms will also be discussed. The goal here is not to present a comprehensive summary of individual metal-specific bioreporters that have been constructed, but rather to express views on the theory and applications of metal-specific bioreporters and identify some directions for future research and development.

KW - 1182 Biochemistry, cell and molecular biology

U2 - 10.1007/10_2009_9

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M3 - Review Article

VL - 118

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JO - Advances in Biochemical Engineering, Biotechnology

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