Immunochemical analysis of oat prolamins in European cultivars

Ahola, H. (Puhuja)

Aktiviteetti: Puhe- tai esitystyypitSuullinen esitys

Kuvaus

Celiac disease (CD) can be defined as a multisystem immune based disorder mainly triggered by ingested gluten. The development of the disease can lead to severe gastrointestinal symptoms, metabolic bone disease and infertility. At the moment, the only treatment for CD is a strict gluten free diet, which may feel challenging in various ways. Many clinical trials in Finland and Northern Europe have shown that pure oats are a suitable and palatable addition to long-term gluten free diets for both children and adult patients. Additionally, as oats are high in dietary fibre and hold several nutritional claims, adding oats to the CD patients’ gluten free diets is a feasible way of adding much needed dietary fibre. Nonetheless, there is still debate whether all oat cultivars are safe for CD patients. To ensure using oats is safe for CD patients, variations in composition and immunogenicity of oat prolamins (avenins) need to be investigated.

In this study, 26 European oat cultivars were characterised and their immunological reactivity studied. Some of the cultivars have been recorded to have a higher affinity to immunological assays and to possibly contain T-cell activating epitopes. Avenins have been characterised and grouped first qualitatively with SDS-PAGE and then quantitatively with ELISA (enzyme-linked immunosorbent assay).

Future research will include further immunological tests (e.g. immunoblotting) as well as separating and further characterising oat cultivar avenins by HPLC and electrophoresis.
Aikajakso16 huhtikuuta 2019
Tapahtuman otsikko18th European Young Cereal Scientist and Technologist Workshop
Tapahtuman tyyppiTyöpaja
SijaintiSan Benedetto del Tronto, Italia
Tunnustuksen arvoKansainvälinen