A feed-forward loop coupling extracellular BMP transport and morphogenesis in Drosophila wing

Shinya Matsuda, Jorge Blanco, Osamu Shimmi

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Abstrakti

A variety of extracellular factors regulate morphogenesis during development.
However, coordination between extracellular signaling and dynamic morphogenesis
is largely unexplored. We address the fundamental question by studying posterior
crossvein (PCV) development in Drosophila as a model, in which long-range BMP
transport from the longitudinal veins plays a critical role during the pupal
stages. Here, we show that RhoGAP Crossveinless-C (Cv-C) is induced at the PCV
primordial cells by BMP signaling and mediates PCV morphogenesis
cell-autonomously by inactivating members of the Rho-type small GTPases.
Intriguingly, we find that Cv-C is also required non-cell-autonomously for BMP
transport into the PCV region, while a long-range BMP transport is guided toward
ectopic wing vein regions by loss of the Rho-type small GTPases. We present
evidence that low level of ß-integrin accumulation at the basal side of PCV
epithelial cells regulated by Cv-C provides an optimal extracellular environment
for guiding BMP transport. These data suggest that BMP transport and PCV
morphogenesis are tightly coupled. Our study reveals a feed-forward mechanism
that coordinates the spatial distribution of extracellular instructive cues and
morphogenesis. The coupling mechanism may be widely utilized to achieve precise
morphogenesis during development and homeostasis.
Alkuperäiskielienglanti
Artikkelie1003403
LehtiPLoS Genetics
Vuosikerta9
Numero3
Sivumäärä11
ISSN1553-7390
DOI - pysyväislinkit
TilaJulkaistu - maaliskuuta 2013
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1184 Genetiikka, kehitysbiologia, fysiologia

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