Ability of minus strands and modified plus strands to act as templates in Semliki Forest virus RNA replication.

Kirsi Johanna Hellström, Katri Anneli Kallio, Hanna-Mari Meriläinen, Eija Sofia Jokitalo, Tero Tapani Ahola

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Abstrakti

During virus multiplication, the viral genome is recognized and recruited for replication based on specific cis-acting elements. Here we have dissected the important cis-acting sequence elements in Semliki Forest virus RNA, by using a trans-replication system. Since the viral replicase is expressed from a separate plasmid, the template RNA can be freely modified in this system. We show that the cis-acting element at the beginning of the non-structural protein 1 (nsP1) coding region together with the end of the 3′ untranslated region are the minimal requirements for minus strand synthesis. To achieve a high level of replication, the native 5′ untranslated region was also needed. The virus-induced membranous replication compartments or spherules were only detected when a replication-competent template was present with an active replicase and minus strands were produced. No translation could be detected from the minus strands, suggesting that they are segregated from the cytoplasm. Minus strands could not directly be recruited to initiate the replication process. Thus, there is only one defined pathway for replication, starting with plus strand recognition followed by concomitant spherule formation and minus strand synthesis.
Alkuperäiskielienglanti
LehtiJournal of General Virology
Vuosikerta87
Numero6
Sivut1395-1407
Sivumäärä13
ISSN0022-1317
DOI - pysyväislinkit
TilaJulkaistu - 2016
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1183 Kasvibiologia, mikrobiologia, virologia

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