Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue

Pinky Dutta; Kristiina Mäkinen

doi:10.1007/978-1-0716-3485-1_7

Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue

Tutkimustuotos: Artikkeli kirjassa/raportissa/konferenssijulkaisussa › Kirjan luku tai artikkeli › Tieteellinen › vertaisarvioitu

Abstrakti

Reverse transcription quantitative PCR (RT-qPCR) allows sensitive and specific measurement of mRNA transcripts from a given sample in a short period of time. Relative and absolute RT-qPCR are two strategies that could be used to quantify mRNA transcripts, based on the goal of the experiment. Here, we describe the protocol for the quantification of plant viral RNA transcripts from an infected sample using both strategies.

Alkuperäiskieli	englanti
Otsikko	Plant-Virus Interactions
Toimittajat	Elizabeth P.B. Fontes, Kristiina Mäkinen
Sivumäärä	11
Julkaisupaikka	New York
Kustantaja	Humana press
Julkaisupäivä	2024
Sivut	81-91
ISBN (painettu)	978-1-0716-3484-4, 978-1-0716-3487-5
ISBN (elektroninen)	978-1-0716-3485-1
DOI - pysyväislinkit	https://doi.org/10.1007/978-1-0716-3485-1_7
Tila	Julkaistu - 2024
OKM-julkaisutyyppi	A3 Kirjan tai muun kokoomateoksen osa

Julkaisusarja

Nimi	Methods in Molecular Biology
Vuosikerta	2724
ISSN (painettu)	1064-3745
ISSN (elektroninen)	1940-6029

Lisätietoja

Funding Information:
We thank the Academy of Finland (Grant 1332950) for KM and Doctoral Programme in Plant Sciences, University of Helsinki, for PD for financial support.

Tieteenalat

11831 Kasvibiologia
4111 Maataloustiede

Pääsy asiakirjaan

10.1007/978-1-0716-3485-1_7Lisenssi: Määrittelemätön

Final_draft_Absolute and relative quantification of ssRNA virus-1Hyväksytty kirjoittajan käsikirjoitus, 165 KBLisenssi: Määrittelemätön

Siteeraa tätä

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Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue. / Dutta, Pinky ; Mäkinen, Kristiina.
Plant-Virus Interactions. toim. / Elizabeth P.B. Fontes; Kristiina Mäkinen. New York: Humana press, 2024. s. 81-91 (Methods in Molecular Biology; Vuosikerta 2724).

Tutkimustuotos: Artikkeli kirjassa/raportissa/konferenssijulkaisussa › Kirjan luku tai artikkeli › Tieteellinen › vertaisarvioitu

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