Accurate Calibration in Multi-Material 3D Bioprinting for Tissue Engineering

Enrique Sodupe-Ortega, Andres Sanz-Garcia, Alpha Pernia-Espinoza, Carmen Escobedo-Lucea

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Most of the studies in three-dimensional (3D) bioprinting have been traditionally based on printing a single bioink. Addressing the complexity of organ and tissue engineering, however, will require combining multiple building and sacrificial biomaterials and several cells types in a single biofabrication session. This is a significant challenge, and, to tackle that, we must focus on the complex relationships between the printing parameters and the print resolution. In this paper, we study the influence of the main parameters driven multi-material 3D bioprinting and we present a method to calibrate these systems and control the print resolution accurately. Firstly, poloxamer hydrogels were extruded using a desktop 3D printer modified to incorporate four microextrusion-based bioprinting (MEBB) printheads. The printed hydrogels provided us the particular range of printing parameters (mainly printing pressure, deposition speed, and nozzle z-offset) to assure the correct calibration of the multi-material 3D bioprinter. Using the printheads, we demonstrated the excellent performance of the calibrated system extruding different fluorescent bioinks. Representative multi-material structures were printed in both poloxamer and cell-laden gelatin-alginate bioinks in a single session corroborating the capabilities of our system and the calibration method. Cell viability was not significantly affected by any of the changes proposed. We conclude that our proposal has enormous potential to help with advancing in the creation of complex 3D constructs and vascular networks for tissue engineering.
Alkuperäiskielienglanti
Artikkeli1402
LehtiMaterials
Vuosikerta11
Numero8
Sivumäärä19
ISSN1996-1944
DOI - pysyväislinkit
TilaJulkaistu - 10 elokuuta 2018
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 220 Teollinen bioteknologia
  • 216 Materiaalitekniikka

Lainaa tätä

Sodupe-Ortega, Enrique ; Sanz-Garcia, Andres ; Pernia-Espinoza, Alpha ; Escobedo-Lucea, Carmen. / Accurate Calibration in Multi-Material 3D Bioprinting for Tissue Engineering. Julkaisussa: Materials. 2018 ; Vuosikerta 11, Nro 8.
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title = "Accurate Calibration in Multi-Material 3D Bioprinting for Tissue Engineering",
abstract = "Most of the studies in three-dimensional (3D) bioprinting have been traditionally based on printing a single bioink. Addressing the complexity of organ and tissue engineering, however, will require combining multiple building and sacrificial biomaterials and several cells types in a single biofabrication session. This is a significant challenge, and, to tackle that, we must focus on the complex relationships between the printing parameters and the print resolution. In this paper, we study the influence of the main parameters driven multi-material 3D bioprinting and we present a method to calibrate these systems and control the print resolution accurately. Firstly, poloxamer hydrogels were extruded using a desktop 3D printer modified to incorporate four microextrusion-based bioprinting (MEBB) printheads. The printed hydrogels provided us the particular range of printing parameters (mainly printing pressure, deposition speed, and nozzle z-offset) to assure the correct calibration of the multi-material 3D bioprinter. Using the printheads, we demonstrated the excellent performance of the calibrated system extruding different fluorescent bioinks. Representative multi-material structures were printed in both poloxamer and cell-laden gelatin-alginate bioinks in a single session corroborating the capabilities of our system and the calibration method. Cell viability was not significantly affected by any of the changes proposed. We conclude that our proposal has enormous potential to help with advancing in the creation of complex 3D constructs and vascular networks for tissue engineering.",
keywords = "220 Industrial biotechnology, 216 Materials engineering, additive manufacturing, synthetic polymer, bioprinting, multi-material microextrusion, bioink, FABRICATION, HYDROGELS, SYSTEM",
author = "Enrique Sodupe-Ortega and Andres Sanz-Garcia and Alpha Pernia-Espinoza and Carmen Escobedo-Lucea",
year = "2018",
month = "8",
day = "10",
doi = "10.3390/ma11081402",
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journal = "Materials",
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Accurate Calibration in Multi-Material 3D Bioprinting for Tissue Engineering. / Sodupe-Ortega, Enrique; Sanz-Garcia, Andres; Pernia-Espinoza, Alpha; Escobedo-Lucea, Carmen.

julkaisussa: Materials, Vuosikerta 11, Nro 8, 1402, 10.08.2018.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Accurate Calibration in Multi-Material 3D Bioprinting for Tissue Engineering

AU - Sodupe-Ortega, Enrique

AU - Sanz-Garcia, Andres

AU - Pernia-Espinoza, Alpha

AU - Escobedo-Lucea, Carmen

PY - 2018/8/10

Y1 - 2018/8/10

N2 - Most of the studies in three-dimensional (3D) bioprinting have been traditionally based on printing a single bioink. Addressing the complexity of organ and tissue engineering, however, will require combining multiple building and sacrificial biomaterials and several cells types in a single biofabrication session. This is a significant challenge, and, to tackle that, we must focus on the complex relationships between the printing parameters and the print resolution. In this paper, we study the influence of the main parameters driven multi-material 3D bioprinting and we present a method to calibrate these systems and control the print resolution accurately. Firstly, poloxamer hydrogels were extruded using a desktop 3D printer modified to incorporate four microextrusion-based bioprinting (MEBB) printheads. The printed hydrogels provided us the particular range of printing parameters (mainly printing pressure, deposition speed, and nozzle z-offset) to assure the correct calibration of the multi-material 3D bioprinter. Using the printheads, we demonstrated the excellent performance of the calibrated system extruding different fluorescent bioinks. Representative multi-material structures were printed in both poloxamer and cell-laden gelatin-alginate bioinks in a single session corroborating the capabilities of our system and the calibration method. Cell viability was not significantly affected by any of the changes proposed. We conclude that our proposal has enormous potential to help with advancing in the creation of complex 3D constructs and vascular networks for tissue engineering.

AB - Most of the studies in three-dimensional (3D) bioprinting have been traditionally based on printing a single bioink. Addressing the complexity of organ and tissue engineering, however, will require combining multiple building and sacrificial biomaterials and several cells types in a single biofabrication session. This is a significant challenge, and, to tackle that, we must focus on the complex relationships between the printing parameters and the print resolution. In this paper, we study the influence of the main parameters driven multi-material 3D bioprinting and we present a method to calibrate these systems and control the print resolution accurately. Firstly, poloxamer hydrogels were extruded using a desktop 3D printer modified to incorporate four microextrusion-based bioprinting (MEBB) printheads. The printed hydrogels provided us the particular range of printing parameters (mainly printing pressure, deposition speed, and nozzle z-offset) to assure the correct calibration of the multi-material 3D bioprinter. Using the printheads, we demonstrated the excellent performance of the calibrated system extruding different fluorescent bioinks. Representative multi-material structures were printed in both poloxamer and cell-laden gelatin-alginate bioinks in a single session corroborating the capabilities of our system and the calibration method. Cell viability was not significantly affected by any of the changes proposed. We conclude that our proposal has enormous potential to help with advancing in the creation of complex 3D constructs and vascular networks for tissue engineering.

KW - 220 Industrial biotechnology

KW - 216 Materials engineering

KW - additive manufacturing

KW - synthetic polymer

KW - bioprinting

KW - multi-material microextrusion

KW - bioink

KW - FABRICATION

KW - HYDROGELS

KW - SYSTEM

U2 - 10.3390/ma11081402

DO - 10.3390/ma11081402

M3 - Article

VL - 11

JO - Materials

JF - Materials

SN - 1996-1944

IS - 8

M1 - 1402

ER -