Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds

Rubina Ajdary, Siqi Huan, Nazanin Zanjanizadeh Ezazi, Wenchao Xiang, Rafael Grande, Hélder A. Santos, Orlando J. Rojas

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity with extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits 3D printing into scaffolds that support cell attachment and proliferation. Here we propose aqueous suspensions of acetylated nanocellulose of low degree of substitution for direct ink writing (DIW). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D-printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally-stable, monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scale up fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering.
Alkuperäiskielienglanti
LehtiBiomacromolecules
Vuosikerta20
Numero7
Sivut2770-2778
Sivumäärä9
ISSN1525-7797
DOI - pysyväislinkit
TilaJulkaistu - 22 toukokuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Lainaa tätä

Ajdary, Rubina ; Huan, Siqi ; Zanjanizadeh Ezazi, Nazanin ; Xiang, Wenchao ; Grande, Rafael ; Santos, Hélder A. ; Rojas, Orlando J. / Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds. Julkaisussa: Biomacromolecules. 2019 ; Vuosikerta 20, Nro 7. Sivut 2770-2778 .
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title = "Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds",
abstract = "Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity with extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits 3D printing into scaffolds that support cell attachment and proliferation. Here we propose aqueous suspensions of acetylated nanocellulose of low degree of substitution for direct ink writing (DIW). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D-printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally-stable, monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scale up fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering.",
author = "Rubina Ajdary and Siqi Huan and {Zanjanizadeh Ezazi}, Nazanin and Wenchao Xiang and Rafael Grande and Santos, {H{\'e}lder A.} and Rojas, {Orlando J.}",
year = "2019",
month = "5",
day = "22",
doi = "10.1021/acs.biomac.9b00527",
language = "English",
volume = "20",
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journal = "Biomacromolecules",
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publisher = "American Chemical Society",
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Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds. / Ajdary, Rubina; Huan, Siqi; Zanjanizadeh Ezazi, Nazanin; Xiang, Wenchao; Grande, Rafael ; Santos, Hélder A.; Rojas, Orlando J.

julkaisussa: Biomacromolecules, Vuosikerta 20, Nro 7, 22.05.2019, s. 2770-2778 .

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds

AU - Ajdary, Rubina

AU - Huan, Siqi

AU - Zanjanizadeh Ezazi, Nazanin

AU - Xiang, Wenchao

AU - Grande, Rafael

AU - Santos, Hélder A.

AU - Rojas, Orlando J.

PY - 2019/5/22

Y1 - 2019/5/22

N2 - Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity with extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits 3D printing into scaffolds that support cell attachment and proliferation. Here we propose aqueous suspensions of acetylated nanocellulose of low degree of substitution for direct ink writing (DIW). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D-printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally-stable, monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scale up fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering.

AB - Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity with extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits 3D printing into scaffolds that support cell attachment and proliferation. Here we propose aqueous suspensions of acetylated nanocellulose of low degree of substitution for direct ink writing (DIW). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D-printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally-stable, monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scale up fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering.

U2 - 10.1021/acs.biomac.9b00527

DO - 10.1021/acs.biomac.9b00527

M3 - Article

VL - 20

SP - 2770

EP - 2778

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 7

ER -