TY - JOUR
T1 - Acidipropionibacterium virtanenii sp. nov., isolated from malted barley
AU - Deptula, Paulina
AU - Smolander, Olli-Pekka Aukusti
AU - Laine, Pia Kati Sofia
AU - Roberts, Richard J.
AU - Edelmann, Johanna Minnamari
AU - Peltola, Petri
AU - Piironen, Vieno Irene
AU - Paulin, Lars Göran
AU - Storgårds, Erna
AU - Savijoki, Kirsi
AU - Laitila, Arja
AU - Auvinen, Petri Olli Viljami
AU - Varmanen, Pekka Kristian
PY - 2018/10
Y1 - 2018/10
N2 - A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278(T) to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278(T) is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37 degrees C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278(T) (=VTT E-113202(T)=DSM 106790(T)).
AB - A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278(T) to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278(T) is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37 degrees C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278(T) (=VTT E-113202(T)=DSM 106790(T)).
KW - A. acidipropionici
KW - A. jensenii
KW - Acidipropionibacterium virtanenii
KW - BACTERIOCIN
KW - CLASSIFICATION
KW - FREUDENREICHII
KW - GENES
KW - GENOMES
KW - JENSENII
KW - NEIGHBOR-JOINING METHOD
KW - PROPIONIBACTERIUM-THOENII
KW - RECLASSIFICATION
KW - TOOL
KW - defence mechanisms
KW - malted barley
KW - pseudovitamin B12
KW - 1183 Plant biology, microbiology, virology
U2 - 10.1099/ijsem.0.002965
DO - 10.1099/ijsem.0.002965
M3 - Article
SN - 1466-5026
VL - 68
SP - 3175
EP - 3183
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 10
ER -