Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.)

Eliška Nováková, Lenka Zablatzká , Jan Brus , Viktorie Nesrstová , Pavel Hanáček, Ruslan Kalendar, Fatima Cvrčková , Ľuboš Majeský , Petr Smýkal

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Reproductive isolation is an important component of species differentiation. The plastid accD gene coding for the acetyl-CoA carboxylase subunit and the nuclear bccp gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated (Pisum sativum, P. abyssinicum) and wild (P. fulvum and P. elatius) peas. Based on deduced protein sequences, we identified 34 accD and 31 bccp alleles that are partially geographically and genetically structured. The accD is highly variable due to insertions of tandem repeats. P. fulvum and P. abyssinicum have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between P. sativum and P. elatius. Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.
Alkuperäiskielienglanti
Artikkeli1773
LehtiInternational Journal of Molecular Sciences
Vuosikerta20
Numero7
Sivumäärä19
ISSN1422-0067
DOI - pysyväislinkit
TilaJulkaistu - 10 huhtikuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1184 Genetiikka, kehitysbiologia, fysiologia

Lainaa tätä

Nováková, Eliška ; Zablatzká , Lenka ; Brus , Jan ; Nesrstová , Viktorie ; Hanáček, Pavel ; Kalendar, Ruslan ; Cvrčková , Fatima ; Majeský , Ľuboš ; Smýkal, Petr. / Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.). Julkaisussa: International Journal of Molecular Sciences. 2019 ; Vuosikerta 20, Nro 7.
@article{4bb8e01710ee4e6580b2e590e671ea58,
title = "Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.)",
abstract = "Reproductive isolation is an important component of species differentiation. The plastid accD gene coding for the acetyl-CoA carboxylase subunit and the nuclear bccp gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated (Pisum sativum, P. abyssinicum) and wild (P. fulvum and P. elatius) peas. Based on deduced protein sequences, we identified 34 accD and 31 bccp alleles that are partially geographically and genetically structured. The accD is highly variable due to insertions of tandem repeats. P. fulvum and P. abyssinicum have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between P. sativum and P. elatius. Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.",
keywords = "1184 Genetics, developmental biology, physiology, EVOLUTIONARY GENETICS, FERTILITY, HYBRID INCOMPATIBILITY, INHERITANCE, MALE-STERILITY, MECHANISMS, PLANT, REPRODUCTIVE ISOLATION, SELECTION, SEQUENCES, acetyl-CoA carboxylase, hybrid incompatibility, hybrid necrosis, nuclear-cytoplasmic conflict, pea, reproductive isolation, speciation",
author = "Eliška Nov{\'a}kov{\'a} and Lenka Zablatzk{\'a} and Jan Brus and Viktorie Nesrstov{\'a} and Pavel Han{\'a}ček and Ruslan Kalendar and Fatima Cvrčkov{\'a} and Ľuboš Majesk{\'y} and Petr Sm{\'y}kal",
year = "2019",
month = "4",
day = "10",
doi = "10.3390/ijms20071773",
language = "English",
volume = "20",
journal = "International Journal of Molecular Sciences",
issn = "1422-0067",
publisher = "MDPI",
number = "7",

}

Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.). / Nováková, Eliška; Zablatzká , Lenka; Brus , Jan; Nesrstová , Viktorie; Hanáček, Pavel; Kalendar, Ruslan; Cvrčková , Fatima; Majeský , Ľuboš; Smýkal, Petr.

julkaisussa: International Journal of Molecular Sciences, Vuosikerta 20, Nro 7, 1773, 10.04.2019.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.)

AU - Nováková, Eliška

AU - Zablatzká , Lenka

AU - Brus , Jan

AU - Nesrstová , Viktorie

AU - Hanáček, Pavel

AU - Kalendar, Ruslan

AU - Cvrčková , Fatima

AU - Majeský , Ľuboš

AU - Smýkal, Petr

PY - 2019/4/10

Y1 - 2019/4/10

N2 - Reproductive isolation is an important component of species differentiation. The plastid accD gene coding for the acetyl-CoA carboxylase subunit and the nuclear bccp gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated (Pisum sativum, P. abyssinicum) and wild (P. fulvum and P. elatius) peas. Based on deduced protein sequences, we identified 34 accD and 31 bccp alleles that are partially geographically and genetically structured. The accD is highly variable due to insertions of tandem repeats. P. fulvum and P. abyssinicum have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between P. sativum and P. elatius. Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.

AB - Reproductive isolation is an important component of species differentiation. The plastid accD gene coding for the acetyl-CoA carboxylase subunit and the nuclear bccp gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated (Pisum sativum, P. abyssinicum) and wild (P. fulvum and P. elatius) peas. Based on deduced protein sequences, we identified 34 accD and 31 bccp alleles that are partially geographically and genetically structured. The accD is highly variable due to insertions of tandem repeats. P. fulvum and P. abyssinicum have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between P. sativum and P. elatius. Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.

KW - 1184 Genetics, developmental biology, physiology

KW - EVOLUTIONARY GENETICS

KW - FERTILITY

KW - HYBRID INCOMPATIBILITY

KW - INHERITANCE

KW - MALE-STERILITY

KW - MECHANISMS

KW - PLANT

KW - REPRODUCTIVE ISOLATION

KW - SELECTION

KW - SEQUENCES

KW - acetyl-CoA carboxylase

KW - hybrid incompatibility

KW - hybrid necrosis

KW - nuclear-cytoplasmic conflict

KW - pea

KW - reproductive isolation

KW - speciation

UR - https://www.mdpi.com/journal/ijms/special_issues/pea_legume

U2 - 10.3390/ijms20071773

DO - 10.3390/ijms20071773

M3 - Article

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 7

M1 - 1773

ER -