Alteration in Microbial Community in Creosote Contaminated Soils

Tutkimustuotos: KonferenssimateriaalitPosteriTutkimus

Kuvaus

Objective Long-term exposure to polyaromatic hydrocarbons (PAHs) in living environment has been connected to chronic health disorders in humans. Almost 90% of the PAHs emitted into the environment naturally or as a result of human activity end up in the top 20 cm of the soil. Microbial degradation and abiotic disappearance of PAHs favor certain microbial taxa, while others become less prevalent as a result of resource competition and direct PAH toxicity. Bacterial species that prosper under PAH exposure vary from soil to soil. Assuming that the goal is to understand microbial shifts in PAHs polluted soils, it is necessary to study microbial community changes in several soils concurrently. This study intended to study the bacterial communities that are altered as a result of creosote treatment and PAH degradation. The rationale behind our study is to facilitate reconsidering factors leading to bioremediation of creosote contaminated soils.
Methodology Large containers filled with either spruce forest soil, pine forest soil, peat or glacial sand were contaminated with creosote or left intact. The degradation of PAHs, primarily fluoranthene, phenanthrene and pyrene, was followed for 27 weeks. The degradation was monitored at 4, 13 and 27 weeks using gas chromatography-mass spectroscopy (GC-MS), and bacterial community composition was analysed using 454 pyrosequencing.
Results and Discussion Relative abundance and richness of Proteobacteria, particularly Betaproteobacteria and Deltaproteobacteria, was higher while Bacteroidetes and several genuses under the phylum Bacteroidetes including Sphingobacteriia was less abundant in creosote contaminated soils. This finding could be turn out to be important in bioremediation approach of creosote or PAH contaminated sites.
Alkuperäiskielienglanti
TilaJulkaistu - 2017
OKM-julkaisutyyppiEi sovellu
Tapahtuma6th Annual International Conference on Sustainable Energy and Environmental Science - Singapore, Singapore
Kesto: 6 maaliskuuta 20177 maaliskuuta 2017

Konferenssi

Konferenssi6th Annual International Conference on Sustainable Energy and Environmental Science
MaaSingapore
KaupunkiSingapore
Ajanjakso06/03/201707/03/2017

Tieteenalat

  • 1172 Ympäristötiede

Lainaa tätä

Parajuli, A., Sinkkonen, A. T., & Grönroos, K. M. M. (2017). Alteration in Microbial Community in Creosote Contaminated Soils. Posterin esittämispaikka: 6th Annual International Conference on Sustainable Energy and Environmental Science, Singapore, Singapore.
Parajuli, Anirudra ; Sinkkonen, Aki Tapio ; Grönroos, Kati Mira Marika. / Alteration in Microbial Community in Creosote Contaminated Soils. Posterin esittämispaikka: 6th Annual International Conference on Sustainable Energy and Environmental Science, Singapore, Singapore.
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title = "Alteration in Microbial Community in Creosote Contaminated Soils",
abstract = "Objective Long-term exposure to polyaromatic hydrocarbons (PAHs) in living environment has been connected to chronic health disorders in humans. Almost 90{\%} of the PAHs emitted into the environment naturally or as a result of human activity end up in the top 20 cm of the soil. Microbial degradation and abiotic disappearance of PAHs favor certain microbial taxa, while others become less prevalent as a result of resource competition and direct PAH toxicity. Bacterial species that prosper under PAH exposure vary from soil to soil. Assuming that the goal is to understand microbial shifts in PAHs polluted soils, it is necessary to study microbial community changes in several soils concurrently. This study intended to study the bacterial communities that are altered as a result of creosote treatment and PAH degradation. The rationale behind our study is to facilitate reconsidering factors leading to bioremediation of creosote contaminated soils. Methodology Large containers filled with either spruce forest soil, pine forest soil, peat or glacial sand were contaminated with creosote or left intact. The degradation of PAHs, primarily fluoranthene, phenanthrene and pyrene, was followed for 27 weeks. The degradation was monitored at 4, 13 and 27 weeks using gas chromatography-mass spectroscopy (GC-MS), and bacterial community composition was analysed using 454 pyrosequencing.Results and Discussion Relative abundance and richness of Proteobacteria, particularly Betaproteobacteria and Deltaproteobacteria, was higher while Bacteroidetes and several genuses under the phylum Bacteroidetes including Sphingobacteriia was less abundant in creosote contaminated soils. This finding could be turn out to be important in bioremediation approach of creosote or PAH contaminated sites.",
keywords = "1172 Environmental sciences, Creosote, BACTERIAL COMMUNITIES, Proteobacteria, Bacteroidetes, pristine soil",
author = "Anirudra Parajuli and Sinkkonen, {Aki Tapio} and Gr{\"o}nroos, {Kati Mira Marika}",
note = "Volume: Proceeding volume: ; 6th Annual International Conference on Sustainable Energy and Environmental Science ; Conference date: 06-03-2017 Through 07-03-2017",
year = "2017",
language = "English",

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Parajuli, A, Sinkkonen, AT & Grönroos, KMM 2017, 'Alteration in Microbial Community in Creosote Contaminated Soils' 6th Annual International Conference on Sustainable Energy and Environmental Science, Singapore, Singapore, 06/03/2017 - 07/03/2017, .

Alteration in Microbial Community in Creosote Contaminated Soils. / Parajuli, Anirudra; Sinkkonen, Aki Tapio; Grönroos, Kati Mira Marika.

2017. Posterin esittämispaikka: 6th Annual International Conference on Sustainable Energy and Environmental Science, Singapore, Singapore.

Tutkimustuotos: KonferenssimateriaalitPosteriTutkimus

TY - CONF

T1 - Alteration in Microbial Community in Creosote Contaminated Soils

AU - Parajuli, Anirudra

AU - Sinkkonen, Aki Tapio

AU - Grönroos, Kati Mira Marika

N1 - Volume: Proceeding volume:

PY - 2017

Y1 - 2017

N2 - Objective Long-term exposure to polyaromatic hydrocarbons (PAHs) in living environment has been connected to chronic health disorders in humans. Almost 90% of the PAHs emitted into the environment naturally or as a result of human activity end up in the top 20 cm of the soil. Microbial degradation and abiotic disappearance of PAHs favor certain microbial taxa, while others become less prevalent as a result of resource competition and direct PAH toxicity. Bacterial species that prosper under PAH exposure vary from soil to soil. Assuming that the goal is to understand microbial shifts in PAHs polluted soils, it is necessary to study microbial community changes in several soils concurrently. This study intended to study the bacterial communities that are altered as a result of creosote treatment and PAH degradation. The rationale behind our study is to facilitate reconsidering factors leading to bioremediation of creosote contaminated soils. Methodology Large containers filled with either spruce forest soil, pine forest soil, peat or glacial sand were contaminated with creosote or left intact. The degradation of PAHs, primarily fluoranthene, phenanthrene and pyrene, was followed for 27 weeks. The degradation was monitored at 4, 13 and 27 weeks using gas chromatography-mass spectroscopy (GC-MS), and bacterial community composition was analysed using 454 pyrosequencing.Results and Discussion Relative abundance and richness of Proteobacteria, particularly Betaproteobacteria and Deltaproteobacteria, was higher while Bacteroidetes and several genuses under the phylum Bacteroidetes including Sphingobacteriia was less abundant in creosote contaminated soils. This finding could be turn out to be important in bioremediation approach of creosote or PAH contaminated sites.

AB - Objective Long-term exposure to polyaromatic hydrocarbons (PAHs) in living environment has been connected to chronic health disorders in humans. Almost 90% of the PAHs emitted into the environment naturally or as a result of human activity end up in the top 20 cm of the soil. Microbial degradation and abiotic disappearance of PAHs favor certain microbial taxa, while others become less prevalent as a result of resource competition and direct PAH toxicity. Bacterial species that prosper under PAH exposure vary from soil to soil. Assuming that the goal is to understand microbial shifts in PAHs polluted soils, it is necessary to study microbial community changes in several soils concurrently. This study intended to study the bacterial communities that are altered as a result of creosote treatment and PAH degradation. The rationale behind our study is to facilitate reconsidering factors leading to bioremediation of creosote contaminated soils. Methodology Large containers filled with either spruce forest soil, pine forest soil, peat or glacial sand were contaminated with creosote or left intact. The degradation of PAHs, primarily fluoranthene, phenanthrene and pyrene, was followed for 27 weeks. The degradation was monitored at 4, 13 and 27 weeks using gas chromatography-mass spectroscopy (GC-MS), and bacterial community composition was analysed using 454 pyrosequencing.Results and Discussion Relative abundance and richness of Proteobacteria, particularly Betaproteobacteria and Deltaproteobacteria, was higher while Bacteroidetes and several genuses under the phylum Bacteroidetes including Sphingobacteriia was less abundant in creosote contaminated soils. This finding could be turn out to be important in bioremediation approach of creosote or PAH contaminated sites.

KW - 1172 Environmental sciences

KW - Creosote

KW - BACTERIAL COMMUNITIES

KW - Proteobacteria

KW - Bacteroidetes

KW - pristine soil

M3 - Poster

ER -

Parajuli A, Sinkkonen AT, Grönroos KMM. Alteration in Microbial Community in Creosote Contaminated Soils. 2017. Posterin esittämispaikka: 6th Annual International Conference on Sustainable Energy and Environmental Science, Singapore, Singapore.