Altered Bile Transporter Expression and Cholesterol Metabolism in Children With Cholesterol and Pigment Gallstones

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Objectives: We elucidated pathophysiology of pediatric gallstone disease by assessing liver expression of bile transporters in relation to bile acids and surrogates of cholesterol absorption and synthesis in serum and gallstones. Methods: RNA expression of canalicular bile transporters in liver biopsies from 32 pediatric gallstone patients and from 6 liver donors (controls) was measured by qRT-PCR (quantitative real-time reverse transcription polymerase chain reaction). Concentrations of cholesterol and precursors, plant sterols and bile acids in gallstones, and in serum of the patients and 82 healthy children were measured. Primary outcomes were the difference in RNA expressions and serum sterol profiles between patients and controls. Results: Cholesterol stones (CS; n = 15) contained cholesterol >42% and pigment stones (PS; n = 17)

Alkuperäiskielienglanti
LehtiJournal of Pediatric Gastroenterology and Nutrition
Vuosikerta69
Numero2
Sivut138-144
Sivumäärä7
ISSN0277-2116
DOI - pysyväislinkit
TilaJulkaistu - elokuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 3121 Sisätaudit
  • 3123 Naisten- ja lastentaudit

Lainaa tätä

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title = "Altered Bile Transporter Expression and Cholesterol Metabolism in Children With Cholesterol and Pigment Gallstones",
abstract = "Objectives: We elucidated pathophysiology of pediatric gallstone disease by assessing liver expression of bile transporters in relation to bile acids and surrogates of cholesterol absorption and synthesis in serum and gallstones. Methods: RNA expression of canalicular bile transporters in liver biopsies from 32 pediatric gallstone patients and from 6 liver donors (controls) was measured by qRT-PCR (quantitative real-time reverse transcription polymerase chain reaction). Concentrations of cholesterol and precursors, plant sterols and bile acids in gallstones, and in serum of the patients and 82 healthy children were measured. Primary outcomes were the difference in RNA expressions and serum sterol profiles between patients and controls. Results: Cholesterol stones (CS; n = 15) contained cholesterol >42{\%} and pigment stones (PS; n = 17)",
keywords = "3121 Internal medicine, PLANT STEROLS, BILE ACIDS, RNA EXPRESSION, 3123 Gynaecology and paediatrics, bile acids, plant sterols, RNA expression, SURROGATE MARKERS, GALLBLADDER-DISEASE, ABSORPTION, RISK, STEROLS, LIVER, IDENTIFICATION, METAANALYSIS, ABCG5",
author = "Antti Koivusalo and Annika Mutanen and Markku Nissinen and Helena Gylling and Mikko Pakarinen",
year = "2019",
month = "8",
doi = "10.1097/MPG.0000000000002353",
language = "English",
volume = "69",
pages = "138--144",
journal = "Journal of Pediatric Gastroenterology and Nutrition",
issn = "0277-2116",
publisher = "Lippincott williams & wilkins",
number = "2",

}

TY - JOUR

T1 - Altered Bile Transporter Expression and Cholesterol Metabolism in Children With Cholesterol and Pigment Gallstones

AU - Koivusalo, Antti

AU - Mutanen, Annika

AU - Nissinen, Markku

AU - Gylling, Helena

AU - Pakarinen, Mikko

PY - 2019/8

Y1 - 2019/8

N2 - Objectives: We elucidated pathophysiology of pediatric gallstone disease by assessing liver expression of bile transporters in relation to bile acids and surrogates of cholesterol absorption and synthesis in serum and gallstones. Methods: RNA expression of canalicular bile transporters in liver biopsies from 32 pediatric gallstone patients and from 6 liver donors (controls) was measured by qRT-PCR (quantitative real-time reverse transcription polymerase chain reaction). Concentrations of cholesterol and precursors, plant sterols and bile acids in gallstones, and in serum of the patients and 82 healthy children were measured. Primary outcomes were the difference in RNA expressions and serum sterol profiles between patients and controls. Results: Cholesterol stones (CS; n = 15) contained cholesterol >42% and pigment stones (PS; n = 17)

AB - Objectives: We elucidated pathophysiology of pediatric gallstone disease by assessing liver expression of bile transporters in relation to bile acids and surrogates of cholesterol absorption and synthesis in serum and gallstones. Methods: RNA expression of canalicular bile transporters in liver biopsies from 32 pediatric gallstone patients and from 6 liver donors (controls) was measured by qRT-PCR (quantitative real-time reverse transcription polymerase chain reaction). Concentrations of cholesterol and precursors, plant sterols and bile acids in gallstones, and in serum of the patients and 82 healthy children were measured. Primary outcomes were the difference in RNA expressions and serum sterol profiles between patients and controls. Results: Cholesterol stones (CS; n = 15) contained cholesterol >42% and pigment stones (PS; n = 17)

KW - 3121 Internal medicine

KW - PLANT STEROLS

KW - BILE ACIDS

KW - RNA EXPRESSION

KW - 3123 Gynaecology and paediatrics

KW - bile acids

KW - plant sterols

KW - RNA expression

KW - SURROGATE MARKERS

KW - GALLBLADDER-DISEASE

KW - ABSORPTION

KW - RISK

KW - STEROLS

KW - LIVER

KW - IDENTIFICATION

KW - METAANALYSIS

KW - ABCG5

U2 - 10.1097/MPG.0000000000002353

DO - 10.1097/MPG.0000000000002353

M3 - Article

VL - 69

SP - 138

EP - 144

JO - Journal of Pediatric Gastroenterology and Nutrition

JF - Journal of Pediatric Gastroenterology and Nutrition

SN - 0277-2116

IS - 2

ER -