Antibody response in snakes with boid inclusion body disease

Katharina Windbichler, Eleni Michalopoulou, Pia Palamides, Theresa Pesch, Christine Jelinek, Olli Vapalahti, Anja Kipar, Udo Hetzel, Jussi Hepojoki

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Boid Inclusion Body Disease (BIBD) is a potentially fatal disease reported in captive boid snakes worldwide that is caused by reptarenavirus infection. Although the detection of intracytoplasmic inclusion bodies (IB) in blood cells serves as the gold standard for the ante mortem diagnosis of BIBD, the mechanisms underlying IB formation and the pathogenesis of BIBD are unknown. Knowledge on the reptile immune system is sparse compared to the mammalian counterpart, and in particular the response towards reptarenavirus infection is practically unknown. Herein, we investigated a breeding collection of 70 Boa constrictor snakes for BIBD, reptarenavirus viraemia, anti-reptarenavirus IgM and IgY antibodies, and population parameters. Using NGS and RT-PCR on pooled blood samples of snakes with and without BIBD, we could identify three different reptarenavirus S segments in the collection. The examination of individual samples by RT-PCR indicated that the presence of University of Giessen virus (UGV)-like S segment strongly correlates with IB formation. We could also demonstrate a negative correlation between BIBD and the presence of anti-UGV NP IgY antibodies. Further evidence of an association between antibody response and BIBD is the finding that the level of anti-reptarenavirus antibodies measured by ELISA was lower in snakes with BIBD. Furthermore, female snakes had a significantly lower body weight when they had BIBD. Taken together our findings suggest that the detection of the UGV-/S6-like S segment and the presence of anti-reptarenavirus IgY antibodies might serve as a prognostic tool for predicting the development of BIBD.
Alkuperäiskielienglanti
LehtiPLoS One
Vuosikerta14
Numero9
Sivute0221863
ISSN1932-6203
DOI - pysyväislinkit
TilaJulkaistu - 9 syyskuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 413 Eläinlääketiede

Lainaa tätä

Windbichler, K., Michalopoulou, E., Palamides, P., Pesch, T., Jelinek, C., Vapalahti, O., ... Hepojoki, J. (2019). Antibody response in snakes with boid inclusion body disease. PLoS One, 14(9), e0221863. https://doi.org/10.1371/journal.pone.0221863
Windbichler, Katharina ; Michalopoulou, Eleni ; Palamides, Pia ; Pesch, Theresa ; Jelinek, Christine ; Vapalahti, Olli ; Kipar, Anja ; Hetzel, Udo ; Hepojoki, Jussi. / Antibody response in snakes with boid inclusion body disease. Julkaisussa: PLoS One. 2019 ; Vuosikerta 14, Nro 9. Sivut e0221863.
@article{5eebd2c534744f429ba1601a84289081,
title = "Antibody response in snakes with boid inclusion body disease",
abstract = "Boid Inclusion Body Disease (BIBD) is a potentially fatal disease reported in captive boid snakes worldwide that is caused by reptarenavirus infection. Although the detection of intracytoplasmic inclusion bodies (IB) in blood cells serves as the gold standard for the ante mortem diagnosis of BIBD, the mechanisms underlying IB formation and the pathogenesis of BIBD are unknown. Knowledge on the reptile immune system is sparse compared to the mammalian counterpart, and in particular the response towards reptarenavirus infection is practically unknown. Herein, we investigated a breeding collection of 70 Boa constrictor snakes for BIBD, reptarenavirus viraemia, anti-reptarenavirus IgM and IgY antibodies, and population parameters. Using NGS and RT-PCR on pooled blood samples of snakes with and without BIBD, we could identify three different reptarenavirus S segments in the collection. The examination of individual samples by RT-PCR indicated that the presence of University of Giessen virus (UGV)-like S segment strongly correlates with IB formation. We could also demonstrate a negative correlation between BIBD and the presence of anti-UGV NP IgY antibodies. Further evidence of an association between antibody response and BIBD is the finding that the level of anti-reptarenavirus antibodies measured by ELISA was lower in snakes with BIBD. Furthermore, female snakes had a significantly lower body weight when they had BIBD. Taken together our findings suggest that the detection of the UGV-/S6-like S segment and the presence of anti-reptarenavirus IgY antibodies might serve as a prognostic tool for predicting the development of BIBD.",
keywords = "413 Veterinary science",
author = "Katharina Windbichler and Eleni Michalopoulou and Pia Palamides and Theresa Pesch and Christine Jelinek and Olli Vapalahti and Anja Kipar and Udo Hetzel and Jussi Hepojoki",
year = "2019",
month = "9",
day = "9",
doi = "10.1371/journal.pone.0221863",
language = "English",
volume = "14",
pages = "e0221863",
journal = "PLoS One",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY OF SCIENCE",
number = "9",

}

Windbichler, K, Michalopoulou, E, Palamides, P, Pesch, T, Jelinek, C, Vapalahti, O, Kipar, A, Hetzel, U & Hepojoki, J 2019, 'Antibody response in snakes with boid inclusion body disease', PLoS One, Vuosikerta 14, Nro 9, Sivut e0221863. https://doi.org/10.1371/journal.pone.0221863

Antibody response in snakes with boid inclusion body disease. / Windbichler, Katharina; Michalopoulou, Eleni; Palamides, Pia; Pesch, Theresa; Jelinek, Christine; Vapalahti, Olli; Kipar, Anja; Hetzel, Udo; Hepojoki, Jussi.

julkaisussa: PLoS One, Vuosikerta 14, Nro 9, 09.09.2019, s. e0221863.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Antibody response in snakes with boid inclusion body disease

AU - Windbichler, Katharina

AU - Michalopoulou, Eleni

AU - Palamides, Pia

AU - Pesch, Theresa

AU - Jelinek, Christine

AU - Vapalahti, Olli

AU - Kipar, Anja

AU - Hetzel, Udo

AU - Hepojoki, Jussi

PY - 2019/9/9

Y1 - 2019/9/9

N2 - Boid Inclusion Body Disease (BIBD) is a potentially fatal disease reported in captive boid snakes worldwide that is caused by reptarenavirus infection. Although the detection of intracytoplasmic inclusion bodies (IB) in blood cells serves as the gold standard for the ante mortem diagnosis of BIBD, the mechanisms underlying IB formation and the pathogenesis of BIBD are unknown. Knowledge on the reptile immune system is sparse compared to the mammalian counterpart, and in particular the response towards reptarenavirus infection is practically unknown. Herein, we investigated a breeding collection of 70 Boa constrictor snakes for BIBD, reptarenavirus viraemia, anti-reptarenavirus IgM and IgY antibodies, and population parameters. Using NGS and RT-PCR on pooled blood samples of snakes with and without BIBD, we could identify three different reptarenavirus S segments in the collection. The examination of individual samples by RT-PCR indicated that the presence of University of Giessen virus (UGV)-like S segment strongly correlates with IB formation. We could also demonstrate a negative correlation between BIBD and the presence of anti-UGV NP IgY antibodies. Further evidence of an association between antibody response and BIBD is the finding that the level of anti-reptarenavirus antibodies measured by ELISA was lower in snakes with BIBD. Furthermore, female snakes had a significantly lower body weight when they had BIBD. Taken together our findings suggest that the detection of the UGV-/S6-like S segment and the presence of anti-reptarenavirus IgY antibodies might serve as a prognostic tool for predicting the development of BIBD.

AB - Boid Inclusion Body Disease (BIBD) is a potentially fatal disease reported in captive boid snakes worldwide that is caused by reptarenavirus infection. Although the detection of intracytoplasmic inclusion bodies (IB) in blood cells serves as the gold standard for the ante mortem diagnosis of BIBD, the mechanisms underlying IB formation and the pathogenesis of BIBD are unknown. Knowledge on the reptile immune system is sparse compared to the mammalian counterpart, and in particular the response towards reptarenavirus infection is practically unknown. Herein, we investigated a breeding collection of 70 Boa constrictor snakes for BIBD, reptarenavirus viraemia, anti-reptarenavirus IgM and IgY antibodies, and population parameters. Using NGS and RT-PCR on pooled blood samples of snakes with and without BIBD, we could identify three different reptarenavirus S segments in the collection. The examination of individual samples by RT-PCR indicated that the presence of University of Giessen virus (UGV)-like S segment strongly correlates with IB formation. We could also demonstrate a negative correlation between BIBD and the presence of anti-UGV NP IgY antibodies. Further evidence of an association between antibody response and BIBD is the finding that the level of anti-reptarenavirus antibodies measured by ELISA was lower in snakes with BIBD. Furthermore, female snakes had a significantly lower body weight when they had BIBD. Taken together our findings suggest that the detection of the UGV-/S6-like S segment and the presence of anti-reptarenavirus IgY antibodies might serve as a prognostic tool for predicting the development of BIBD.

KW - 413 Veterinary science

U2 - 10.1371/journal.pone.0221863

DO - 10.1371/journal.pone.0221863

M3 - Article

VL - 14

SP - e0221863

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

ER -

Windbichler K, Michalopoulou E, Palamides P, Pesch T, Jelinek C, Vapalahti O et al. Antibody response in snakes with boid inclusion body disease. PLoS One. 2019 syys 9;14(9):e0221863. https://doi.org/10.1371/journal.pone.0221863