Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

Kamand  Tavakoli; Alireza Pour-Aboughadareh; Farzad  Kianersi; Péter Poczai; Alireza  Etminan; Lia Shooshtari

doi:10.3390/biotech10030014

Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

Kamand Tavakoli
, Alireza Pour-Aboughadareh
, Farzad Kianersi
, Péter Poczai
, Alireza Etminan
, Lia Shooshtari

Tutkimustuotos: Artikkelijulkaisu › Artikkeli › Tieteellinen › vertaisarvioitu

Abstrakti

Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.

Alkuperäiskieli	englanti
Artikkeli	14
Lehti	BioTech
Vuosikerta	10
Numero	3
ISSN	2673-6284
DOI - pysyväislinkit	https://doi.org/10.3390/biotech10030014
Tila	Julkaistu - 2021
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

1184 Genetiikka, kehitysbiologia, fysiologia
414 Maatalouden bioteknologia
318 Lääketieteen bioteknologia

Pääsy asiakirjaan

10.3390/biotech10030014

biotech-10-00014-v2Lopullinen julkaistu versio, 3 MBLisenssi: CC BY
CRISPR_CAS_BioTech_2021Lopullinen julkaistu versio, 3 MBLisenssi: CC BY

1 Päättynyt

PLAGE: Genetic diversity, conservation and utilization of plant genetic resources
Poczai, P. (Principal Investigator), Amiryousefi, A. (Osallistuja), Cseh, A. (Principal Investigator), Karsai, I. (Principal Investigator), Balla, K. (Osallistuja), Kiss, T. (Osallistuja), Bányai, J. (Osallistuja) & Illés, K. (Osallistuja)
01/09/2017 → 31/12/2022
Projekti: Tutkimusprojekti

Kansallinen elektroninen kirjasto FinELib
Helsingin yliopisto
Laitteistot/tilat: External collaboration
Lajitietokeskus
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Laitteistot/tilat: Keskuspalveluyksikkö
Luonnontieteellinen keskusmuseo ja sen kokoelmat
Luonnontieteellinen keskusmuseo
Laitteistot/tilat: Host unit

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title = "Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences",

abstract = "Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.",

keywords = "1184 Genetics, developmental biology, physiology, 414 Agricultural biotechnology, 318 Medical biotechnology",

author = "Kamand Tavakoli and Alireza Pour-Aboughadareh and Farzad Kianersi and P{\'e}ter Poczai and Alireza Etminan and Lia Shooshtari",

year = "2021",

doi = "10.3390/biotech10030014",

language = "English",

volume = "10",

journal = "BioTech",

issn = "2673-6284",

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TY - JOUR

T1 - Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

AU - Tavakoli, Kamand

AU - Pour-Aboughadareh, Alireza

AU - Kianersi, Farzad

AU - Poczai, Péter

AU - Etminan, Alireza

AU - Shooshtari, Lia

PY - 2021

Y1 - 2021

N2 - Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.

AB - Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.

KW - 1184 Genetics, developmental biology, physiology

KW - 414 Agricultural biotechnology

KW - 318 Medical biotechnology

U2 - 10.3390/biotech10030014

DO - 10.3390/biotech10030014

M3 - Article

SN - 2673-6284

VL - 10

JO - BioTech

JF - BioTech

IS - 3

M1 - 14

ER -

Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

Abstrakti

Tieteenalat

Pääsy asiakirjaan

Projektit

PLAGE: Genetic diversity, conservation and utilization of plant genetic resources

Laitteet

Kansallinen elektroninen kirjasto FinELib

Lajitietokeskus

Luonnontieteellinen keskusmuseo ja sen kokoelmat

Siteeraa tätä