Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species

Fuqiang Cui, Hongpo Wu, Omid Safronov, Panpan Zhang, Rajeev Kumar, Hannes Kollist, Jarkko Tapani Salojärvi, Ralph Panstruga, Kirk Loren Overmyer

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

The atmospheric pollutant ozone (O-3) is a strong oxidant that causes extracellular reactive oxygen species (ROS) formation, has significant ecological relevance, and is used here as a non-invasive ROS inducer to study plant signalling. Previous genetic screens identified several mutants exhibiting enhanced O-3 sensitivity, but few with enhanced tolerance. We found that loss-of-function mutants in Arabidopsis MLO2, a gene implicated in susceptibility to powdery mildew disease, exhibit enhanced dose-dependent tolerance to O-3 and extracellular ROS, but a normal response to intracellular ROS. This phenotype is increased in a mlo2 mlo6 mlo12 triple mutant, reminiscent of the genetic redundancy of MLO genes in powdery mildew resistance. Stomatal assays revealed that enhanced O-3 tolerance in mlo2 mutants is not caused by altered stomatal conductance. We explored modulation of the mlo2-associated O-3 tolerance, powdery mildew resistance, and early senescence phenotypes by genetic epistasis analysis, involving mutants with known effects on ROS sensitivity or antifungal defence. Mining of publicly accessible microarray data suggests that these MLO proteins regulate accumulation of abiotic stress response transcripts, and transcript accumulation of MLO2 itself is O-3 responsive. In summary, our data reveal MLO2 as a novel negative regulator in plant ROS responses, which links biotic and abiotic stress response pathways.

Alkuperäiskielienglanti
LehtiPlant, Cell and Environment
Vuosikerta41
Numero4
Sivut782-796
Sivumäärä15
ISSN1365-3040
DOI - pysyväislinkit
TilaJulkaistu - huhtikuuta 2018
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1183 Kasvibiologia, mikrobiologia, virologia

Lainaa tätä

Cui, Fuqiang ; Wu, Hongpo ; Safronov, Omid ; Zhang, Panpan ; Kumar, Rajeev ; Kollist, Hannes ; Salojärvi, Jarkko Tapani ; Panstruga, Ralph ; Overmyer, Kirk Loren. / Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species. Julkaisussa: Plant, Cell and Environment. 2018 ; Vuosikerta 41, Nro 4. Sivut 782-796.
@article{ffe4233c508b4cb583c9f13b7b6d245a,
title = "Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species",
abstract = "The atmospheric pollutant ozone (O-3) is a strong oxidant that causes extracellular reactive oxygen species (ROS) formation, has significant ecological relevance, and is used here as a non-invasive ROS inducer to study plant signalling. Previous genetic screens identified several mutants exhibiting enhanced O-3 sensitivity, but few with enhanced tolerance. We found that loss-of-function mutants in Arabidopsis MLO2, a gene implicated in susceptibility to powdery mildew disease, exhibit enhanced dose-dependent tolerance to O-3 and extracellular ROS, but a normal response to intracellular ROS. This phenotype is increased in a mlo2 mlo6 mlo12 triple mutant, reminiscent of the genetic redundancy of MLO genes in powdery mildew resistance. Stomatal assays revealed that enhanced O-3 tolerance in mlo2 mutants is not caused by altered stomatal conductance. We explored modulation of the mlo2-associated O-3 tolerance, powdery mildew resistance, and early senescence phenotypes by genetic epistasis analysis, involving mutants with known effects on ROS sensitivity or antifungal defence. Mining of publicly accessible microarray data suggests that these MLO proteins regulate accumulation of abiotic stress response transcripts, and transcript accumulation of MLO2 itself is O-3 responsive. In summary, our data reveal MLO2 as a novel negative regulator in plant ROS responses, which links biotic and abiotic stress response pathways.",
keywords = "1183 Plant biology, microbiology, virology, abiotic stress, immunity, ozone, ROS signalling, unequal genetic redundancy, RESPONSIVE GENE-EXPRESSION, POWDERY MILDEW, CELL-DEATH, SALICYLIC-ACID, DISEASE RESISTANCE, NONHOST RESISTANCE, OZONE SENSITIVITY, ANTIFUNGAL DEFENSE, SIGNALING PATHWAYS, STOMATAL RESPONSE",
author = "Fuqiang Cui and Hongpo Wu and Omid Safronov and Panpan Zhang and Rajeev Kumar and Hannes Kollist and Saloj{\"a}rvi, {Jarkko Tapani} and Ralph Panstruga and Overmyer, {Kirk Loren}",
year = "2018",
month = "4",
doi = "10.1111/pce.13144",
language = "English",
volume = "41",
pages = "782--796",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley",
number = "4",

}

Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species. / Cui, Fuqiang; Wu, Hongpo; Safronov, Omid; Zhang, Panpan; Kumar, Rajeev; Kollist, Hannes; Salojärvi, Jarkko Tapani; Panstruga, Ralph; Overmyer, Kirk Loren.

julkaisussa: Plant, Cell and Environment, Vuosikerta 41, Nro 4, 04.2018, s. 782-796.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species

AU - Cui, Fuqiang

AU - Wu, Hongpo

AU - Safronov, Omid

AU - Zhang, Panpan

AU - Kumar, Rajeev

AU - Kollist, Hannes

AU - Salojärvi, Jarkko Tapani

AU - Panstruga, Ralph

AU - Overmyer, Kirk Loren

PY - 2018/4

Y1 - 2018/4

N2 - The atmospheric pollutant ozone (O-3) is a strong oxidant that causes extracellular reactive oxygen species (ROS) formation, has significant ecological relevance, and is used here as a non-invasive ROS inducer to study plant signalling. Previous genetic screens identified several mutants exhibiting enhanced O-3 sensitivity, but few with enhanced tolerance. We found that loss-of-function mutants in Arabidopsis MLO2, a gene implicated in susceptibility to powdery mildew disease, exhibit enhanced dose-dependent tolerance to O-3 and extracellular ROS, but a normal response to intracellular ROS. This phenotype is increased in a mlo2 mlo6 mlo12 triple mutant, reminiscent of the genetic redundancy of MLO genes in powdery mildew resistance. Stomatal assays revealed that enhanced O-3 tolerance in mlo2 mutants is not caused by altered stomatal conductance. We explored modulation of the mlo2-associated O-3 tolerance, powdery mildew resistance, and early senescence phenotypes by genetic epistasis analysis, involving mutants with known effects on ROS sensitivity or antifungal defence. Mining of publicly accessible microarray data suggests that these MLO proteins regulate accumulation of abiotic stress response transcripts, and transcript accumulation of MLO2 itself is O-3 responsive. In summary, our data reveal MLO2 as a novel negative regulator in plant ROS responses, which links biotic and abiotic stress response pathways.

AB - The atmospheric pollutant ozone (O-3) is a strong oxidant that causes extracellular reactive oxygen species (ROS) formation, has significant ecological relevance, and is used here as a non-invasive ROS inducer to study plant signalling. Previous genetic screens identified several mutants exhibiting enhanced O-3 sensitivity, but few with enhanced tolerance. We found that loss-of-function mutants in Arabidopsis MLO2, a gene implicated in susceptibility to powdery mildew disease, exhibit enhanced dose-dependent tolerance to O-3 and extracellular ROS, but a normal response to intracellular ROS. This phenotype is increased in a mlo2 mlo6 mlo12 triple mutant, reminiscent of the genetic redundancy of MLO genes in powdery mildew resistance. Stomatal assays revealed that enhanced O-3 tolerance in mlo2 mutants is not caused by altered stomatal conductance. We explored modulation of the mlo2-associated O-3 tolerance, powdery mildew resistance, and early senescence phenotypes by genetic epistasis analysis, involving mutants with known effects on ROS sensitivity or antifungal defence. Mining of publicly accessible microarray data suggests that these MLO proteins regulate accumulation of abiotic stress response transcripts, and transcript accumulation of MLO2 itself is O-3 responsive. In summary, our data reveal MLO2 as a novel negative regulator in plant ROS responses, which links biotic and abiotic stress response pathways.

KW - 1183 Plant biology, microbiology, virology

KW - abiotic stress

KW - immunity

KW - ozone

KW - ROS signalling

KW - unequal genetic redundancy

KW - RESPONSIVE GENE-EXPRESSION

KW - POWDERY MILDEW

KW - CELL-DEATH

KW - SALICYLIC-ACID

KW - DISEASE RESISTANCE

KW - NONHOST RESISTANCE

KW - OZONE SENSITIVITY

KW - ANTIFUNGAL DEFENSE

KW - SIGNALING PATHWAYS

KW - STOMATAL RESPONSE

U2 - 10.1111/pce.13144

DO - 10.1111/pce.13144

M3 - Article

VL - 41

SP - 782

EP - 796

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 4

ER -