Archaeal rRNA diversity and methane production in deep boreal peat

Anuliina Putkinen, Heli Juottonen, Sari Juutinen, Eeva-Stiina Tuittila, Hannu Fritze, Kim Yrjälä

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Northern peatlands play a major role in the global carbon cycle as sinks for CO2 and as sources of CH4. These diverse ecosystems develop through accumulation of partially decomposed plant material as peat. With increasing depth, peat becomes more and more recalcitrant due to its longer exposure to decomposing processes. Compared with surface peat, deeper peat sediments remain microbiologically poorly described. We detected active archaeal communities even in the deep bottom layers (-220/-280 cm) of two Finnish fen-type peatlands by 16S rRNA-based terminal restriction fragment length polymorphism analysis. In the sediments of the northern study site, all detected archaea were methanogens with Rice Cluster II (RC-II) and Methanosaetaceae as major groups. In southern peatland, Crenarchaeota of a rare unidentified cluster were present together with mainly RC-II methanogens. RNA profiles showed a larger archaeal diversity than DNA-based community profiles, suggesting that small but active populations were better visualized with rRNA. In addition, potential methane production measurements indicated methanogenic activity throughout the vertical peat profiles.
Alkuperäiskielienglanti
LehtiFEMS Microbiology Ecology
Vuosikerta70
Numero1
Sivut87-98
Sivumäärä12
ISSN0168-6496
DOI - pysyväislinkit
TilaJulkaistu - 2009
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 411 Maatalous ja metsätieteet

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Putkinen, Anuliina ; Juottonen, Heli ; Juutinen, Sari ; Tuittila, Eeva-Stiina ; Fritze, Hannu ; Yrjälä, Kim. / Archaeal rRNA diversity and methane production in deep boreal peat. Julkaisussa: FEMS Microbiology Ecology. 2009 ; Vuosikerta 70, Nro 1. Sivut 87-98.
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title = "Archaeal rRNA diversity and methane production in deep boreal peat",
abstract = "Northern peatlands play a major role in the global carbon cycle as sinks for CO2 and as sources of CH4. These diverse ecosystems develop through accumulation of partially decomposed plant material as peat. With increasing depth, peat becomes more and more recalcitrant due to its longer exposure to decomposing processes. Compared with surface peat, deeper peat sediments remain microbiologically poorly described. We detected active archaeal communities even in the deep bottom layers (-220/-280 cm) of two Finnish fen-type peatlands by 16S rRNA-based terminal restriction fragment length polymorphism analysis. In the sediments of the northern study site, all detected archaea were methanogens with Rice Cluster II (RC-II) and Methanosaetaceae as major groups. In southern peatland, Crenarchaeota of a rare unidentified cluster were present together with mainly RC-II methanogens. RNA profiles showed a larger archaeal diversity than DNA-based community profiles, suggesting that small but active populations were better visualized with rRNA. In addition, potential methane production measurements indicated methanogenic activity throughout the vertical peat profiles.",
keywords = "411 Agriculture and forestry",
author = "Anuliina Putkinen and Heli Juottonen and Sari Juutinen and Eeva-Stiina Tuittila and Hannu Fritze and Kim Yrj{\"a}l{\"a}",
year = "2009",
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pages = "87--98",
journal = "FEMS Microbiology Ecology",
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Archaeal rRNA diversity and methane production in deep boreal peat. / Putkinen, Anuliina; Juottonen, Heli; Juutinen, Sari; Tuittila, Eeva-Stiina; Fritze, Hannu; Yrjälä, Kim.

julkaisussa: FEMS Microbiology Ecology, Vuosikerta 70, Nro 1, 2009, s. 87-98.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Archaeal rRNA diversity and methane production in deep boreal peat

AU - Putkinen, Anuliina

AU - Juottonen, Heli

AU - Juutinen, Sari

AU - Tuittila, Eeva-Stiina

AU - Fritze, Hannu

AU - Yrjälä, Kim

PY - 2009

Y1 - 2009

N2 - Northern peatlands play a major role in the global carbon cycle as sinks for CO2 and as sources of CH4. These diverse ecosystems develop through accumulation of partially decomposed plant material as peat. With increasing depth, peat becomes more and more recalcitrant due to its longer exposure to decomposing processes. Compared with surface peat, deeper peat sediments remain microbiologically poorly described. We detected active archaeal communities even in the deep bottom layers (-220/-280 cm) of two Finnish fen-type peatlands by 16S rRNA-based terminal restriction fragment length polymorphism analysis. In the sediments of the northern study site, all detected archaea were methanogens with Rice Cluster II (RC-II) and Methanosaetaceae as major groups. In southern peatland, Crenarchaeota of a rare unidentified cluster were present together with mainly RC-II methanogens. RNA profiles showed a larger archaeal diversity than DNA-based community profiles, suggesting that small but active populations were better visualized with rRNA. In addition, potential methane production measurements indicated methanogenic activity throughout the vertical peat profiles.

AB - Northern peatlands play a major role in the global carbon cycle as sinks for CO2 and as sources of CH4. These diverse ecosystems develop through accumulation of partially decomposed plant material as peat. With increasing depth, peat becomes more and more recalcitrant due to its longer exposure to decomposing processes. Compared with surface peat, deeper peat sediments remain microbiologically poorly described. We detected active archaeal communities even in the deep bottom layers (-220/-280 cm) of two Finnish fen-type peatlands by 16S rRNA-based terminal restriction fragment length polymorphism analysis. In the sediments of the northern study site, all detected archaea were methanogens with Rice Cluster II (RC-II) and Methanosaetaceae as major groups. In southern peatland, Crenarchaeota of a rare unidentified cluster were present together with mainly RC-II methanogens. RNA profiles showed a larger archaeal diversity than DNA-based community profiles, suggesting that small but active populations were better visualized with rRNA. In addition, potential methane production measurements indicated methanogenic activity throughout the vertical peat profiles.

KW - 411 Agriculture and forestry

U2 - 10.1111/j.1574-6941.2009.00738.x

DO - 10.1111/j.1574-6941.2009.00738.x

M3 - Article

VL - 70

SP - 87

EP - 98

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 1

ER -