BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organisms

Maarit Hölttä-Vuori, Riikka-Liisa Uronen, Jarmila Repakova, Emppu Salonen, Ilpo Vattulainen, Pertti Panula, Zaiguo Li, Robert Bittman, Elina Ikonen

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. In this study, we employed atomistic simulations and experiments to characterize a cholesterol compound with fluorescent boron dipyrromethene difluoride linked to sterol carbon-24 (BODIPY-cholesterol). This probe packed in the membrane and behaved similarly to cholesterol both in normal and in cholesterol-storage disease cells and with trace amounts allowed the visualization of sterol movement in living systems. Upon injection into the yolk sac, BODIPY-cholesterol did not disturb zebrafish development and was targeted to sterol-enriched brain regions in live fish. We conclude that this new probe closely mimics the membrane partitioning and trafficking of cholesterol and, because of its excellent fluorescent properties, enables the direct monitoring of sterol movement by time-lapse imaging using trace amounts of the probe. This is, to our knowledge, the first cholesterol probe that fulfills these prerequisites.
Alkuperäiskielienglanti
LehtiTraffic
Vuosikerta9
Numero11
Sivut1839-1849
Sivumäärä11
ISSN1600-0854
DOI - pysyväislinkit
TilaJulkaistu - 2008
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 311 Peruslääketieteet
  • 118 Biotieteet
  • 114 Fysiikka
  • 515 Psykologia

Lainaa tätä

Hölttä-Vuori, Maarit ; Uronen, Riikka-Liisa ; Repakova, Jarmila ; Salonen, Emppu ; Vattulainen, Ilpo ; Panula, Pertti ; Li, Zaiguo ; Bittman, Robert ; Ikonen, Elina. / BODIPY-cholesterol : a new tool to visualize sterol trafficking in living cells and organisms. Julkaisussa: Traffic. 2008 ; Vuosikerta 9, Nro 11. Sivut 1839-1849.
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title = "BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organisms",
abstract = "Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. In this study, we employed atomistic simulations and experiments to characterize a cholesterol compound with fluorescent boron dipyrromethene difluoride linked to sterol carbon-24 (BODIPY-cholesterol). This probe packed in the membrane and behaved similarly to cholesterol both in normal and in cholesterol-storage disease cells and with trace amounts allowed the visualization of sterol movement in living systems. Upon injection into the yolk sac, BODIPY-cholesterol did not disturb zebrafish development and was targeted to sterol-enriched brain regions in live fish. We conclude that this new probe closely mimics the membrane partitioning and trafficking of cholesterol and, because of its excellent fluorescent properties, enables the direct monitoring of sterol movement by time-lapse imaging using trace amounts of the probe. This is, to our knowledge, the first cholesterol probe that fulfills these prerequisites.",
keywords = "311 Basic medicine, 118 Biological sciences, 114 Physical sciences, 515 Psychology",
author = "Maarit H{\"o}ltt{\"a}-Vuori and Riikka-Liisa Uronen and Jarmila Repakova and Emppu Salonen and Ilpo Vattulainen and Pertti Panula and Zaiguo Li and Robert Bittman and Elina Ikonen",
year = "2008",
doi = "10.1111/j.1600-0854.2008.00801.x",
language = "English",
volume = "9",
pages = "1839--1849",
journal = "Traffic",
issn = "1600-0854",
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BODIPY-cholesterol : a new tool to visualize sterol trafficking in living cells and organisms. / Hölttä-Vuori, Maarit; Uronen, Riikka-Liisa; Repakova, Jarmila; Salonen, Emppu; Vattulainen, Ilpo; Panula, Pertti; Li, Zaiguo; Bittman, Robert; Ikonen, Elina.

julkaisussa: Traffic, Vuosikerta 9, Nro 11, 2008, s. 1839-1849.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - BODIPY-cholesterol

T2 - a new tool to visualize sterol trafficking in living cells and organisms

AU - Hölttä-Vuori, Maarit

AU - Uronen, Riikka-Liisa

AU - Repakova, Jarmila

AU - Salonen, Emppu

AU - Vattulainen, Ilpo

AU - Panula, Pertti

AU - Li, Zaiguo

AU - Bittman, Robert

AU - Ikonen, Elina

PY - 2008

Y1 - 2008

N2 - Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. In this study, we employed atomistic simulations and experiments to characterize a cholesterol compound with fluorescent boron dipyrromethene difluoride linked to sterol carbon-24 (BODIPY-cholesterol). This probe packed in the membrane and behaved similarly to cholesterol both in normal and in cholesterol-storage disease cells and with trace amounts allowed the visualization of sterol movement in living systems. Upon injection into the yolk sac, BODIPY-cholesterol did not disturb zebrafish development and was targeted to sterol-enriched brain regions in live fish. We conclude that this new probe closely mimics the membrane partitioning and trafficking of cholesterol and, because of its excellent fluorescent properties, enables the direct monitoring of sterol movement by time-lapse imaging using trace amounts of the probe. This is, to our knowledge, the first cholesterol probe that fulfills these prerequisites.

AB - Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. In this study, we employed atomistic simulations and experiments to characterize a cholesterol compound with fluorescent boron dipyrromethene difluoride linked to sterol carbon-24 (BODIPY-cholesterol). This probe packed in the membrane and behaved similarly to cholesterol both in normal and in cholesterol-storage disease cells and with trace amounts allowed the visualization of sterol movement in living systems. Upon injection into the yolk sac, BODIPY-cholesterol did not disturb zebrafish development and was targeted to sterol-enriched brain regions in live fish. We conclude that this new probe closely mimics the membrane partitioning and trafficking of cholesterol and, because of its excellent fluorescent properties, enables the direct monitoring of sterol movement by time-lapse imaging using trace amounts of the probe. This is, to our knowledge, the first cholesterol probe that fulfills these prerequisites.

KW - 311 Basic medicine

KW - 118 Biological sciences

KW - 114 Physical sciences

KW - 515 Psychology

U2 - 10.1111/j.1600-0854.2008.00801.x

DO - 10.1111/j.1600-0854.2008.00801.x

M3 - Article

VL - 9

SP - 1839

EP - 1849

JO - Traffic

JF - Traffic

SN - 1600-0854

IS - 11

ER -