Caracterización molecular de células progenitoras del parénquima subcortical cerebral humano adulto

Objective: characterize the behavior of progenitor cells isolated from subcortical parenchyma of human brain in culture. We have analyzed the changes in expression patterns of differentiation/undifferentiation markers as well as cell morphology along the passages and evaluated the potential to be further used as oligodendrocyte progenitors source.

Material and Methods: We isolated progenitor cells from two different samples of subcortical parenchyma human brain of patients suffering from epilepsy. Cells were kept in culture until they became quiescent/senescent. Every other passages RNAs were isolated and checked for the expression of differentiation and undifferentiation markers by using RT-PCR. Proliferation was also addressed by RT-PCR and immunocytochemistry. We carried out cell morphology studies on semithin and ultrathin sections of cells.

Results: We observed decreasing proliferative capacity of both two cell lines which became quiescent/senescent around passages 8-10. We detected the expression of either undifferentiation or early neural and oligodendrocytes differentiation markers.

Conclusions: As for the expression of molecular markers, progenitor cells isolated from subcortical parenchyma of human brain have the potential to differentiate into mature oligodendrocytes