Cell-Nanoparticle Interactions at (Sub)-Nanometer Resolution Analyzed by Electron Microscopy and Correlative Coherent Anti-Stokes Raman Scattering

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

A wide variety of nanoparticles are playing an increasingly important role in drug delivery. Label-free imaging techniques are especially desirable to follow the cellular uptake and intracellular fate of nanoparticles. The combined correlative use of different techniques, each with unique advantages, facilitates more detailed investigation about such interactions. The synergistic use of correlative coherent anti-Stokes Raman scattering and electron microscopy (C-CARS-EM) imaging offers label-free, chemically-specific, and (sub)-nanometer spatial resolution for studying nanoparticle uptake into cells as demonstrated in the current study. Coherent anti-Stokes Raman scattering (CARS) microscopy offers chemically-specific (sub)micron spatial resolution imaging without fluorescent labels while transmission electron microscopy (TEM) offers (sub)-nanometer scale spatial resolution and thus visualization of precise nanoparticle localization at the sub-cellular level. This proof-of-concept imaging platform with unlabeled drug nanocrystals and macrophage cells revealed good colocalization between the CARS signal and electron dense nanocrystals in TEM images. The correlative TEM images revealed subcellular localization of nanocrystals inside membrane bound vesicles, showing multivesicular body (MVB)-like morphology typical for late endosomes (LEs), endolysosomes, and phagolysosomes. C-CARS-EM imaging has much potential to study the interactions between a wide range of nanoparticles and cells with high precision and confidence.

Alkuperäiskielienglanti
Artikkeli1800413
LehtiBiotechnology Journal
Vuosikerta14
Numero4
Sivumäärä10
ISSN1860-6768
DOI - pysyväislinkit
TilaJulkaistu - huhtikuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1182 Biokemia, solu- ja molekyylibiologia
  • 116 Kemia
  • 317 Farmasia

Lainaa tätä

@article{dcc5369f17aa4466a0170f8939705129,
title = "Cell-Nanoparticle Interactions at (Sub)-Nanometer Resolution Analyzed by Electron Microscopy and Correlative Coherent Anti-Stokes Raman Scattering",
abstract = "A wide variety of nanoparticles are playing an increasingly important role in drug delivery. Label-free imaging techniques are especially desirable to follow the cellular uptake and intracellular fate of nanoparticles. The combined correlative use of different techniques, each with unique advantages, facilitates more detailed investigation about such interactions. The synergistic use of correlative coherent anti-Stokes Raman scattering and electron microscopy (C-CARS-EM) imaging offers label-free, chemically-specific, and (sub)-nanometer spatial resolution for studying nanoparticle uptake into cells as demonstrated in the current study. Coherent anti-Stokes Raman scattering (CARS) microscopy offers chemically-specific (sub)micron spatial resolution imaging without fluorescent labels while transmission electron microscopy (TEM) offers (sub)-nanometer scale spatial resolution and thus visualization of precise nanoparticle localization at the sub-cellular level. This proof-of-concept imaging platform with unlabeled drug nanocrystals and macrophage cells revealed good colocalization between the CARS signal and electron dense nanocrystals in TEM images. The correlative TEM images revealed subcellular localization of nanocrystals inside membrane bound vesicles, showing multivesicular body (MVB)-like morphology typical for late endosomes (LEs), endolysosomes, and phagolysosomes. C-CARS-EM imaging has much potential to study the interactions between a wide range of nanoparticles and cells with high precision and confidence.",
keywords = "FLUORESCENCE, IN-VITRO, LASER, LOCALIZATION, SPECTRA, SPECTROSCOPY, TOOL, cell imaging, cellular nanoparticle uptake, coherent anti-Stokes Raman scattering (CARS) microscopy, drug nanocrystals, non-linear imaging, 1182 Biochemistry, cell and molecular biology, 116 Chemical sciences, 317 Pharmacy",
author = "Saarinen, {Jukka Kalle Samuel} and Friederike G{\"u}tter and Lindman, {Mervi M} and Mikael Agopov and Fraser-Miller, {Sara J.} and Regina Scherlie{\ss} and Eija Jokitalo and {Almeida Santos}, Helder and Leena Peltonen and Antti Isom{\"a}ki and Strachan, {Clare J.}",
year = "2019",
month = "4",
doi = "10.1002/biot.201800413",
language = "English",
volume = "14",
journal = "Biotechnology Journal",
issn = "1860-6768",
publisher = "Wiley-VCH",
number = "4",

}

TY - JOUR

T1 - Cell-Nanoparticle Interactions at (Sub)-Nanometer Resolution Analyzed by Electron Microscopy and Correlative Coherent Anti-Stokes Raman Scattering

AU - Saarinen, Jukka Kalle Samuel

AU - Gütter, Friederike

AU - Lindman, Mervi M

AU - Agopov, Mikael

AU - Fraser-Miller, Sara J.

AU - Scherließ, Regina

AU - Jokitalo, Eija

AU - Almeida Santos, Helder

AU - Peltonen, Leena

AU - Isomäki, Antti

AU - Strachan, Clare J.

PY - 2019/4

Y1 - 2019/4

N2 - A wide variety of nanoparticles are playing an increasingly important role in drug delivery. Label-free imaging techniques are especially desirable to follow the cellular uptake and intracellular fate of nanoparticles. The combined correlative use of different techniques, each with unique advantages, facilitates more detailed investigation about such interactions. The synergistic use of correlative coherent anti-Stokes Raman scattering and electron microscopy (C-CARS-EM) imaging offers label-free, chemically-specific, and (sub)-nanometer spatial resolution for studying nanoparticle uptake into cells as demonstrated in the current study. Coherent anti-Stokes Raman scattering (CARS) microscopy offers chemically-specific (sub)micron spatial resolution imaging without fluorescent labels while transmission electron microscopy (TEM) offers (sub)-nanometer scale spatial resolution and thus visualization of precise nanoparticle localization at the sub-cellular level. This proof-of-concept imaging platform with unlabeled drug nanocrystals and macrophage cells revealed good colocalization between the CARS signal and electron dense nanocrystals in TEM images. The correlative TEM images revealed subcellular localization of nanocrystals inside membrane bound vesicles, showing multivesicular body (MVB)-like morphology typical for late endosomes (LEs), endolysosomes, and phagolysosomes. C-CARS-EM imaging has much potential to study the interactions between a wide range of nanoparticles and cells with high precision and confidence.

AB - A wide variety of nanoparticles are playing an increasingly important role in drug delivery. Label-free imaging techniques are especially desirable to follow the cellular uptake and intracellular fate of nanoparticles. The combined correlative use of different techniques, each with unique advantages, facilitates more detailed investigation about such interactions. The synergistic use of correlative coherent anti-Stokes Raman scattering and electron microscopy (C-CARS-EM) imaging offers label-free, chemically-specific, and (sub)-nanometer spatial resolution for studying nanoparticle uptake into cells as demonstrated in the current study. Coherent anti-Stokes Raman scattering (CARS) microscopy offers chemically-specific (sub)micron spatial resolution imaging without fluorescent labels while transmission electron microscopy (TEM) offers (sub)-nanometer scale spatial resolution and thus visualization of precise nanoparticle localization at the sub-cellular level. This proof-of-concept imaging platform with unlabeled drug nanocrystals and macrophage cells revealed good colocalization between the CARS signal and electron dense nanocrystals in TEM images. The correlative TEM images revealed subcellular localization of nanocrystals inside membrane bound vesicles, showing multivesicular body (MVB)-like morphology typical for late endosomes (LEs), endolysosomes, and phagolysosomes. C-CARS-EM imaging has much potential to study the interactions between a wide range of nanoparticles and cells with high precision and confidence.

KW - FLUORESCENCE

KW - IN-VITRO

KW - LASER

KW - LOCALIZATION

KW - SPECTRA

KW - SPECTROSCOPY

KW - TOOL

KW - cell imaging

KW - cellular nanoparticle uptake

KW - coherent anti-Stokes Raman scattering (CARS) microscopy

KW - drug nanocrystals

KW - non-linear imaging

KW - 1182 Biochemistry, cell and molecular biology

KW - 116 Chemical sciences

KW - 317 Pharmacy

U2 - 10.1002/biot.201800413

DO - 10.1002/biot.201800413

M3 - Article

VL - 14

JO - Biotechnology Journal

JF - Biotechnology Journal

SN - 1860-6768

IS - 4

M1 - 1800413

ER -