Kuvaus

Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part. Ten microliters of 50 mg/ml methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used. Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3´-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract
Alkuperäiskielienglanti
OtsikkoPlanta Medica
Vuosikerta81 - PM_77
KustantajaGeorg Thieme Verlag
Julkaisupäivä2015
DOI - pysyväislinkit
TilaJulkaistu - 2015
OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisuussa

Lisätietoja


Volume:
Proceeding volume: 81 - PM_77

Lainaa tätä

@inproceedings{55eab7f3dee54c42994368c911017114,
title = "Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF",
abstract = "Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part. Ten microliters of 50 mg/ml methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used. Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3´-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract",
author = "Salih, {Enass Yousif Abdelkarium} and Kanninen, {Markku Tapani} and Riitta Julkunen-Tiitto and Sipi, {Marketta Hannele} and Luukkanen, {Martti Olavi} and Vuorela, {Heikki Juhani} and Fyhrqvist, {Pia Johanna}",
note = "Volume: Proceeding volume: 81 - PM_77",
year = "2015",
doi = "10.1055/s-0035-1565454",
language = "English",
volume = "81 - PM_77",
booktitle = "Planta Medica",
publisher = "Georg Thieme Verlag",
address = "Germany",

}

Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF. / Salih, Enass Yousif Abdelkarium; Kanninen, Markku Tapani; Julkunen-Tiitto, Riitta; Sipi, Marketta Hannele; Luukkanen, Martti Olavi; Vuorela, Heikki Juhani; Fyhrqvist, Pia Johanna.

Planta Medica. Vuosikerta 81 - PM_77 Georg Thieme Verlag, 2015.

Tutkimustuotos: Artikkeli kirjassa/raportissa/konferenssijulkaisussaKonferenssiartikkeliTieteellinenvertaisarvioitu

TY - GEN

T1 - Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF

AU - Salih, Enass Yousif Abdelkarium

AU - Kanninen, Markku Tapani

AU - Julkunen-Tiitto, Riitta

AU - Sipi, Marketta Hannele

AU - Luukkanen, Martti Olavi

AU - Vuorela, Heikki Juhani

AU - Fyhrqvist, Pia Johanna

N1 - Volume: Proceeding volume: 81 - PM_77

PY - 2015

Y1 - 2015

N2 - Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part. Ten microliters of 50 mg/ml methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used. Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3´-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract

AB - Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part. Ten microliters of 50 mg/ml methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used. Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3´-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract

UR - https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0035-1565454

U2 - 10.1055/s-0035-1565454

DO - 10.1055/s-0035-1565454

M3 - Conference contribution

VL - 81 - PM_77

BT - Planta Medica

PB - Georg Thieme Verlag

ER -