Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice

Kati Hyvärinen, Anita Tuomainen, Saara Laitinen, Igor L Bykov, Liisa Tormakangas, Kai Lindros, Reijo Käkelä, Georg Alfthan, Irma Salminen, Matti Jauhiainen, Petri Kovanen, Maija Leinonen, Pekka Saikku, Pirkko Pussinen

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Periodontitis and Chlamydia pneumoniae infection are independent risk factors for cardiovascular diseases. The aim of this study was to investigate the effect of C. pneumoniae and Aggregatibacter actinomycetemcomitans infection on hepatic inflammation and lipid homeostasis of apolipoprotein E-deficient mice. Mice were infected with viable C. pneumoniae intranasally three times for chronic infection or once for acute infection. Viable A. actinomycetemcomitans was administered 10 times intravenously alone or in concert with C. pneumoniae. Hepatic alterations were assessed by histochemistry, lipid quantification, and fatty acid profile analysis. The RNA expression levels and the presence of pathogens in the livers and lungs were detected by quantitative real-time PCR. Both pathogens were detected in the livers of the infected animals. Chronic C. pneumoniae infection induced marked changes in hepatic lipid homeostasis. A. actinomycetemcomitans infection resulted in inflammatory cell infiltration into the liver, accompanied by elevated hepatic RNA expression levels of inflammation-related genes and higher serum amyloid A and lipopolysaccharide concentrations. Our results indicate that proatherogenic pathogens infect the liver, causing proinflammatory alterations and lipid disturbances. This infection may maintain chronic systemic inflammation attributable to atherogenesis.
Alkuperäiskielienglanti
LehtiInfection and Immunity
Vuosikerta77
Numero8
Sivut3442-3449
Sivumäärä8
ISSN0019-9567
DOI - pysyväislinkit
TilaJulkaistu - 2009
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 411 Maatalous ja metsätieteet
  • 118 Biotieteet

Lainaa tätä

Hyvärinen, Kati ; Tuomainen, Anita ; Laitinen, Saara ; Bykov, Igor L ; Tormakangas, Liisa ; Lindros, Kai ; Käkelä, Reijo ; Alfthan, Georg ; Salminen, Irma ; Jauhiainen, Matti ; Kovanen, Petri ; Leinonen, Maija ; Saikku, Pekka ; Pussinen, Pirkko. / Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice. Julkaisussa: Infection and Immunity. 2009 ; Vuosikerta 77, Nro 8. Sivut 3442-3449.
@article{6c10b4ec864342dd85c1d31b913cb256,
title = "Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice",
abstract = "Periodontitis and Chlamydia pneumoniae infection are independent risk factors for cardiovascular diseases. The aim of this study was to investigate the effect of C. pneumoniae and Aggregatibacter actinomycetemcomitans infection on hepatic inflammation and lipid homeostasis of apolipoprotein E-deficient mice. Mice were infected with viable C. pneumoniae intranasally three times for chronic infection or once for acute infection. Viable A. actinomycetemcomitans was administered 10 times intravenously alone or in concert with C. pneumoniae. Hepatic alterations were assessed by histochemistry, lipid quantification, and fatty acid profile analysis. The RNA expression levels and the presence of pathogens in the livers and lungs were detected by quantitative real-time PCR. Both pathogens were detected in the livers of the infected animals. Chronic C. pneumoniae infection induced marked changes in hepatic lipid homeostasis. A. actinomycetemcomitans infection resulted in inflammatory cell infiltration into the liver, accompanied by elevated hepatic RNA expression levels of inflammation-related genes and higher serum amyloid A and lipopolysaccharide concentrations. Our results indicate that proatherogenic pathogens infect the liver, causing proinflammatory alterations and lipid disturbances. This infection may maintain chronic systemic inflammation attributable to atherogenesis.",
keywords = "411 Agriculture and forestry, 118 Biological sciences",
author = "Kati Hyv{\"a}rinen and Anita Tuomainen and Saara Laitinen and Bykov, {Igor L} and Liisa Tormakangas and Kai Lindros and Reijo K{\"a}kel{\"a} and Georg Alfthan and Irma Salminen and Matti Jauhiainen and Petri Kovanen and Maija Leinonen and Pekka Saikku and Pirkko Pussinen",
year = "2009",
doi = "10.1128/IAI.00389-09",
language = "English",
volume = "77",
pages = "3442--3449",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "8",

}

Hyvärinen, K, Tuomainen, A, Laitinen, S, Bykov, IL, Tormakangas, L, Lindros, K, Käkelä, R, Alfthan, G, Salminen, I, Jauhiainen, M, Kovanen, P, Leinonen, M, Saikku, P & Pussinen, P 2009, 'Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice', Infection and Immunity, Vuosikerta 77, Nro 8, Sivut 3442-3449. https://doi.org/10.1128/IAI.00389-09

Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice. / Hyvärinen, Kati; Tuomainen, Anita; Laitinen, Saara; Bykov, Igor L; Tormakangas, Liisa; Lindros, Kai; Käkelä, Reijo; Alfthan, Georg; Salminen, Irma; Jauhiainen, Matti; Kovanen, Petri; Leinonen, Maija; Saikku, Pekka; Pussinen, Pirkko.

julkaisussa: Infection and Immunity, Vuosikerta 77, Nro 8, 2009, s. 3442-3449.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice

AU - Hyvärinen, Kati

AU - Tuomainen, Anita

AU - Laitinen, Saara

AU - Bykov, Igor L

AU - Tormakangas, Liisa

AU - Lindros, Kai

AU - Käkelä, Reijo

AU - Alfthan, Georg

AU - Salminen, Irma

AU - Jauhiainen, Matti

AU - Kovanen, Petri

AU - Leinonen, Maija

AU - Saikku, Pekka

AU - Pussinen, Pirkko

PY - 2009

Y1 - 2009

N2 - Periodontitis and Chlamydia pneumoniae infection are independent risk factors for cardiovascular diseases. The aim of this study was to investigate the effect of C. pneumoniae and Aggregatibacter actinomycetemcomitans infection on hepatic inflammation and lipid homeostasis of apolipoprotein E-deficient mice. Mice were infected with viable C. pneumoniae intranasally three times for chronic infection or once for acute infection. Viable A. actinomycetemcomitans was administered 10 times intravenously alone or in concert with C. pneumoniae. Hepatic alterations were assessed by histochemistry, lipid quantification, and fatty acid profile analysis. The RNA expression levels and the presence of pathogens in the livers and lungs were detected by quantitative real-time PCR. Both pathogens were detected in the livers of the infected animals. Chronic C. pneumoniae infection induced marked changes in hepatic lipid homeostasis. A. actinomycetemcomitans infection resulted in inflammatory cell infiltration into the liver, accompanied by elevated hepatic RNA expression levels of inflammation-related genes and higher serum amyloid A and lipopolysaccharide concentrations. Our results indicate that proatherogenic pathogens infect the liver, causing proinflammatory alterations and lipid disturbances. This infection may maintain chronic systemic inflammation attributable to atherogenesis.

AB - Periodontitis and Chlamydia pneumoniae infection are independent risk factors for cardiovascular diseases. The aim of this study was to investigate the effect of C. pneumoniae and Aggregatibacter actinomycetemcomitans infection on hepatic inflammation and lipid homeostasis of apolipoprotein E-deficient mice. Mice were infected with viable C. pneumoniae intranasally three times for chronic infection or once for acute infection. Viable A. actinomycetemcomitans was administered 10 times intravenously alone or in concert with C. pneumoniae. Hepatic alterations were assessed by histochemistry, lipid quantification, and fatty acid profile analysis. The RNA expression levels and the presence of pathogens in the livers and lungs were detected by quantitative real-time PCR. Both pathogens were detected in the livers of the infected animals. Chronic C. pneumoniae infection induced marked changes in hepatic lipid homeostasis. A. actinomycetemcomitans infection resulted in inflammatory cell infiltration into the liver, accompanied by elevated hepatic RNA expression levels of inflammation-related genes and higher serum amyloid A and lipopolysaccharide concentrations. Our results indicate that proatherogenic pathogens infect the liver, causing proinflammatory alterations and lipid disturbances. This infection may maintain chronic systemic inflammation attributable to atherogenesis.

KW - 411 Agriculture and forestry

KW - 118 Biological sciences

U2 - 10.1128/IAI.00389-09

DO - 10.1128/IAI.00389-09

M3 - Article

VL - 77

SP - 3442

EP - 3449

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 8

ER -