Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins

Timo Antero Lehti, Benita Westerlund-Wikström

Tutkimustuotos: ArtikkelijulkaisuArtikkeliAmmatillinen

Abstrakti

This simple protocol describes how to detect antigens from agar-grown bacterial colonies transferred to nitrocellulose using specific antibodies. The protocol is well suitable for detection of bacterial proteins exposed on the cell surface or secreted to the extracellular space and it can be modified also for detection of intracellular proteins. The assay can distinguish bacterial clones with different expression rates (high, medium and low) from colonies that do not express target protein. We used this assay for screening of Mat fimbriae-producing Escherichia coli mutants obtained by mini-Tn5 transposon mutagenesis and immunomagnetic separation (Lehti et al., 2013).
Alkuperäiskielienglanti
LehtiBio-Protocol
Vuosikerta3
Numero17
TilaJulkaistu - 5 syysk. 2013
OKM-julkaisutyyppiD1 Artikkeli ammattilehdessä

Tieteenalat

  • 1183 Kasvibiologia, mikrobiologia, virologia

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