Kuvaus

Non-aureus staphylococci (NAS) are most commonly isolated from subclinical mastitis. Different NAS species may, however, have diverse effects on the inflammatory response in the udder. We determined the genome sequences of 20 staphylococcal isolates from clinical or subclinical bovine mastitis, belonging to the NAS species Staphylococcus agnetis S. chromogenes, and S. simulans, and focused on the putative virulence factor genes present in the genomes. For comparison we used our previously published genome sequences of four S. aureus isolates from bovine mastitis. The pan-genome and core genomes of the non-aureus isolates were characterized. After that, putative virulence factor orthologues were searched in silico. We compared the presence of putative virulence factors in the NAS species and S. aureus and evaluated the potential association between bacterial genotype and type of mastitis (clinical vs. subclinical). The NAS isolates had much less virulence gene orthologues than the S. aureus isolates. One third of the virulence genes were detected only in S. aureus. About 100 virulence genes were present in all S. aureus isolates, compared to about 40 to 50 in each NAS isolate. S. simulans differed the most. Several of the virulence genes detected among NAS were harbored only by S. simulans, but it also lacked a number of genes present both in S. agnetis and S. chromogenes. The type of mastitis was not associated with any specific virulence gene profile. It seems that the virulence gene profiles or cumulative number of different virulence genes are not directly associated with the type of mastitis (clinical or)subclinical), indicating that host derived factors such as the immune status play a pivotal role in the manifestation of mastitis.
Alkuperäiskielienglanti
Artikkelie4560
LehtiPeerJ
Vuosikerta6
Sivumäärä31
ISSN2167-8359
DOI - pysyväislinkit
TilaJulkaistu - 30 maaliskuuta 2018
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

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@article{1e53c9c8629c4801b6ae7c3cf47632a1,
title = "Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes",
abstract = "Non-aureus staphylococci (NAS) are most commonly isolated from subclinical mastitis. Different NAS species may, however, have diverse effects on the inflammatory response in the udder. We determined the genome sequences of 20 staphylococcal isolates from clinical or subclinical bovine mastitis, belonging to the NAS species Staphylococcus agnetis S. chromogenes, and S. simulans, and focused on the putative virulence factor genes present in the genomes. For comparison we used our previously published genome sequences of four S. aureus isolates from bovine mastitis. The pan-genome and core genomes of the non-aureus isolates were characterized. After that, putative virulence factor orthologues were searched in silico. We compared the presence of putative virulence factors in the NAS species and S. aureus and evaluated the potential association between bacterial genotype and type of mastitis (clinical vs. subclinical). The NAS isolates had much less virulence gene orthologues than the S. aureus isolates. One third of the virulence genes were detected only in S. aureus. About 100 virulence genes were present in all S. aureus isolates, compared to about 40 to 50 in each NAS isolate. S. simulans differed the most. Several of the virulence genes detected among NAS were harbored only by S. simulans, but it also lacked a number of genes present both in S. agnetis and S. chromogenes. The type of mastitis was not associated with any specific virulence gene profile. It seems that the virulence gene profiles or cumulative number of different virulence genes are not directly associated with the type of mastitis (clinical or)subclinical), indicating that host derived factors such as the immune status play a pivotal role in the manifestation of mastitis.",
keywords = "413 Veterinary science, Staphylococcus, Virulence factor, Comparison, non-aureus staphylococci, COAGULASE-NEGATIVE STAPHYLOCOCCI, FIELD GEL-ELECTROPHORESIS, SOMATIC-CELL COUNT, INTRAMAMMARY INFECTION, SUBCLINICAL MASTITIS, AUREUS STRAINS, DAIRY HEIFERS, ANTIMICROBIAL SUSCEPTIBILITY, CLINICAL CHARACTERISTICS, ANTIBIOTIC-RESISTANCE",
author = "{\AA}vall-J{\"a}{\"a}skel{\"a}inen, {Silja Tuulia} and Taponen, {Suvi Sinikka} and Ravi Kant and Paulin, {Lars G{\"o}ran} and Jochen Blom and Palva, {Airi Marjatta} and Koort, {Joanna Maria Kataliina}",
year = "2018",
month = "3",
day = "30",
doi = "10.7717/peerj.4560",
language = "English",
volume = "6",
journal = "PeerJ",
issn = "2167-8359",
publisher = "PeerJ Inc.",

}

TY - JOUR

T1 - Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes

AU - Åvall-Jääskeläinen, Silja Tuulia

AU - Taponen, Suvi Sinikka

AU - Kant, Ravi

AU - Paulin, Lars Göran

AU - Blom, Jochen

AU - Palva, Airi Marjatta

AU - Koort, Joanna Maria Kataliina

PY - 2018/3/30

Y1 - 2018/3/30

N2 - Non-aureus staphylococci (NAS) are most commonly isolated from subclinical mastitis. Different NAS species may, however, have diverse effects on the inflammatory response in the udder. We determined the genome sequences of 20 staphylococcal isolates from clinical or subclinical bovine mastitis, belonging to the NAS species Staphylococcus agnetis S. chromogenes, and S. simulans, and focused on the putative virulence factor genes present in the genomes. For comparison we used our previously published genome sequences of four S. aureus isolates from bovine mastitis. The pan-genome and core genomes of the non-aureus isolates were characterized. After that, putative virulence factor orthologues were searched in silico. We compared the presence of putative virulence factors in the NAS species and S. aureus and evaluated the potential association between bacterial genotype and type of mastitis (clinical vs. subclinical). The NAS isolates had much less virulence gene orthologues than the S. aureus isolates. One third of the virulence genes were detected only in S. aureus. About 100 virulence genes were present in all S. aureus isolates, compared to about 40 to 50 in each NAS isolate. S. simulans differed the most. Several of the virulence genes detected among NAS were harbored only by S. simulans, but it also lacked a number of genes present both in S. agnetis and S. chromogenes. The type of mastitis was not associated with any specific virulence gene profile. It seems that the virulence gene profiles or cumulative number of different virulence genes are not directly associated with the type of mastitis (clinical or)subclinical), indicating that host derived factors such as the immune status play a pivotal role in the manifestation of mastitis.

AB - Non-aureus staphylococci (NAS) are most commonly isolated from subclinical mastitis. Different NAS species may, however, have diverse effects on the inflammatory response in the udder. We determined the genome sequences of 20 staphylococcal isolates from clinical or subclinical bovine mastitis, belonging to the NAS species Staphylococcus agnetis S. chromogenes, and S. simulans, and focused on the putative virulence factor genes present in the genomes. For comparison we used our previously published genome sequences of four S. aureus isolates from bovine mastitis. The pan-genome and core genomes of the non-aureus isolates were characterized. After that, putative virulence factor orthologues were searched in silico. We compared the presence of putative virulence factors in the NAS species and S. aureus and evaluated the potential association between bacterial genotype and type of mastitis (clinical vs. subclinical). The NAS isolates had much less virulence gene orthologues than the S. aureus isolates. One third of the virulence genes were detected only in S. aureus. About 100 virulence genes were present in all S. aureus isolates, compared to about 40 to 50 in each NAS isolate. S. simulans differed the most. Several of the virulence genes detected among NAS were harbored only by S. simulans, but it also lacked a number of genes present both in S. agnetis and S. chromogenes. The type of mastitis was not associated with any specific virulence gene profile. It seems that the virulence gene profiles or cumulative number of different virulence genes are not directly associated with the type of mastitis (clinical or)subclinical), indicating that host derived factors such as the immune status play a pivotal role in the manifestation of mastitis.

KW - 413 Veterinary science

KW - Staphylococcus

KW - Virulence factor

KW - Comparison

KW - non-aureus staphylococci

KW - COAGULASE-NEGATIVE STAPHYLOCOCCI

KW - FIELD GEL-ELECTROPHORESIS

KW - SOMATIC-CELL COUNT

KW - INTRAMAMMARY INFECTION

KW - SUBCLINICAL MASTITIS

KW - AUREUS STRAINS

KW - DAIRY HEIFERS

KW - ANTIMICROBIAL SUSCEPTIBILITY

KW - CLINICAL CHARACTERISTICS

KW - ANTIBIOTIC-RESISTANCE

U2 - 10.7717/peerj.4560

DO - 10.7717/peerj.4560

M3 - Article

VL - 6

JO - PeerJ

JF - PeerJ

SN - 2167-8359

M1 - e4560

ER -