Development of chloroplast microsatellite markers for giant ragweed (Ambrosia trifida)

Himanshu Sharma, Jaakko Hyvönen, Péter Poczai

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Premise Plant invasions are increasing globally, and extensive study of the genetic background of the source and invading populations is needed to understand such biological processes. For this reason, chloroplast microsatellite markers were identified to explore the genetic diversity of the noxious weed Ambrosia trifida (Asteraceae). Methods and Results The complete chloroplast genome of A. trifida was mined for microsatellite loci, and 15 novel chloroplast primers were identified to assess the genetic diversity of 49 Ambrosia samples. The number of alleles amplified ranged from two to six, with an average of 3.2 alleles per locus. Shannon's information index varied from 0.305 and 1.467, expected heterozygosity ranged from 0.178 to 0.645, and the polymorphism information content value ranged from 0.211 to 0.675 (average 0.428). The cross-species transferability of the 15 microsatellite loci was also evaluated in four related Ambrosia species (A. artemisiifolia, A. maritima, A. psilostachya, and A. tenuifolia). Conclusions The novel chloroplast microsatellite markers developed in the current study demonstrate substantial cross-species transferability and will be helpful in future genetic diversity studies of A. trifida and related species.

Alkuperäiskielienglanti
Artikkeli11313
LehtiApplications in Plant Sciences
Vuosikerta8
Numero1
Sivumäärä4
ISSN2168-0450
DOI - pysyväislinkit
TilaJulkaistu - tammikuuta 2020
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1181 Ekologia, evoluutiobiologia
  • 414 Maatalouden bioteknologia

Lainaa tätä

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title = "Development of chloroplast microsatellite markers for giant ragweed (Ambrosia trifida)",
abstract = "Premise Plant invasions are increasing globally, and extensive study of the genetic background of the source and invading populations is needed to understand such biological processes. For this reason, chloroplast microsatellite markers were identified to explore the genetic diversity of the noxious weed Ambrosia trifida (Asteraceae). Methods and Results The complete chloroplast genome of A. trifida was mined for microsatellite loci, and 15 novel chloroplast primers were identified to assess the genetic diversity of 49 Ambrosia samples. The number of alleles amplified ranged from two to six, with an average of 3.2 alleles per locus. Shannon's information index varied from 0.305 and 1.467, expected heterozygosity ranged from 0.178 to 0.645, and the polymorphism information content value ranged from 0.211 to 0.675 (average 0.428). The cross-species transferability of the 15 microsatellite loci was also evaluated in four related Ambrosia species (A. artemisiifolia, A. maritima, A. psilostachya, and A. tenuifolia). Conclusions The novel chloroplast microsatellite markers developed in the current study demonstrate substantial cross-species transferability and will be helpful in future genetic diversity studies of A. trifida and related species.",
keywords = "ARTEMISIIFOLIA, Ambrosia trifida, Asteraceae, COMMON RAGWEED, GENOME, microsatellites, molecular markers, noxious weeds, plant invasions, plastid, polymorphism, 1181 Ecology, evolutionary biology, 414 Agricultural biotechnology",
author = "Himanshu Sharma and Jaakko Hyv{\"o}nen and P{\'e}ter Poczai",
year = "2020",
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doi = "10.1002/aps3.11313",
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volume = "8",
journal = "Applications in Plant Sciences",
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Development of chloroplast microsatellite markers for giant ragweed (Ambrosia trifida). / Sharma, Himanshu; Hyvönen, Jaakko; Poczai, Péter.

julkaisussa: Applications in Plant Sciences, Vuosikerta 8, Nro 1, 11313, 01.2020.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Development of chloroplast microsatellite markers for giant ragweed (Ambrosia trifida)

AU - Sharma, Himanshu

AU - Hyvönen, Jaakko

AU - Poczai, Péter

PY - 2020/1

Y1 - 2020/1

N2 - Premise Plant invasions are increasing globally, and extensive study of the genetic background of the source and invading populations is needed to understand such biological processes. For this reason, chloroplast microsatellite markers were identified to explore the genetic diversity of the noxious weed Ambrosia trifida (Asteraceae). Methods and Results The complete chloroplast genome of A. trifida was mined for microsatellite loci, and 15 novel chloroplast primers were identified to assess the genetic diversity of 49 Ambrosia samples. The number of alleles amplified ranged from two to six, with an average of 3.2 alleles per locus. Shannon's information index varied from 0.305 and 1.467, expected heterozygosity ranged from 0.178 to 0.645, and the polymorphism information content value ranged from 0.211 to 0.675 (average 0.428). The cross-species transferability of the 15 microsatellite loci was also evaluated in four related Ambrosia species (A. artemisiifolia, A. maritima, A. psilostachya, and A. tenuifolia). Conclusions The novel chloroplast microsatellite markers developed in the current study demonstrate substantial cross-species transferability and will be helpful in future genetic diversity studies of A. trifida and related species.

AB - Premise Plant invasions are increasing globally, and extensive study of the genetic background of the source and invading populations is needed to understand such biological processes. For this reason, chloroplast microsatellite markers were identified to explore the genetic diversity of the noxious weed Ambrosia trifida (Asteraceae). Methods and Results The complete chloroplast genome of A. trifida was mined for microsatellite loci, and 15 novel chloroplast primers were identified to assess the genetic diversity of 49 Ambrosia samples. The number of alleles amplified ranged from two to six, with an average of 3.2 alleles per locus. Shannon's information index varied from 0.305 and 1.467, expected heterozygosity ranged from 0.178 to 0.645, and the polymorphism information content value ranged from 0.211 to 0.675 (average 0.428). The cross-species transferability of the 15 microsatellite loci was also evaluated in four related Ambrosia species (A. artemisiifolia, A. maritima, A. psilostachya, and A. tenuifolia). Conclusions The novel chloroplast microsatellite markers developed in the current study demonstrate substantial cross-species transferability and will be helpful in future genetic diversity studies of A. trifida and related species.

KW - ARTEMISIIFOLIA

KW - Ambrosia trifida

KW - Asteraceae

KW - COMMON RAGWEED

KW - GENOME

KW - microsatellites

KW - molecular markers

KW - noxious weeds

KW - plant invasions

KW - plastid

KW - polymorphism

KW - 1181 Ecology, evolutionary biology

KW - 414 Agricultural biotechnology

U2 - 10.1002/aps3.11313

DO - 10.1002/aps3.11313

M3 - Article

VL - 8

JO - Applications in Plant Sciences

JF - Applications in Plant Sciences

SN - 2168-0450

IS - 1

M1 - 11313

ER -