Electron dispersive X-ray spectroscopy and degradation properties of hyaluronic acid decorated microparticles

Carla Serri, Mariaenrica Frigione, Marika Ruponen, Arto Urtti, Assunta Borzacchiello, Marco Biondi, Jaakko Itkonen, Laura Mayol

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

The purpose of this study was to produce poly(DL-lactic-co-glycolic acid) (PLGA) – based microparticles (MPs), externally decorated with hyaluronic acid (HA). The MPs are intended for intravitreal injections in the treatment of posterior eye segment and have been designed to prolong the release of growth factors into the vitreous body, therefore aiming to increase the time interval between two consecutive injections. The MPs, prepared by a modified double emulsion-solvent evaporation technique and loaded with bovine serum albumins (BSA) and ciliary neurotrophic factor (CNTF), were spherical, with a diameter around 70 μm and a > 90% encapsulation efficiency. Energy Dispersive Spectroscopy (EDS) outcomes indicated that HA presence in the external aqueous phase of the emulsion did affect the surface properties of MPs. Moreover, poloxamers drastically slowed down MP degradation properties which are, in turn, closely related to their ability to prolong drug release. This is promising for the envisaged application of the produced MPs. Further work will be devoted to optimizing MP formulation with respect to the envisaged intravitreal route of administration.
Alkuperäiskielienglanti
LehtiColloids and Surfaces B: Biointerfaces
Vuosikerta181
Sivut896-901
Sivumäärä6
ISSN0927-7765
DOI - pysyväislinkit
TilaJulkaistu - 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • hyaluronic acid
  • microparticle
  • PLGA
  • poloxamer
  • diffusion
  • ocular delivery
  • 1182 Biokemia, solu- ja molekyylibiologia
  • 317 Farmasia

Lainaa tätä

Serri, Carla ; Frigione, Mariaenrica ; Ruponen, Marika ; Urtti, Arto ; Borzacchiello, Assunta ; Biondi, Marco ; Itkonen, Jaakko ; Mayol, Laura. / Electron dispersive X-ray spectroscopy and degradation properties of hyaluronic acid decorated microparticles. Julkaisussa: Colloids and Surfaces B: Biointerfaces. 2019 ; Vuosikerta 181. Sivut 896-901.
@article{82baa3439eae40848fcd4658d537fc28,
title = "Electron dispersive X-ray spectroscopy and degradation properties of hyaluronic acid decorated microparticles",
abstract = "The purpose of this study was to produce poly(DL-lactic-co-glycolic acid) (PLGA) – based microparticles (MPs), externally decorated with hyaluronic acid (HA). The MPs are intended for intravitreal injections in the treatment of posterior eye segment and have been designed to prolong the release of growth factors into the vitreous body, therefore aiming to increase the time interval between two consecutive injections. The MPs, prepared by a modified double emulsion-solvent evaporation technique and loaded with bovine serum albumins (BSA) and ciliary neurotrophic factor (CNTF), were spherical, with a diameter around 70 μm and a > 90{\%} encapsulation efficiency. Energy Dispersive Spectroscopy (EDS) outcomes indicated that HA presence in the external aqueous phase of the emulsion did affect the surface properties of MPs. Moreover, poloxamers drastically slowed down MP degradation properties which are, in turn, closely related to their ability to prolong drug release. This is promising for the envisaged application of the produced MPs. Further work will be devoted to optimizing MP formulation with respect to the envisaged intravitreal route of administration.",
keywords = "hyaluronic acid, microparticle, PLGA, poloxamer, diffusion, ocular delivery, Hyaluronic acid, Microparticle, PLGA, Poloxamer, Diffusion, Ocular delivery, 1182 Biochemistry, cell and molecular biology, 317 Pharmacy",
author = "Carla Serri and Mariaenrica Frigione and Marika Ruponen and Arto Urtti and Assunta Borzacchiello and Marco Biondi and Jaakko Itkonen and Laura Mayol",
year = "2019",
doi = "10.1016/j.colsurfb.2019.06.044",
language = "English",
volume = "181",
pages = "896--901",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier Scientific Publ. Co",

}

Electron dispersive X-ray spectroscopy and degradation properties of hyaluronic acid decorated microparticles. / Serri, Carla; Frigione, Mariaenrica; Ruponen, Marika; Urtti, Arto; Borzacchiello, Assunta; Biondi, Marco; Itkonen, Jaakko; Mayol, Laura.

julkaisussa: Colloids and Surfaces B: Biointerfaces, Vuosikerta 181, 2019, s. 896-901.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Electron dispersive X-ray spectroscopy and degradation properties of hyaluronic acid decorated microparticles

AU - Serri, Carla

AU - Frigione, Mariaenrica

AU - Ruponen, Marika

AU - Urtti, Arto

AU - Borzacchiello, Assunta

AU - Biondi, Marco

AU - Itkonen, Jaakko

AU - Mayol, Laura

PY - 2019

Y1 - 2019

N2 - The purpose of this study was to produce poly(DL-lactic-co-glycolic acid) (PLGA) – based microparticles (MPs), externally decorated with hyaluronic acid (HA). The MPs are intended for intravitreal injections in the treatment of posterior eye segment and have been designed to prolong the release of growth factors into the vitreous body, therefore aiming to increase the time interval between two consecutive injections. The MPs, prepared by a modified double emulsion-solvent evaporation technique and loaded with bovine serum albumins (BSA) and ciliary neurotrophic factor (CNTF), were spherical, with a diameter around 70 μm and a > 90% encapsulation efficiency. Energy Dispersive Spectroscopy (EDS) outcomes indicated that HA presence in the external aqueous phase of the emulsion did affect the surface properties of MPs. Moreover, poloxamers drastically slowed down MP degradation properties which are, in turn, closely related to their ability to prolong drug release. This is promising for the envisaged application of the produced MPs. Further work will be devoted to optimizing MP formulation with respect to the envisaged intravitreal route of administration.

AB - The purpose of this study was to produce poly(DL-lactic-co-glycolic acid) (PLGA) – based microparticles (MPs), externally decorated with hyaluronic acid (HA). The MPs are intended for intravitreal injections in the treatment of posterior eye segment and have been designed to prolong the release of growth factors into the vitreous body, therefore aiming to increase the time interval between two consecutive injections. The MPs, prepared by a modified double emulsion-solvent evaporation technique and loaded with bovine serum albumins (BSA) and ciliary neurotrophic factor (CNTF), were spherical, with a diameter around 70 μm and a > 90% encapsulation efficiency. Energy Dispersive Spectroscopy (EDS) outcomes indicated that HA presence in the external aqueous phase of the emulsion did affect the surface properties of MPs. Moreover, poloxamers drastically slowed down MP degradation properties which are, in turn, closely related to their ability to prolong drug release. This is promising for the envisaged application of the produced MPs. Further work will be devoted to optimizing MP formulation with respect to the envisaged intravitreal route of administration.

KW - hyaluronic acid

KW - microparticle

KW - PLGA

KW - poloxamer

KW - diffusion

KW - ocular delivery

KW - Hyaluronic acid

KW - Microparticle

KW - PLGA

KW - Poloxamer

KW - Diffusion

KW - Ocular delivery

KW - 1182 Biochemistry, cell and molecular biology

KW - 317 Pharmacy

U2 - 10.1016/j.colsurfb.2019.06.044

DO - 10.1016/j.colsurfb.2019.06.044

M3 - Article

VL - 181

SP - 896

EP - 901

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -