Ellagitannins in antimycobacterial extracts of Combretum hartmannianum, a savannah woodland tree

Tutkimustuotos: KonferenssimateriaalitPosteriTutkimusvertaisarvioitu

Kuvaus

Combretum hartmannianum Schweinf. (Combretaceae) is native to East Africa, growing naturally in woodland areas in Eritrea, Ethiopia, South Sudan and Sudan [1]. Various parts of this plant, including roots and stem are used traditionally in Africa against bacterial infections and their symptoms such as cough [2].
We have assessed the antimycobacterial activity of extracts of C. hartmannium against the TB model bacterium Mycobacterium smegmatis ATCC 14468 . The most promising growth inhibitory activity was observed for root extracts, such as a methanol soxhlet extract (IZ 31.5mm, MIC 312.5 µg/ml) and an ethyl acetate extract (MIC 1250 µg/ml). Cold methanol extracts of the stem bark and wood gave a MIC of 5000 µg/ml. Hexane and dichloromethane extracts of the stem wood and stem bark were devoid of activity. HPLC-DAD and UHPLC/QTOF-MS analysis of the ethyl acetate extract of the root resulted in the characterization of fifteen ellagitannins, among them punicalagin, castalagin, corilagin, tellimagrandin, terflavin B and S-flavogallonic acid, none of which have been identified before in C. hartmannianum. Six of the identified ellagitannins are unknown and NMR is needed to elucidate their molecular structures. Previously, flavogallonic acid dilactone and terchebulin have been identified in C. hartmannianum stem bark [3]. Our results indicate that roots of C. hartmannianum contain a high diversity and concentration of ellagitannins which could be potential agents against tuberculosis.
Alkuperäiskielienglanti
Sivumäärä1
TilaJulkaistu - 27 elokuuta 2018
OKM-julkaisutyyppiEi sovellu
Tapahtuma66th Annual Meeting of the Society for Medicinal Plant and Natural product research - Shanghai, China, Shanghai, Kiina
Kesto: 26 elokuuta 201830 elokuuta 2018
Konferenssinumero: 66
http://www.ga2018.cn/weben/

Konferenssi

Konferenssi66th Annual Meeting of the Society for Medicinal Plant and Natural product research
LyhennettäGA 2018
MaaKiina
KaupunkiShanghai
Ajanjakso26/08/201830/08/2018
www-osoite

Tieteenalat

  • 4112 Metsätiede
  • 317 Farmasia

Lainaa tätä

Salih, E. Y. A., Kanninen, M. T., Julkunen-Tiitto, R., Sipi, M. H., Luukkanen, M. O., Vuorela, H. J., & Fyhrqvist, P. J. (2018). Ellagitannins in antimycobacterial extracts of Combretum hartmannianum, a savannah woodland tree. Posterin esittämispaikka: 66th Annual Meeting of the Society for Medicinal Plant and Natural product research, Shanghai, Kiina.
Salih, Enass Yousif Abdelkarium ; Kanninen, Markku Tapani ; Julkunen-Tiitto, Riitta ; Sipi, Marketta Hannele ; Luukkanen, Martti Olavi ; Vuorela, Heikki Juhani ; Fyhrqvist, Pia Johanna. / Ellagitannins in antimycobacterial extracts of Combretum hartmannianum, a savannah woodland tree. Posterin esittämispaikka: 66th Annual Meeting of the Society for Medicinal Plant and Natural product research, Shanghai, Kiina.1 Sivumäärä
@conference{d45a994ac8fd4c16aaf9e232e3dd276c,
title = "Ellagitannins in antimycobacterial extracts of Combretum hartmannianum, a savannah woodland tree",
abstract = "Combretum hartmannianum Schweinf. (Combretaceae) is native to East Africa, growing naturally in woodland areas in Eritrea, Ethiopia, South Sudan and Sudan [1]. Various parts of this plant, including roots and stem are used traditionally in Africa against bacterial infections and their symptoms such as cough [2].We have assessed the antimycobacterial activity of extracts of C. hartmannium against the TB model bacterium Mycobacterium smegmatis ATCC 14468 . The most promising growth inhibitory activity was observed for root extracts, such as a methanol soxhlet extract (IZ 31.5mm, MIC 312.5 µg/ml) and an ethyl acetate extract (MIC 1250 µg/ml). Cold methanol extracts of the stem bark and wood gave a MIC of 5000 µg/ml. Hexane and dichloromethane extracts of the stem wood and stem bark were devoid of activity. HPLC-DAD and UHPLC/QTOF-MS analysis of the ethyl acetate extract of the root resulted in the characterization of fifteen ellagitannins, among them punicalagin, castalagin, corilagin, tellimagrandin, terflavin B and S-flavogallonic acid, none of which have been identified before in C. hartmannianum. Six of the identified ellagitannins are unknown and NMR is needed to elucidate their molecular structures. Previously, flavogallonic acid dilactone and terchebulin have been identified in C. hartmannianum stem bark [3]. Our results indicate that roots of C. hartmannianum contain a high diversity and concentration of ellagitannins which could be potential agents against tuberculosis.",
keywords = "4112 Forestry, 317 Pharmacy",
author = "Salih, {Enass Yousif Abdelkarium} and Kanninen, {Markku Tapani} and Riitta Julkunen-Tiitto and Sipi, {Marketta Hannele} and Luukkanen, {Martti Olavi} and Vuorela, {Heikki Juhani} and Fyhrqvist, {Pia Johanna}",
year = "2018",
month = "8",
day = "27",
language = "English",
note = "66th Annual Meeting of the Society for Medicinal Plant and Natural product research, GA 2018 ; Conference date: 26-08-2018 Through 30-08-2018",
url = "http://www.ga2018.cn/weben/",

}

Salih, EYA, Kanninen, MT, Julkunen-Tiitto, R, Sipi, MH, Luukkanen, MO, Vuorela, HJ & Fyhrqvist, PJ 2018, 'Ellagitannins in antimycobacterial extracts of Combretum hartmannianum, a savannah woodland tree' 66th Annual Meeting of the Society for Medicinal Plant and Natural product research, Shanghai, Kiina, 26/08/2018 - 30/08/2018, .

Ellagitannins in antimycobacterial extracts of Combretum hartmannianum, a savannah woodland tree. / Salih, Enass Yousif Abdelkarium; Kanninen, Markku Tapani; Julkunen-Tiitto, Riitta; Sipi, Marketta Hannele; Luukkanen, Martti Olavi; Vuorela, Heikki Juhani; Fyhrqvist, Pia Johanna.

2018. Posterin esittämispaikka: 66th Annual Meeting of the Society for Medicinal Plant and Natural product research, Shanghai, Kiina.

Tutkimustuotos: KonferenssimateriaalitPosteriTutkimusvertaisarvioitu

TY - CONF

T1 - Ellagitannins in antimycobacterial extracts of Combretum hartmannianum, a savannah woodland tree

AU - Salih, Enass Yousif Abdelkarium

AU - Kanninen, Markku Tapani

AU - Julkunen-Tiitto, Riitta

AU - Sipi, Marketta Hannele

AU - Luukkanen, Martti Olavi

AU - Vuorela, Heikki Juhani

AU - Fyhrqvist, Pia Johanna

PY - 2018/8/27

Y1 - 2018/8/27

N2 - Combretum hartmannianum Schweinf. (Combretaceae) is native to East Africa, growing naturally in woodland areas in Eritrea, Ethiopia, South Sudan and Sudan [1]. Various parts of this plant, including roots and stem are used traditionally in Africa against bacterial infections and their symptoms such as cough [2].We have assessed the antimycobacterial activity of extracts of C. hartmannium against the TB model bacterium Mycobacterium smegmatis ATCC 14468 . The most promising growth inhibitory activity was observed for root extracts, such as a methanol soxhlet extract (IZ 31.5mm, MIC 312.5 µg/ml) and an ethyl acetate extract (MIC 1250 µg/ml). Cold methanol extracts of the stem bark and wood gave a MIC of 5000 µg/ml. Hexane and dichloromethane extracts of the stem wood and stem bark were devoid of activity. HPLC-DAD and UHPLC/QTOF-MS analysis of the ethyl acetate extract of the root resulted in the characterization of fifteen ellagitannins, among them punicalagin, castalagin, corilagin, tellimagrandin, terflavin B and S-flavogallonic acid, none of which have been identified before in C. hartmannianum. Six of the identified ellagitannins are unknown and NMR is needed to elucidate their molecular structures. Previously, flavogallonic acid dilactone and terchebulin have been identified in C. hartmannianum stem bark [3]. Our results indicate that roots of C. hartmannianum contain a high diversity and concentration of ellagitannins which could be potential agents against tuberculosis.

AB - Combretum hartmannianum Schweinf. (Combretaceae) is native to East Africa, growing naturally in woodland areas in Eritrea, Ethiopia, South Sudan and Sudan [1]. Various parts of this plant, including roots and stem are used traditionally in Africa against bacterial infections and their symptoms such as cough [2].We have assessed the antimycobacterial activity of extracts of C. hartmannium against the TB model bacterium Mycobacterium smegmatis ATCC 14468 . The most promising growth inhibitory activity was observed for root extracts, such as a methanol soxhlet extract (IZ 31.5mm, MIC 312.5 µg/ml) and an ethyl acetate extract (MIC 1250 µg/ml). Cold methanol extracts of the stem bark and wood gave a MIC of 5000 µg/ml. Hexane and dichloromethane extracts of the stem wood and stem bark were devoid of activity. HPLC-DAD and UHPLC/QTOF-MS analysis of the ethyl acetate extract of the root resulted in the characterization of fifteen ellagitannins, among them punicalagin, castalagin, corilagin, tellimagrandin, terflavin B and S-flavogallonic acid, none of which have been identified before in C. hartmannianum. Six of the identified ellagitannins are unknown and NMR is needed to elucidate their molecular structures. Previously, flavogallonic acid dilactone and terchebulin have been identified in C. hartmannianum stem bark [3]. Our results indicate that roots of C. hartmannianum contain a high diversity and concentration of ellagitannins which could be potential agents against tuberculosis.

KW - 4112 Forestry

KW - 317 Pharmacy

UR - https://coms.events/GA2018/data/abstracts/en/abstract_0112.html

M3 - Poster

ER -

Salih EYA, Kanninen MT, Julkunen-Tiitto R, Sipi MH, Luukkanen MO, Vuorela HJ et al. Ellagitannins in antimycobacterial extracts of Combretum hartmannianum, a savannah woodland tree. 2018. Posterin esittämispaikka: 66th Annual Meeting of the Society for Medicinal Plant and Natural product research, Shanghai, Kiina.