Exposure in the Shoebox: Comparison of Physical Environment of IVCs and Open Rat Cages

Niina Kemppinen, Anna Meller, Erkki Bjork, Tarja Kohila, Timo Nevalainen

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

New caging and innovative items for more structured environment within the cage have been introduced. Many of these innovations cannot be seen as 'pure' or individual procedures, but rather they represent a mixed exposure with a multitude of operant factors, some possibly having an impact on animals and
research. One kind of new caging system is the individually ventilated cage (IVC), where each cage receives its own non-contaminated airflow, primarily designed for health status maintenance and occupational safety. Even though those cages may be the same as those used in open cage systems, the physical environment inside the cage may not identical. Comparison between cage types is difficult without characterization of the physical environment, because the change may involve alterations in several parameters in the environment. The aim of this study is to characterize and compare common physical parameters in the ordinary situation, where IVC-racks are kept in the same room with open cages. The cage type used was a polysulfone solid bottom cage. The parameters measured in this study were: illumination, temperature, relative humidity (RH) and acoustic level in both IVCs and open top cages. No animals were in the
cages during light intensity, but there was bedding in the cage during acoustic measurements and both bedding as well as a half-full food hopper during the illumination measurements. The temperature and (RH) measurements were carried out with three male rats in each cage. There were differences between IVCs and open top cages in all measured parameters. The light intensity was lower in IVCs, most likely due to more compact cage placement in the rack and the additional plastic cover lid of the cage. Both maximum and minimum temperatures were 1-4 oC higher in IVCs; which suggests that their ventilation is incapable of taking away heat, produced inside the cage. Similarly, the relative humidity was higher in the IVCs. The sound level adjusted to rat's hearing with R-weighting was higher in IVCs when compared to open cages. Furthermore, the sound level was highest in the corners next to the ventilation valves. In conclusion, there may be differences between open cages with IVCs involving several physical parameters of cage environment and this may confound comparisons between results obtained in these cage systems.
Alkuperäiskielienglanti
LehtiScandinavian Journal of Laboratory Animal Science
Vuosikerta35
Sivut97-103
Sivumäärä7
ISSN0901-3393
TilaJulkaistu - 2008
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 118 Biotieteet

Lainaa tätä

@article{6e5cdd64e3ea4b5aa99af3df3431981f,
title = "Exposure in the Shoebox: Comparison of Physical Environment of IVCs and Open Rat Cages",
abstract = "New caging and innovative items for more structured environment within the cage have been introduced. Many of these innovations cannot be seen as 'pure' or individual procedures, but rather they represent a mixed exposure with a multitude of operant factors, some possibly having an impact on animals andresearch. One kind of new caging system is the individually ventilated cage (IVC), where each cage receives its own non-contaminated airflow, primarily designed for health status maintenance and occupational safety. Even though those cages may be the same as those used in open cage systems, the physical environment inside the cage may not identical. Comparison between cage types is difficult without characterization of the physical environment, because the change may involve alterations in several parameters in the environment. The aim of this study is to characterize and compare common physical parameters in the ordinary situation, where IVC-racks are kept in the same room with open cages. The cage type used was a polysulfone solid bottom cage. The parameters measured in this study were: illumination, temperature, relative humidity (RH) and acoustic level in both IVCs and open top cages. No animals were in thecages during light intensity, but there was bedding in the cage during acoustic measurements and both bedding as well as a half-full food hopper during the illumination measurements. The temperature and (RH) measurements were carried out with three male rats in each cage. There were differences between IVCs and open top cages in all measured parameters. The light intensity was lower in IVCs, most likely due to more compact cage placement in the rack and the additional plastic cover lid of the cage. Both maximum and minimum temperatures were 1-4 oC higher in IVCs; which suggests that their ventilation is incapable of taking away heat, produced inside the cage. Similarly, the relative humidity was higher in the IVCs. The sound level adjusted to rat's hearing with R-weighting was higher in IVCs when compared to open cages. Furthermore, the sound level was highest in the corners next to the ventilation valves. In conclusion, there may be differences between open cages with IVCs involving several physical parameters of cage environment and this may confound comparisons between results obtained in these cage systems.",
keywords = "LABORATORY RODENTS, HEALTH-ASPECTS, SYSTEMS, 118 Biological sciences",
author = "Niina Kemppinen and Anna Meller and Erkki Bjork and Tarja Kohila and Timo Nevalainen",
year = "2008",
language = "English",
volume = "35",
pages = "97--103",
journal = "Scandinavian Journal of Laboratory Animal Science",
issn = "0901-3393",
publisher = "SCANDINAVIAN FEDERATION LABORATORY ANIMAL SCIENCE",

}

Exposure in the Shoebox: Comparison of Physical Environment of IVCs and Open Rat Cages. / Kemppinen, Niina; Meller, Anna; Bjork, Erkki; Kohila, Tarja; Nevalainen, Timo.

julkaisussa: Scandinavian Journal of Laboratory Animal Science, Vuosikerta 35, 2008, s. 97-103.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Exposure in the Shoebox: Comparison of Physical Environment of IVCs and Open Rat Cages

AU - Kemppinen, Niina

AU - Meller, Anna

AU - Bjork, Erkki

AU - Kohila, Tarja

AU - Nevalainen, Timo

PY - 2008

Y1 - 2008

N2 - New caging and innovative items for more structured environment within the cage have been introduced. Many of these innovations cannot be seen as 'pure' or individual procedures, but rather they represent a mixed exposure with a multitude of operant factors, some possibly having an impact on animals andresearch. One kind of new caging system is the individually ventilated cage (IVC), where each cage receives its own non-contaminated airflow, primarily designed for health status maintenance and occupational safety. Even though those cages may be the same as those used in open cage systems, the physical environment inside the cage may not identical. Comparison between cage types is difficult without characterization of the physical environment, because the change may involve alterations in several parameters in the environment. The aim of this study is to characterize and compare common physical parameters in the ordinary situation, where IVC-racks are kept in the same room with open cages. The cage type used was a polysulfone solid bottom cage. The parameters measured in this study were: illumination, temperature, relative humidity (RH) and acoustic level in both IVCs and open top cages. No animals were in thecages during light intensity, but there was bedding in the cage during acoustic measurements and both bedding as well as a half-full food hopper during the illumination measurements. The temperature and (RH) measurements were carried out with three male rats in each cage. There were differences between IVCs and open top cages in all measured parameters. The light intensity was lower in IVCs, most likely due to more compact cage placement in the rack and the additional plastic cover lid of the cage. Both maximum and minimum temperatures were 1-4 oC higher in IVCs; which suggests that their ventilation is incapable of taking away heat, produced inside the cage. Similarly, the relative humidity was higher in the IVCs. The sound level adjusted to rat's hearing with R-weighting was higher in IVCs when compared to open cages. Furthermore, the sound level was highest in the corners next to the ventilation valves. In conclusion, there may be differences between open cages with IVCs involving several physical parameters of cage environment and this may confound comparisons between results obtained in these cage systems.

AB - New caging and innovative items for more structured environment within the cage have been introduced. Many of these innovations cannot be seen as 'pure' or individual procedures, but rather they represent a mixed exposure with a multitude of operant factors, some possibly having an impact on animals andresearch. One kind of new caging system is the individually ventilated cage (IVC), where each cage receives its own non-contaminated airflow, primarily designed for health status maintenance and occupational safety. Even though those cages may be the same as those used in open cage systems, the physical environment inside the cage may not identical. Comparison between cage types is difficult without characterization of the physical environment, because the change may involve alterations in several parameters in the environment. The aim of this study is to characterize and compare common physical parameters in the ordinary situation, where IVC-racks are kept in the same room with open cages. The cage type used was a polysulfone solid bottom cage. The parameters measured in this study were: illumination, temperature, relative humidity (RH) and acoustic level in both IVCs and open top cages. No animals were in thecages during light intensity, but there was bedding in the cage during acoustic measurements and both bedding as well as a half-full food hopper during the illumination measurements. The temperature and (RH) measurements were carried out with three male rats in each cage. There were differences between IVCs and open top cages in all measured parameters. The light intensity was lower in IVCs, most likely due to more compact cage placement in the rack and the additional plastic cover lid of the cage. Both maximum and minimum temperatures were 1-4 oC higher in IVCs; which suggests that their ventilation is incapable of taking away heat, produced inside the cage. Similarly, the relative humidity was higher in the IVCs. The sound level adjusted to rat's hearing with R-weighting was higher in IVCs when compared to open cages. Furthermore, the sound level was highest in the corners next to the ventilation valves. In conclusion, there may be differences between open cages with IVCs involving several physical parameters of cage environment and this may confound comparisons between results obtained in these cage systems.

KW - LABORATORY RODENTS

KW - HEALTH-ASPECTS

KW - SYSTEMS

KW - 118 Biological sciences

M3 - Article

VL - 35

SP - 97

EP - 103

JO - Scandinavian Journal of Laboratory Animal Science

JF - Scandinavian Journal of Laboratory Animal Science

SN - 0901-3393

ER -