Fatty acids of lipid fractions in extracellular polymeric substancers of activated sludge flocs

Arnaud Conrad, Merja Kontro (Suutari), Minna M. Keinänen, Aurore Cadoret, Pierre Faure, Laurence Mansuy-Huault, Jean-Claude Block

    Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

    Kuvaus

    Phospholipid (PL), glycolipid (GL), and neutral lipid (NL) FA, and the lipopolysaccharide 2- and 3-hydroxy (LPS 2-OH and 3-OH) FA of activated sludges and extracted extracellular polymeric substances (EPS) were determined on samples collected from two wastewater treatment plants. EPS extracted from sludges by means of sonication and cation exchange contained proteins (43.4%), humic-like substances (11.5%), nucleic acids (10.9%), carbohydrates (9.9%), and lipid-bound FA (1.8%). The lipids associated with EPS were composed of GL, PL, NL, and LPS acids in proportions of 61, 21, 16, and 2%, respectively. The profiles of lipid-bound FA in activated sludges and EPS were similar (around 85 separate FA were identified). The FA signatures observed can be attributed to the likely presence of yeasts, fungi, sulfate-reducing bacteria, gram-positive and gram-negative bacteria, and, in lesser quantities, mycobacteria. Comparison of data from the dates of sampling (January and September) showed that there were more unsaturated PLFA in the EPS extracted from the activated sludges sampled in January. This observation could be partly related to microorganism adaptation to temperature variations. The comparison between two wastewater treatment plants showed that the FA profiles were similar, although differences in microbial community structure were also seen. Most of the FA in sludges had an even number of carbons.
    Alkuperäiskielienglanti
    LehtiLipids
    Vuosikerta38
    Numero10
    Sivut1093-1105
    Sivumäärä13
    ISSN0024-4201
    TilaJulkaistu - 2003
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

    Lainaa tätä

    Conrad, A., Kontro (Suutari), M., Keinänen, M. M., Cadoret, A., Faure, P., Mansuy-Huault, L., & Block, J-C. (2003). Fatty acids of lipid fractions in extracellular polymeric substancers of activated sludge flocs. Lipids, 38(10), 1093-1105.
    Conrad, Arnaud ; Kontro (Suutari), Merja ; Keinänen, Minna M. ; Cadoret, Aurore ; Faure, Pierre ; Mansuy-Huault, Laurence ; Block, Jean-Claude. / Fatty acids of lipid fractions in extracellular polymeric substancers of activated sludge flocs. Julkaisussa: Lipids. 2003 ; Vuosikerta 38, Nro 10. Sivut 1093-1105.
    @article{f467e82c4dda4567bed80e9ce14e0fc9,
    title = "Fatty acids of lipid fractions in extracellular polymeric substancers of activated sludge flocs",
    abstract = "Phospholipid (PL), glycolipid (GL), and neutral lipid (NL) FA, and the lipopolysaccharide 2- and 3-hydroxy (LPS 2-OH and 3-OH) FA of activated sludges and extracted extracellular polymeric substances (EPS) were determined on samples collected from two wastewater treatment plants. EPS extracted from sludges by means of sonication and cation exchange contained proteins (43.4{\%}), humic-like substances (11.5{\%}), nucleic acids (10.9{\%}), carbohydrates (9.9{\%}), and lipid-bound FA (1.8{\%}). The lipids associated with EPS were composed of GL, PL, NL, and LPS acids in proportions of 61, 21, 16, and 2{\%}, respectively. The profiles of lipid-bound FA in activated sludges and EPS were similar (around 85 separate FA were identified). The FA signatures observed can be attributed to the likely presence of yeasts, fungi, sulfate-reducing bacteria, gram-positive and gram-negative bacteria, and, in lesser quantities, mycobacteria. Comparison of data from the dates of sampling (January and September) showed that there were more unsaturated PLFA in the EPS extracted from the activated sludges sampled in January. This observation could be partly related to microorganism adaptation to temperature variations. The comparison between two wastewater treatment plants showed that the FA profiles were similar, although differences in microbial community structure were also seen. Most of the FA in sludges had an even number of carbons.",
    author = "Arnaud Conrad and {Kontro (Suutari)}, Merja and Kein{\"a}nen, {Minna M.} and Aurore Cadoret and Pierre Faure and Laurence Mansuy-Huault and Jean-Claude Block",
    year = "2003",
    language = "English",
    volume = "38",
    pages = "1093--1105",
    journal = "Lipids",
    issn = "0024-4201",
    publisher = "Wiley",
    number = "10",

    }

    Conrad, A, Kontro (Suutari), M, Keinänen, MM, Cadoret, A, Faure, P, Mansuy-Huault, L & Block, J-C 2003, 'Fatty acids of lipid fractions in extracellular polymeric substancers of activated sludge flocs', Lipids, Vuosikerta 38, Nro 10, Sivut 1093-1105.

    Fatty acids of lipid fractions in extracellular polymeric substancers of activated sludge flocs. / Conrad, Arnaud; Kontro (Suutari), Merja; Keinänen, Minna M.; Cadoret, Aurore; Faure, Pierre; Mansuy-Huault, Laurence; Block, Jean-Claude.

    julkaisussa: Lipids, Vuosikerta 38, Nro 10, 2003, s. 1093-1105.

    Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

    TY - JOUR

    T1 - Fatty acids of lipid fractions in extracellular polymeric substancers of activated sludge flocs

    AU - Conrad, Arnaud

    AU - Kontro (Suutari), Merja

    AU - Keinänen, Minna M.

    AU - Cadoret, Aurore

    AU - Faure, Pierre

    AU - Mansuy-Huault, Laurence

    AU - Block, Jean-Claude

    PY - 2003

    Y1 - 2003

    N2 - Phospholipid (PL), glycolipid (GL), and neutral lipid (NL) FA, and the lipopolysaccharide 2- and 3-hydroxy (LPS 2-OH and 3-OH) FA of activated sludges and extracted extracellular polymeric substances (EPS) were determined on samples collected from two wastewater treatment plants. EPS extracted from sludges by means of sonication and cation exchange contained proteins (43.4%), humic-like substances (11.5%), nucleic acids (10.9%), carbohydrates (9.9%), and lipid-bound FA (1.8%). The lipids associated with EPS were composed of GL, PL, NL, and LPS acids in proportions of 61, 21, 16, and 2%, respectively. The profiles of lipid-bound FA in activated sludges and EPS were similar (around 85 separate FA were identified). The FA signatures observed can be attributed to the likely presence of yeasts, fungi, sulfate-reducing bacteria, gram-positive and gram-negative bacteria, and, in lesser quantities, mycobacteria. Comparison of data from the dates of sampling (January and September) showed that there were more unsaturated PLFA in the EPS extracted from the activated sludges sampled in January. This observation could be partly related to microorganism adaptation to temperature variations. The comparison between two wastewater treatment plants showed that the FA profiles were similar, although differences in microbial community structure were also seen. Most of the FA in sludges had an even number of carbons.

    AB - Phospholipid (PL), glycolipid (GL), and neutral lipid (NL) FA, and the lipopolysaccharide 2- and 3-hydroxy (LPS 2-OH and 3-OH) FA of activated sludges and extracted extracellular polymeric substances (EPS) were determined on samples collected from two wastewater treatment plants. EPS extracted from sludges by means of sonication and cation exchange contained proteins (43.4%), humic-like substances (11.5%), nucleic acids (10.9%), carbohydrates (9.9%), and lipid-bound FA (1.8%). The lipids associated with EPS were composed of GL, PL, NL, and LPS acids in proportions of 61, 21, 16, and 2%, respectively. The profiles of lipid-bound FA in activated sludges and EPS were similar (around 85 separate FA were identified). The FA signatures observed can be attributed to the likely presence of yeasts, fungi, sulfate-reducing bacteria, gram-positive and gram-negative bacteria, and, in lesser quantities, mycobacteria. Comparison of data from the dates of sampling (January and September) showed that there were more unsaturated PLFA in the EPS extracted from the activated sludges sampled in January. This observation could be partly related to microorganism adaptation to temperature variations. The comparison between two wastewater treatment plants showed that the FA profiles were similar, although differences in microbial community structure were also seen. Most of the FA in sludges had an even number of carbons.

    M3 - Article

    VL - 38

    SP - 1093

    EP - 1105

    JO - Lipids

    JF - Lipids

    SN - 0024-4201

    IS - 10

    ER -

    Conrad A, Kontro (Suutari) M, Keinänen MM, Cadoret A, Faure P, Mansuy-Huault L et al. Fatty acids of lipid fractions in extracellular polymeric substancers of activated sludge flocs. Lipids. 2003;38(10):1093-1105.