Functional diversification of duplicated chalcone synthase genes in anthocyanin biosynthesis of Gerbera hybrida

Xianbao Deng, Hany Bashandy, Miia Ainasoja, Juha Kontturi, Milla Pietiäinen, Roosa Anna Emilia Laitinen, Victor A. Albert, Jari Valkonen, Paula Elomaa, Teemu Teeri

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Chalcone synthase (CHS) is the key enzyme in the first committed step of the flavonoid biosynthetic
pathway and catalyzes the stepwise condensation of 4-coumaroyl-CoA and malonyl-
CoA to naringenin chalcone. In plants, CHS is often encoded by a small family of genes
that are temporally and spatially regulated. Our earlier studies have shown that GCHS4 is
highly activated by ectopic expression of an MYB-type regulator GMYB10 in gerbera
(Gerbera hybrida).
The tissue- and development-specific expression patterns of three gerbera CHS genes were
examined. Virus-induced gene silencing (VIGS) was used to knock down GCHS1 and GCHS4
separately in gerbera inflorescences.
Our data show that GCHS4 is the only CHS encoding gene that is expressed in the cyanidin-
pigmented vegetative tissues of gerbera cv Terraregina. GCHS3 expression is pronounced
in the pappus bristles of the flowers. Expression of both GCHS1 and GCHS4 is high in the epidermal
cells of gerbera petals, but only GCHS1 is contributing to flavonoid biosynthesis.
Gerbera contains a family of three CHS encoding genes showing different spatial and temporal
regulation. GCHS4 expression in gerbera petals is regulated post-transcriptionally, at the
level of either translation elongation or protein stability
Alkuperäiskielienglanti
LehtiNew Phytologist
Vuosikerta201
Numero4
Sivut1469-1483
Sivumäärä15
ISSN0028-646X
DOI - pysyväislinkit
TilaJulkaistu - 2014
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 414 Maatalouden bioteknologia

Lainaa tätä

Deng, Xianbao ; Bashandy, Hany ; Ainasoja, Miia ; Kontturi, Juha ; Pietiäinen, Milla ; Laitinen, Roosa Anna Emilia ; Albert, Victor A. ; Valkonen, Jari ; Elomaa, Paula ; Teeri, Teemu. / Functional diversification of duplicated chalcone synthase genes in anthocyanin biosynthesis of Gerbera hybrida. Julkaisussa: New Phytologist. 2014 ; Vuosikerta 201, Nro 4. Sivut 1469-1483.
@article{a3d76d904a324ffcbb3d7a0d61073412,
title = "Functional diversification of duplicated chalcone synthase genes in anthocyanin biosynthesis of Gerbera hybrida",
abstract = "Chalcone synthase (CHS) is the key enzyme in the first committed step of the flavonoid biosyntheticpathway and catalyzes the stepwise condensation of 4-coumaroyl-CoA and malonyl-CoA to naringenin chalcone. In plants, CHS is often encoded by a small family of genesthat are temporally and spatially regulated. Our earlier studies have shown that GCHS4 ishighly activated by ectopic expression of an MYB-type regulator GMYB10 in gerbera(Gerbera hybrida). The tissue- and development-specific expression patterns of three gerbera CHS genes wereexamined. Virus-induced gene silencing (VIGS) was used to knock down GCHS1 and GCHS4separately in gerbera inflorescences. Our data show that GCHS4 is the only CHS encoding gene that is expressed in the cyanidin-pigmented vegetative tissues of gerbera cv Terraregina. GCHS3 expression is pronouncedin the pappus bristles of the flowers. Expression of both GCHS1 and GCHS4 is high in the epidermalcells of gerbera petals, but only GCHS1 is contributing to flavonoid biosynthesis. Gerbera contains a family of three CHS encoding genes showing different spatial and temporalregulation. GCHS4 expression in gerbera petals is regulated post-transcriptionally, at thelevel of either translation elongation or protein stability",
keywords = "414 Agricultural biotechnology, anthocyanin, Asteraceae, chalcone synthase, flavonoid, Gerbera hybrida.",
author = "Xianbao Deng and Hany Bashandy and Miia Ainasoja and Juha Kontturi and Milla Pieti{\"a}inen and Laitinen, {Roosa Anna Emilia} and Albert, {Victor A.} and Jari Valkonen and Paula Elomaa and Teemu Teeri",
year = "2014",
doi = "10.1111/nph.12610",
language = "English",
volume = "201",
pages = "1469--1483",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "4",

}

Functional diversification of duplicated chalcone synthase genes in anthocyanin biosynthesis of Gerbera hybrida. / Deng, Xianbao; Bashandy, Hany; Ainasoja, Miia; Kontturi, Juha; Pietiäinen, Milla; Laitinen, Roosa Anna Emilia; Albert, Victor A.; Valkonen, Jari; Elomaa, Paula; Teeri, Teemu.

julkaisussa: New Phytologist, Vuosikerta 201, Nro 4, 2014, s. 1469-1483.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Functional diversification of duplicated chalcone synthase genes in anthocyanin biosynthesis of Gerbera hybrida

AU - Deng, Xianbao

AU - Bashandy, Hany

AU - Ainasoja, Miia

AU - Kontturi, Juha

AU - Pietiäinen, Milla

AU - Laitinen, Roosa Anna Emilia

AU - Albert, Victor A.

AU - Valkonen, Jari

AU - Elomaa, Paula

AU - Teeri, Teemu

PY - 2014

Y1 - 2014

N2 - Chalcone synthase (CHS) is the key enzyme in the first committed step of the flavonoid biosyntheticpathway and catalyzes the stepwise condensation of 4-coumaroyl-CoA and malonyl-CoA to naringenin chalcone. In plants, CHS is often encoded by a small family of genesthat are temporally and spatially regulated. Our earlier studies have shown that GCHS4 ishighly activated by ectopic expression of an MYB-type regulator GMYB10 in gerbera(Gerbera hybrida). The tissue- and development-specific expression patterns of three gerbera CHS genes wereexamined. Virus-induced gene silencing (VIGS) was used to knock down GCHS1 and GCHS4separately in gerbera inflorescences. Our data show that GCHS4 is the only CHS encoding gene that is expressed in the cyanidin-pigmented vegetative tissues of gerbera cv Terraregina. GCHS3 expression is pronouncedin the pappus bristles of the flowers. Expression of both GCHS1 and GCHS4 is high in the epidermalcells of gerbera petals, but only GCHS1 is contributing to flavonoid biosynthesis. Gerbera contains a family of three CHS encoding genes showing different spatial and temporalregulation. GCHS4 expression in gerbera petals is regulated post-transcriptionally, at thelevel of either translation elongation or protein stability

AB - Chalcone synthase (CHS) is the key enzyme in the first committed step of the flavonoid biosyntheticpathway and catalyzes the stepwise condensation of 4-coumaroyl-CoA and malonyl-CoA to naringenin chalcone. In plants, CHS is often encoded by a small family of genesthat are temporally and spatially regulated. Our earlier studies have shown that GCHS4 ishighly activated by ectopic expression of an MYB-type regulator GMYB10 in gerbera(Gerbera hybrida). The tissue- and development-specific expression patterns of three gerbera CHS genes wereexamined. Virus-induced gene silencing (VIGS) was used to knock down GCHS1 and GCHS4separately in gerbera inflorescences. Our data show that GCHS4 is the only CHS encoding gene that is expressed in the cyanidin-pigmented vegetative tissues of gerbera cv Terraregina. GCHS3 expression is pronouncedin the pappus bristles of the flowers. Expression of both GCHS1 and GCHS4 is high in the epidermalcells of gerbera petals, but only GCHS1 is contributing to flavonoid biosynthesis. Gerbera contains a family of three CHS encoding genes showing different spatial and temporalregulation. GCHS4 expression in gerbera petals is regulated post-transcriptionally, at thelevel of either translation elongation or protein stability

KW - 414 Agricultural biotechnology

KW - anthocyanin, Asteraceae, chalcone synthase, flavonoid, Gerbera hybrida.

U2 - 10.1111/nph.12610

DO - 10.1111/nph.12610

M3 - Article

VL - 201

SP - 1469

EP - 1483

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 4

ER -