Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria.

Pirjo Torkko, Marja-Leena Katila, Merja Kontro

    Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

    Kuvaus

    Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combiningmycolic acid cleavage productswith shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environmental species. In this scheme, the species are classified into six categories, each characterized by a combination of fatty markers shared by those species. Within each category, individual species may be distinguished by the presence or absence of specific marker substances, such as methyl-branched fatty acids or secondary alcohols. This study also describes earlier unpublished GLC profiles of 14 rare, slowly growing, environmental mycobacteria, Mycobacterium asiaticum, Mycobacterium botniense, Mycobacterium branderi, Mycobacterium conspicuum, Mycobacteriumcookii,Mycobacteriumdoricum,Mycobacteriumheckeshornense,Mycobacterium heidelbergense, Mycobacterium hiberniae, Mycobacterium kubicae, Mycobacterium lentiflavum, Mycobacterium scrofulaceum, Mycobacterium triplex and Mycobacterium tusciae. Though no single identification technique alone, even sequencing of an entire single gene such as 16S rRNA, can identify all mycobacterial species accurately, GLC has proven to be both reliable and reproducible in the identification of slowly growing mycobacteria. In cases of earlier unknown species, it generates useful information that allows their further classification and may lead to the description of novel species.
    Alkuperäiskielienglanti
    LehtiJournal of Medical Microbiology
    Vuosikerta52
    Sivut315-323
    Sivumäärä9
    ISSN0022-2615
    TilaJulkaistu - 2003
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

    Lainaa tätä

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    title = "Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria.",
    abstract = "Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combiningmycolic acid cleavage productswith shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environmental species. In this scheme, the species are classified into six categories, each characterized by a combination of fatty markers shared by those species. Within each category, individual species may be distinguished by the presence or absence of specific marker substances, such as methyl-branched fatty acids or secondary alcohols. This study also describes earlier unpublished GLC profiles of 14 rare, slowly growing, environmental mycobacteria, Mycobacterium asiaticum, Mycobacterium botniense, Mycobacterium branderi, Mycobacterium conspicuum, Mycobacteriumcookii,Mycobacteriumdoricum,Mycobacteriumheckeshornense,Mycobacterium heidelbergense, Mycobacterium hiberniae, Mycobacterium kubicae, Mycobacterium lentiflavum, Mycobacterium scrofulaceum, Mycobacterium triplex and Mycobacterium tusciae. Though no single identification technique alone, even sequencing of an entire single gene such as 16S rRNA, can identify all mycobacterial species accurately, GLC has proven to be both reliable and reproducible in the identification of slowly growing mycobacteria. In cases of earlier unknown species, it generates useful information that allows their further classification and may lead to the description of novel species.",
    author = "Pirjo Torkko and Marja-Leena Katila and Merja Kontro",
    year = "2003",
    language = "English",
    volume = "52",
    pages = "315--323",
    journal = "Journal of Medical Microbiology",
    issn = "0022-2615",
    publisher = "SOCIETY FOR GENERAL MICROBIOLOGY",

    }

    Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria. / Torkko, Pirjo; Katila, Marja-Leena; Kontro, Merja.

    julkaisussa: Journal of Medical Microbiology, Vuosikerta 52, 2003, s. 315-323.

    Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

    TY - JOUR

    T1 - Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria.

    AU - Torkko, Pirjo

    AU - Katila, Marja-Leena

    AU - Kontro, Merja

    PY - 2003

    Y1 - 2003

    N2 - Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combiningmycolic acid cleavage productswith shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environmental species. In this scheme, the species are classified into six categories, each characterized by a combination of fatty markers shared by those species. Within each category, individual species may be distinguished by the presence or absence of specific marker substances, such as methyl-branched fatty acids or secondary alcohols. This study also describes earlier unpublished GLC profiles of 14 rare, slowly growing, environmental mycobacteria, Mycobacterium asiaticum, Mycobacterium botniense, Mycobacterium branderi, Mycobacterium conspicuum, Mycobacteriumcookii,Mycobacteriumdoricum,Mycobacteriumheckeshornense,Mycobacterium heidelbergense, Mycobacterium hiberniae, Mycobacterium kubicae, Mycobacterium lentiflavum, Mycobacterium scrofulaceum, Mycobacterium triplex and Mycobacterium tusciae. Though no single identification technique alone, even sequencing of an entire single gene such as 16S rRNA, can identify all mycobacterial species accurately, GLC has proven to be both reliable and reproducible in the identification of slowly growing mycobacteria. In cases of earlier unknown species, it generates useful information that allows their further classification and may lead to the description of novel species.

    AB - Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combiningmycolic acid cleavage productswith shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environmental species. In this scheme, the species are classified into six categories, each characterized by a combination of fatty markers shared by those species. Within each category, individual species may be distinguished by the presence or absence of specific marker substances, such as methyl-branched fatty acids or secondary alcohols. This study also describes earlier unpublished GLC profiles of 14 rare, slowly growing, environmental mycobacteria, Mycobacterium asiaticum, Mycobacterium botniense, Mycobacterium branderi, Mycobacterium conspicuum, Mycobacteriumcookii,Mycobacteriumdoricum,Mycobacteriumheckeshornense,Mycobacterium heidelbergense, Mycobacterium hiberniae, Mycobacterium kubicae, Mycobacterium lentiflavum, Mycobacterium scrofulaceum, Mycobacterium triplex and Mycobacterium tusciae. Though no single identification technique alone, even sequencing of an entire single gene such as 16S rRNA, can identify all mycobacterial species accurately, GLC has proven to be both reliable and reproducible in the identification of slowly growing mycobacteria. In cases of earlier unknown species, it generates useful information that allows their further classification and may lead to the description of novel species.

    M3 - Article

    VL - 52

    SP - 315

    EP - 323

    JO - Journal of Medical Microbiology

    JF - Journal of Medical Microbiology

    SN - 0022-2615

    ER -