Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm

M.M. Keinänen, L.L. Korhonen, P.J. Martikainen, T Vartiainen, I.T. Miettinen, M.J. Lehtola, K Nenonen, H. Pajunen, Merja H. Kontro

    Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

    Kuvaus

    Hydroxy fatty acids (OH-FAs) can be used in the characterization of microbial communities, especially Gram-negative bacteria. We prepared methyl esters of 2- and 3-OH-FAs from the lipid extraction residue of soil, sediment, and biofilm samples without further purification or derivatization of hydroxyl groups. OH-FA methyl esters were analyzed using a gas chromatograph equipped with a mass selective detector (GC–MS). The ions followed in MS were m/z 103 for 3-OH-FAs and m/z 90 and M-59 for 2-OH-FAs. The rapid determination of 3- and 2-OH-FAs concomitantly with phospholipid fatty acids provided more detailed information on the microbial communities present in soil, sediment, and drinking water biofilm.
    Alkuperäiskielienglanti
    LehtiJournal of Chromatography. A
    Vuosikerta783
    Sivut443-451
    Sivumäärä9
    ISSN0021-9673
    TilaJulkaistu - 2003
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

    Lainaa tätä

    Keinänen, M. M., Korhonen, L. L., Martikainen, P. J., Vartiainen, T., Miettinen, I. T., Lehtola, M. J., ... Kontro, M. H. (2003). Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm. Journal of Chromatography. A, 783, 443-451.
    Keinänen, M.M. ; Korhonen, L.L. ; Martikainen, P.J. ; Vartiainen, T ; Miettinen, I.T. ; Lehtola, M.J. ; Nenonen, K ; Pajunen, H. ; Kontro, Merja H. / Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm. Julkaisussa: Journal of Chromatography. A. 2003 ; Vuosikerta 783. Sivut 443-451.
    @article{1330f391398f447b8def0fb80cc04da2,
    title = "Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm",
    abstract = "Hydroxy fatty acids (OH-FAs) can be used in the characterization of microbial communities, especially Gram-negative bacteria. We prepared methyl esters of 2- and 3-OH-FAs from the lipid extraction residue of soil, sediment, and biofilm samples without further purification or derivatization of hydroxyl groups. OH-FA methyl esters were analyzed using a gas chromatograph equipped with a mass selective detector (GC–MS). The ions followed in MS were m/z 103 for 3-OH-FAs and m/z 90 and M-59 for 2-OH-FAs. The rapid determination of 3- and 2-OH-FAs concomitantly with phospholipid fatty acids provided more detailed information on the microbial communities present in soil, sediment, and drinking water biofilm.",
    author = "M.M. Kein{\"a}nen and L.L. Korhonen and P.J. Martikainen and T Vartiainen and I.T. Miettinen and M.J. Lehtola and K Nenonen and H. Pajunen and Kontro, {Merja H.}",
    year = "2003",
    language = "English",
    volume = "783",
    pages = "443--451",
    journal = "Journal of Chromatography. A",
    issn = "0021-9673",
    publisher = "Elsevier Scientific Publ. Co",

    }

    Keinänen, MM, Korhonen, LL, Martikainen, PJ, Vartiainen, T, Miettinen, IT, Lehtola, MJ, Nenonen, K, Pajunen, H & Kontro, MH 2003, 'Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm', Journal of Chromatography. A, Vuosikerta 783, Sivut 443-451.

    Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm. / Keinänen, M.M.; Korhonen, L.L.; Martikainen, P.J.; Vartiainen, T; Miettinen, I.T.; Lehtola, M.J.; Nenonen, K; Pajunen, H.; Kontro, Merja H.

    julkaisussa: Journal of Chromatography. A, Vuosikerta 783, 2003, s. 443-451.

    Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

    TY - JOUR

    T1 - Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm

    AU - Keinänen, M.M.

    AU - Korhonen, L.L.

    AU - Martikainen, P.J.

    AU - Vartiainen, T

    AU - Miettinen, I.T.

    AU - Lehtola, M.J.

    AU - Nenonen, K

    AU - Pajunen, H.

    AU - Kontro, Merja H.

    PY - 2003

    Y1 - 2003

    N2 - Hydroxy fatty acids (OH-FAs) can be used in the characterization of microbial communities, especially Gram-negative bacteria. We prepared methyl esters of 2- and 3-OH-FAs from the lipid extraction residue of soil, sediment, and biofilm samples without further purification or derivatization of hydroxyl groups. OH-FA methyl esters were analyzed using a gas chromatograph equipped with a mass selective detector (GC–MS). The ions followed in MS were m/z 103 for 3-OH-FAs and m/z 90 and M-59 for 2-OH-FAs. The rapid determination of 3- and 2-OH-FAs concomitantly with phospholipid fatty acids provided more detailed information on the microbial communities present in soil, sediment, and drinking water biofilm.

    AB - Hydroxy fatty acids (OH-FAs) can be used in the characterization of microbial communities, especially Gram-negative bacteria. We prepared methyl esters of 2- and 3-OH-FAs from the lipid extraction residue of soil, sediment, and biofilm samples without further purification or derivatization of hydroxyl groups. OH-FA methyl esters were analyzed using a gas chromatograph equipped with a mass selective detector (GC–MS). The ions followed in MS were m/z 103 for 3-OH-FAs and m/z 90 and M-59 for 2-OH-FAs. The rapid determination of 3- and 2-OH-FAs concomitantly with phospholipid fatty acids provided more detailed information on the microbial communities present in soil, sediment, and drinking water biofilm.

    M3 - Article

    VL - 783

    SP - 443

    EP - 451

    JO - Journal of Chromatography. A

    JF - Journal of Chromatography. A

    SN - 0021-9673

    ER -

    Keinänen MM, Korhonen LL, Martikainen PJ, Vartiainen T, Miettinen IT, Lehtola MJ et al. Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm. Journal of Chromatography. A. 2003;783:443-451.