Genetic structure and marker-trait associations in parental lines of sunflower (Helianthus annuus L.)

Hossein Zeinakzadeh-Tabrizi, Arash Hosseinpour, Mehdi Ghaffari, Kamil Haliloglu, Alireza Pour-Aboughadareh, Péter Poczai

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Abstrakti

Association mapping (AM) is a reliable tool to examine the association between phenotypic characters and molecular markers based on linkage disequilibrium (LD). In this study, two simple sequence repeat (SSR) and target region amplification polymorphism (TRAP) molecular techniques were applied to investigate the genetic structure and association analysis in 53 Iranian sunflower parental lines, including 23 restorer and 30 cytoplasmic male sterility (CMS) lines. In the phenotypic section, 18 morphophysiological characters were measured. The maximum heritability was estimated for leaf number (96.67%), followed by seed yield (88.07%) and oil yield (87.20%). The results of STRUCTURE analysis, based on 21 SSR and 19 TRAP, indicated that the actual subpopulation (optimum K) was five and two, respectively. Population differentiation measurements (Fst) between the subpopulations ranged from 0.24 to 0.43, with an average of 0.32 in SSR data, while Fst value in the TRAP marker ranged from 0.23 to 0.24, with an average of 0.24. According to SSR and TRAP markers, of the 18 studied traits, significant marker-trait associations (P≤0.05) were found for 11 and 17 traits, respectively. SSR loci Ha 494-ar, Ha 806-ar, Ha 991-ar, Ha 1167-ar, Ha 1287-ar, ORS-53, and ORS-54 were associated with seed yield per plant, oil yield per plant, seed yield, and oil yield across three years, respectively. On the other hand, several TRAP markers, including K11F05/TRAP03, K11F05/TRAP03, and F15O11F1/TRAP03, were associated with flowering duration, maturity, and 1000-seed weight, respectively. In conclusion, the genetic structure and marker-trait associations reported here can be exploited for marker-assisted selection in sunflower breeding programs. Further validation in independent accession panels or bi-parental populations is needed.
Alkuperäiskielienglanti
LehtiEuphytica
ISSN0014-2336
TilaJätetty - 2020
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

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