Immunochemical analysis of oat prolamins in European oat cultivars

Tutkimustuotos: KonferenssimateriaalitPosteri

Kuvaus

Celiac disease (CD) is a multisystem immune based disorder. Ingested gluten triggers a response that can lead to severe gastrointestinal symptoms, metabolic bone disease and/or infertility. The only current treatment is a strict gluten free diet. There is a compelling amount of evidence, including a number of clinical trials in Finland and Northern Europe, that oats can be a suitable and palatable part of a gluten free diet for both children and adult CD patients. Oats also have other properties of special interest, such as high content of dietary fibre, which in itself holds several scientifically approved nutritional health claims. However, there remains room for debate whether all oat cultivars are safe for CD patients. This why variations in protein composition and immunogenicity of avenins need to be further investigated before further clinical tests.
In the pursuit of improving current gluten-free analytics, 26 European oat cultivars have been characterised by protein content and immunological affinity. Chosen oat cultivars include both commercial lines and landraces (e.g. Finnish, Swedish, Greek, Italian, Dutch). Some of the cultivars have been recorded to possess gluten-like activity in immunological assays as well as activating T-cells in vitro. Oat seeds were grown in regulated growing chambers. Each cultivar was hand dehulled and ground to flour using a small coffee mill dedicated to oats. These precautions, among others, were taken to ensure samples remained uncontaminated. Total protein content of cultivars varied between 15 and 24%. After extraction with aqueous alcohol solution, cultivars were characterised and grouped based on qualitative SDS-PAGE avenin profiles. The immunological reactivity of the avenins from 26 cultivars towards R5 antibody, G12 antibody and polyclonal gliadin antibody were investigated. Avenins were recognised by polyclonal gliadin antibody, while other proteins co-extracted in the aqueous alcohol solution were not. None of the cultivars was recognised by R5 Sandwich ELISA (enzyme-linked immunosorbent assay) and western blot, indicating the epitope QQPFP, which repeatedly appeared in Triticeae prolamins, is not presented in oat avenins. This study has started the characterisation of oat prolamins. The gluten free status of oats needs to be clarified for the safe consumption of oats by celiac disease patients.
Alkuperäiskielienglanti
TilaJulkaistu - kesäkuuta 2019
OKM-julkaisutyyppiEi sovellu
Tapahtuma5th International Symposium on Gluten-Free Cereal Products and Beverages 2019: Building products by knowledge - KU Leuven, Leuven, Belgia
Kesto: 26 kesäkuuta 2019 → …
http://www.gf19.icc.or.at/en/#

Konferenssi

Konferenssi5th International Symposium on Gluten-Free Cereal Products and Beverages 2019
LyhennettäGF19
MaaBelgia
KaupunkiLeuven
Ajanjakso26/06/2019 → …
www-osoite

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  • 416 Elintarviketieteet

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Ahola, H., Huang, X., & Sontag-Strohm, T. (2019). Immunochemical analysis of oat prolamins in European oat cultivars. Posterin esittämispaikka: 5th International Symposium on Gluten-Free Cereal Products and Beverages 2019, Leuven, Belgia.
Ahola, Hanna ; Huang, Xin ; Sontag-Strohm, Tuula. / Immunochemical analysis of oat prolamins in European oat cultivars. Posterin esittämispaikka: 5th International Symposium on Gluten-Free Cereal Products and Beverages 2019, Leuven, Belgia.
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title = "Immunochemical analysis of oat prolamins in European oat cultivars",
abstract = "Celiac disease (CD) is a multisystem immune based disorder. Ingested gluten triggers a response that can lead to severe gastrointestinal symptoms, metabolic bone disease and/or infertility. The only current treatment is a strict gluten free diet. There is a compelling amount of evidence, including a number of clinical trials in Finland and Northern Europe, that oats can be a suitable and palatable part of a gluten free diet for both children and adult CD patients. Oats also have other properties of special interest, such as high content of dietary fibre, which in itself holds several scientifically approved nutritional health claims. However, there remains room for debate whether all oat cultivars are safe for CD patients. This why variations in protein composition and immunogenicity of avenins need to be further investigated before further clinical tests. In the pursuit of improving current gluten-free analytics, 26 European oat cultivars have been characterised by protein content and immunological affinity. Chosen oat cultivars include both commercial lines and landraces (e.g. Finnish, Swedish, Greek, Italian, Dutch). Some of the cultivars have been recorded to possess gluten-like activity in immunological assays as well as activating T-cells in vitro. Oat seeds were grown in regulated growing chambers. Each cultivar was hand dehulled and ground to flour using a small coffee mill dedicated to oats. These precautions, among others, were taken to ensure samples remained uncontaminated. Total protein content of cultivars varied between 15 and 24{\%}. After extraction with aqueous alcohol solution, cultivars were characterised and grouped based on qualitative SDS-PAGE avenin profiles. The immunological reactivity of the avenins from 26 cultivars towards R5 antibody, G12 antibody and polyclonal gliadin antibody were investigated. Avenins were recognised by polyclonal gliadin antibody, while other proteins co-extracted in the aqueous alcohol solution were not. None of the cultivars was recognised by R5 Sandwich ELISA (enzyme-linked immunosorbent assay) and western blot, indicating the epitope QQPFP, which repeatedly appeared in Triticeae prolamins, is not presented in oat avenins. This study has started the characterisation of oat prolamins. The gluten free status of oats needs to be clarified for the safe consumption of oats by celiac disease patients.",
keywords = "416 Food Science, R5, ELISA, avenins, gluten-free",
author = "Hanna Ahola and Xin Huang and Tuula Sontag-Strohm",
year = "2019",
month = "6",
language = "English",
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Ahola, H, Huang, X & Sontag-Strohm, T 2019, 'Immunochemical analysis of oat prolamins in European oat cultivars', 5th International Symposium on Gluten-Free Cereal Products and Beverages 2019, Leuven, Belgia, 26/06/2019.

Immunochemical analysis of oat prolamins in European oat cultivars. / Ahola, Hanna; Huang, Xin; Sontag-Strohm, Tuula.

2019. Posterin esittämispaikka: 5th International Symposium on Gluten-Free Cereal Products and Beverages 2019, Leuven, Belgia.

Tutkimustuotos: KonferenssimateriaalitPosteri

TY - CONF

T1 - Immunochemical analysis of oat prolamins in European oat cultivars

AU - Ahola, Hanna

AU - Huang, Xin

AU - Sontag-Strohm, Tuula

PY - 2019/6

Y1 - 2019/6

N2 - Celiac disease (CD) is a multisystem immune based disorder. Ingested gluten triggers a response that can lead to severe gastrointestinal symptoms, metabolic bone disease and/or infertility. The only current treatment is a strict gluten free diet. There is a compelling amount of evidence, including a number of clinical trials in Finland and Northern Europe, that oats can be a suitable and palatable part of a gluten free diet for both children and adult CD patients. Oats also have other properties of special interest, such as high content of dietary fibre, which in itself holds several scientifically approved nutritional health claims. However, there remains room for debate whether all oat cultivars are safe for CD patients. This why variations in protein composition and immunogenicity of avenins need to be further investigated before further clinical tests. In the pursuit of improving current gluten-free analytics, 26 European oat cultivars have been characterised by protein content and immunological affinity. Chosen oat cultivars include both commercial lines and landraces (e.g. Finnish, Swedish, Greek, Italian, Dutch). Some of the cultivars have been recorded to possess gluten-like activity in immunological assays as well as activating T-cells in vitro. Oat seeds were grown in regulated growing chambers. Each cultivar was hand dehulled and ground to flour using a small coffee mill dedicated to oats. These precautions, among others, were taken to ensure samples remained uncontaminated. Total protein content of cultivars varied between 15 and 24%. After extraction with aqueous alcohol solution, cultivars were characterised and grouped based on qualitative SDS-PAGE avenin profiles. The immunological reactivity of the avenins from 26 cultivars towards R5 antibody, G12 antibody and polyclonal gliadin antibody were investigated. Avenins were recognised by polyclonal gliadin antibody, while other proteins co-extracted in the aqueous alcohol solution were not. None of the cultivars was recognised by R5 Sandwich ELISA (enzyme-linked immunosorbent assay) and western blot, indicating the epitope QQPFP, which repeatedly appeared in Triticeae prolamins, is not presented in oat avenins. This study has started the characterisation of oat prolamins. The gluten free status of oats needs to be clarified for the safe consumption of oats by celiac disease patients.

AB - Celiac disease (CD) is a multisystem immune based disorder. Ingested gluten triggers a response that can lead to severe gastrointestinal symptoms, metabolic bone disease and/or infertility. The only current treatment is a strict gluten free diet. There is a compelling amount of evidence, including a number of clinical trials in Finland and Northern Europe, that oats can be a suitable and palatable part of a gluten free diet for both children and adult CD patients. Oats also have other properties of special interest, such as high content of dietary fibre, which in itself holds several scientifically approved nutritional health claims. However, there remains room for debate whether all oat cultivars are safe for CD patients. This why variations in protein composition and immunogenicity of avenins need to be further investigated before further clinical tests. In the pursuit of improving current gluten-free analytics, 26 European oat cultivars have been characterised by protein content and immunological affinity. Chosen oat cultivars include both commercial lines and landraces (e.g. Finnish, Swedish, Greek, Italian, Dutch). Some of the cultivars have been recorded to possess gluten-like activity in immunological assays as well as activating T-cells in vitro. Oat seeds were grown in regulated growing chambers. Each cultivar was hand dehulled and ground to flour using a small coffee mill dedicated to oats. These precautions, among others, were taken to ensure samples remained uncontaminated. Total protein content of cultivars varied between 15 and 24%. After extraction with aqueous alcohol solution, cultivars were characterised and grouped based on qualitative SDS-PAGE avenin profiles. The immunological reactivity of the avenins from 26 cultivars towards R5 antibody, G12 antibody and polyclonal gliadin antibody were investigated. Avenins were recognised by polyclonal gliadin antibody, while other proteins co-extracted in the aqueous alcohol solution were not. None of the cultivars was recognised by R5 Sandwich ELISA (enzyme-linked immunosorbent assay) and western blot, indicating the epitope QQPFP, which repeatedly appeared in Triticeae prolamins, is not presented in oat avenins. This study has started the characterisation of oat prolamins. The gluten free status of oats needs to be clarified for the safe consumption of oats by celiac disease patients.

KW - 416 Food Science

KW - R5

KW - ELISA

KW - avenins

KW - gluten-free

M3 - Poster

ER -

Ahola H, Huang X, Sontag-Strohm T. Immunochemical analysis of oat prolamins in European oat cultivars. 2019. Posterin esittämispaikka: 5th International Symposium on Gluten-Free Cereal Products and Beverages 2019, Leuven, Belgia.