In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography

I. Kestilä, J. Thevenot, M. A. Finnilä, S. S. Karhula, I. Hadjab, S. Kauppinen, M. Garon, E. Quenneville, M. Haapea, L. Rieppo, K. P. Pritzker, M. D. Buschmann, H. J. Nieminen, S. Saarakkala

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Objective: The aims of this study were: to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (mu CT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC.

Method: The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop mCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semiautomatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1: 0-10%; zone 2: 10-40%; zone 3: 40-100%) and grouped by the OARSI histological grades (OARSI grades 0-1.0, n = 6; OARSI grades 3.0-3.5, n = 6).

Results: After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (P

Conclusion: We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/ chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA). (c) 2018 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Alkuperäiskielienglanti
LehtiOsteoarthritis and Cartilage
Vuosikerta26
Numero8
Sivut1118-1126
Sivumäärä9
ISSN1063-4584
DOI - pysyväislinkit
TilaJulkaistu - elokuuta 2018
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

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  • 217 Lääketieteen tekniikka

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Kestilä, I., Thevenot, J., Finnilä, M. A., Karhula, S. S., Hadjab, I., Kauppinen, S., ... Saarakkala, S. (2018). In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography. Osteoarthritis and Cartilage, 26(8), 1118-1126. https://doi.org/10.1016/j.joca.2018.05.012
Kestilä, I. ; Thevenot, J. ; Finnilä, M. A. ; Karhula, S. S. ; Hadjab, I. ; Kauppinen, S. ; Garon, M. ; Quenneville, E. ; Haapea, M. ; Rieppo, L. ; Pritzker, K. P. ; Buschmann, M. D. ; Nieminen, H. J. ; Saarakkala, S. / In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography. Julkaisussa: Osteoarthritis and Cartilage. 2018 ; Vuosikerta 26, Nro 8. Sivut 1118-1126.
@article{d6be6941d8884e1e809df0acddf33454,
title = "In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography",
abstract = "Objective: The aims of this study were: to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (mu CT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC.Method: The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop mCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semiautomatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1: 0-10{\%}; zone 2: 10-40{\%}; zone 3: 40-100{\%}) and grouped by the OARSI histological grades (OARSI grades 0-1.0, n = 6; OARSI grades 3.0-3.5, n = 6).Results: After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (PConclusion: We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/ chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA). (c) 2018 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).",
keywords = "Hexamethyldisilazane, Osteoarthritis, Segmentation, Morphology, 3D analysis, SCANNING ELECTRON-MICROSCOPY, ADULT HUMAN KNEE, 3-DIMENSIONAL MORPHOLOGY, CONFOCAL MICROSCOPY, PERICELLULAR MATRIX, CELL, OSTEOARTHRITIS, HEXAMETHYLDISILAZANE, MICROENVIRONMENT, ORGANIZATION, 217 Medical engineering",
author = "I. Kestil{\"a} and J. Thevenot and Finnil{\"a}, {M. A.} and Karhula, {S. S.} and I. Hadjab and S. Kauppinen and M. Garon and E. Quenneville and M. Haapea and L. Rieppo and Pritzker, {K. P.} and Buschmann, {M. D.} and Nieminen, {H. J.} and S. Saarakkala",
year = "2018",
month = "8",
doi = "10.1016/j.joca.2018.05.012",
language = "English",
volume = "26",
pages = "1118--1126",
journal = "Osteoarthritis and Cartilage",
issn = "1063-4584",
publisher = "ELSEVIER SCI IRELAND LTD",
number = "8",

}

Kestilä, I, Thevenot, J, Finnilä, MA, Karhula, SS, Hadjab, I, Kauppinen, S, Garon, M, Quenneville, E, Haapea, M, Rieppo, L, Pritzker, KP, Buschmann, MD, Nieminen, HJ & Saarakkala, S 2018, 'In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography', Osteoarthritis and Cartilage, Vuosikerta 26, Nro 8, Sivut 1118-1126. https://doi.org/10.1016/j.joca.2018.05.012

In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography. / Kestilä, I.; Thevenot, J.; Finnilä, M. A.; Karhula, S. S.; Hadjab, I.; Kauppinen, S.; Garon, M.; Quenneville, E.; Haapea, M.; Rieppo, L.; Pritzker, K. P.; Buschmann, M. D.; Nieminen, H. J.; Saarakkala, S.

julkaisussa: Osteoarthritis and Cartilage, Vuosikerta 26, Nro 8, 08.2018, s. 1118-1126.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography

AU - Kestilä, I.

AU - Thevenot, J.

AU - Finnilä, M. A.

AU - Karhula, S. S.

AU - Hadjab, I.

AU - Kauppinen, S.

AU - Garon, M.

AU - Quenneville, E.

AU - Haapea, M.

AU - Rieppo, L.

AU - Pritzker, K. P.

AU - Buschmann, M. D.

AU - Nieminen, H. J.

AU - Saarakkala, S.

PY - 2018/8

Y1 - 2018/8

N2 - Objective: The aims of this study were: to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (mu CT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC.Method: The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop mCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semiautomatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1: 0-10%; zone 2: 10-40%; zone 3: 40-100%) and grouped by the OARSI histological grades (OARSI grades 0-1.0, n = 6; OARSI grades 3.0-3.5, n = 6).Results: After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (PConclusion: We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/ chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA). (c) 2018 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

AB - Objective: The aims of this study were: to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (mu CT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC.Method: The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop mCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semiautomatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1: 0-10%; zone 2: 10-40%; zone 3: 40-100%) and grouped by the OARSI histological grades (OARSI grades 0-1.0, n = 6; OARSI grades 3.0-3.5, n = 6).Results: After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (PConclusion: We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/ chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA). (c) 2018 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

KW - Hexamethyldisilazane

KW - Osteoarthritis

KW - Segmentation

KW - Morphology

KW - 3D analysis

KW - SCANNING ELECTRON-MICROSCOPY

KW - ADULT HUMAN KNEE

KW - 3-DIMENSIONAL MORPHOLOGY

KW - CONFOCAL MICROSCOPY

KW - PERICELLULAR MATRIX

KW - CELL

KW - OSTEOARTHRITIS

KW - HEXAMETHYLDISILAZANE

KW - MICROENVIRONMENT

KW - ORGANIZATION

KW - 217 Medical engineering

U2 - 10.1016/j.joca.2018.05.012

DO - 10.1016/j.joca.2018.05.012

M3 - Article

VL - 26

SP - 1118

EP - 1126

JO - Osteoarthritis and Cartilage

JF - Osteoarthritis and Cartilage

SN - 1063-4584

IS - 8

ER -