Intestinal adaptation in pediatric short bowel syndrome - controlled assessment of duodenal mucosa after extensive bowel resection

Galina Sanaksenaho

Tutkimustuotos: OpinnäyteVäitöskirjaArtikkelikokoelma

Abstrakti

Background. Although extensively studied in experimental models, human data on intestinal adaptation in short bowel syndrome (SBS) is very limited. Aim of the study. We studied mucosal homeostasis of the duodenum in children with intestinal failure (IF) both during parenteral nutrition (PN) and after achieving intestinal autonomy by weaning off PN in relation to controls. Patients and methods. In total, 58 patients with IF aged from 0.5 to 23 years and 43 controls matched for age and gender without intestinal pathology were included. Duodenal biopsies obtained during clinically indicated gastroscopies were collected. We evaluated duodenal disaccharidase activities, inflammation, structural mucosal hyperplasia, proliferation, apoptosis, epithelial barrier function and nutrient transport by using the glucose oxidase method, histology, immunohistochemistry, and quantitative real-time polymerase chain reaction (qPCR) for 52 genes. Dilatation of the small bowel was assessed from a contrast small bowel series. Results. Activities of maltase and sucrase were 1.6 times lower and mucosal inflammation more frequent (22% vs 3%) in patients on current PN in comparison with controls (P˂0.05 for both). Disaccharidase activities in patients who had weaned off PN were comparable with controls. In the evaluation of mucosal morphology, proliferation, and apoptosis, PN-dependent and weaned off patients showed similar results to controls. Compared to controls, SBS patients showed a statistically significant (P
Alkuperäiskielienglanti
Valvoja/neuvonantaja
  • Pakarinen, Mikko Petteri, Valvoja
JulkaisupaikkaHelsinki
Kustantaja
Painoksen ISBN978-951-51-6409-4
TilaJulkaistu - 2020
OKM-julkaisutyyppiG5 Tohtorinväitöskirja (artikkeli)

Lisätietoja

M1 - 88 s. + liitteet

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  • 3123 Naisten- ja lastentaudit

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