Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy

A. Isomäki, T. Sillat, M. Ainola, M. Liljestrom, Y. T. Konttinen, M. Hukkanen

Tutkimustuotos: Artikkeli kirjassa/raportissa/konferenssijulkaisussaKirjan luku tai artikkeliTieteellinen


Label-free imaging technologies to monitor the events associated with
early, intermediate and late adipogenic differentiation in multipotent
mesenchymal stromal cells (MSCs) offer an attractive and convenient
alternative to conventional fixative based lipid dyes such as Oil Red O
and Sudan Red, fluorescent labels such as LipidTOX, and more indirect
methods such as qRT-PCR analyses of specific adipocyte differentiation
markers such as peroxisome PPARγ and LPL. Coherent anti-Stokes
Raman scattering (CARS) microscopy of live cells is a sensitive and fast
imaging method enabling evaluation of the adipogenic differentiation
with chemical specificity. CARS microscopy is based on imaging
structures of interest by displaying the characteristic intrinsic vibrational
contrast of chemical bonds. The method is nontoxic, non-destructive, and
minimally invasive, thus presenting a promising method for longitudinal
analyses of live cells and tissues. CARS provides a coherently emitted
signal that is much stronger than the spontaneous Raman scattering.
The anti-Stokes signal is blue shifted from the incident wavelength,
thus reducing the non-vibrational background present in most biological
materials. In this chapter, we aim to provide a detailed approach on how
to induce adipogenic differentiation in MSC cultures, and present our
methods related to label-free CARS imaging of the events associated
with the adipogenesis.
OtsikkoArthritis Research
KustantajaHumana press
ISBN (painettu)1064-3745
DOI - pysyväislinkit
TilaJulkaistu - 2014
OKM-julkaisutyyppiB2 Kirjan tai muun kokoomateoksen osa


NimiMethods in Molecular Biology
ISSN (painettu)1064-3745
ISSN (elektroninen)1940-6029


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