Lignocellulose bioconversion to ethanol by a fungal single-step consolidated method tested with waste substrates and co-culture experiments

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

The Polyporales phlebioid white rot fungus Phlebia radiata is efficient in decomposing the wood main components, and in producing ethanol from lignocelluloses and waste materials. Based to these qualifications, the fungus was adopted for design of a consolidated bioprocess method to convert wood waste materials into ethanol without pretreatments. Higher ethanol yield was aimed by introducing collaborative fungal cultivations including isolates of Saccharomyces cerevisiae, other yeasts, and a brown rot fungus. Various waste lignocellulose materials such as wheat and barley straw, recycled wood-fiber based core board, recycled construction waste wood, spruce saw dust, and birch wood were applied to represent wood and non-wood waste lignocellulose of different origin, chemical content and structure. In solid-state single cultivations with the white rot fungus P. radiata, both core board and barley straw turned out as suitable substrates for the consolidated bioprocess. Up to 32.4 ± 4.5 g/L of ethanol accumulated in the solid-state core board cultivation in 30 days whereas with barley straw, 7.0 ± 0.01 g/L of ethanol was obtained. Similar concentrations of ethanol were produced in increased-volume and higher gravity bioreactor cultivations without chemical, physical or enzymatic pretreatment. In all, our consolidated method adopting a white rot fungus is a promising and economic alternative for second generation bioethanol production from waste and residual lignocelluloses.
Alkuperäiskielienglanti
LehtiAIMS Energy
Vuosikerta6
Numero5
Sivut866-879
Sivumäärä14
ISSN2333-8326
DOI - pysyväislinkit
TilaJulkaistu - 18 lokakuuta 2018
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 414 Maatalouden bioteknologia
  • 1183 Kasvibiologia, mikrobiologia, virologia

Lainaa tätä

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title = "Lignocellulose bioconversion to ethanol by a fungal single-step consolidated method tested with waste substrates and co-culture experiments",
abstract = "The Polyporales phlebioid white rot fungus Phlebia radiata is efficient in decomposing the wood main components, and in producing ethanol from lignocelluloses and waste materials. Based to these qualifications, the fungus was adopted for design of a consolidated bioprocess method to convert wood waste materials into ethanol without pretreatments. Higher ethanol yield was aimed by introducing collaborative fungal cultivations including isolates of Saccharomyces cerevisiae, other yeasts, and a brown rot fungus. Various waste lignocellulose materials such as wheat and barley straw, recycled wood-fiber based core board, recycled construction waste wood, spruce saw dust, and birch wood were applied to represent wood and non-wood waste lignocellulose of different origin, chemical content and structure. In solid-state single cultivations with the white rot fungus P. radiata, both core board and barley straw turned out as suitable substrates for the consolidated bioprocess. Up to 32.4 ± 4.5 g/L of ethanol accumulated in the solid-state core board cultivation in 30 days whereas with barley straw, 7.0 ± 0.01 g/L of ethanol was obtained. Similar concentrations of ethanol were produced in increased-volume and higher gravity bioreactor cultivations without chemical, physical or enzymatic pretreatment. In all, our consolidated method adopting a white rot fungus is a promising and economic alternative for second generation bioethanol production from waste and residual lignocelluloses.",
keywords = "414 Agricultural biotechnology, Bioethanol, Bioconversion, Fungal biotechnology, Waste biomass, 1183 Plant biology, microbiology, virology, Microbiology, Mycology, Phlebia radiata 79, consolidated bioprocess, bioethanol, white rot fungi, waste lignocellulose, bioconversion, solid state cultivation, bioreactor, BIOETHANOL PRODUCTION, ENZYMATIC-HYDROLYSIS, PRETREATMENT, PERSPECTIVES, INSIGHT, BIOMASS, STRAW",
author = "Mattila, {Hans Kristian} and Dina Kačar and Mali, {Tuulia Leena Elina} and Lundell, {Taina Kristina}",
year = "2018",
month = "10",
day = "18",
doi = "10.3934/energy.2018.5.866",
language = "English",
volume = "6",
pages = "866--879",
journal = "AIMS Energy",
issn = "2333-8326",
publisher = "AIMS Press",
number = "5",

}

Lignocellulose bioconversion to ethanol by a fungal single-step consolidated method tested with waste substrates and co-culture experiments. / Mattila, Hans Kristian; Kačar, Dina; Mali, Tuulia Leena Elina; Lundell, Taina Kristina.

julkaisussa: AIMS Energy, Vuosikerta 6, Nro 5, 18.10.2018, s. 866-879.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Lignocellulose bioconversion to ethanol by a fungal single-step consolidated method tested with waste substrates and co-culture experiments

AU - Mattila, Hans Kristian

AU - Kačar, Dina

AU - Mali, Tuulia Leena Elina

AU - Lundell, Taina Kristina

PY - 2018/10/18

Y1 - 2018/10/18

N2 - The Polyporales phlebioid white rot fungus Phlebia radiata is efficient in decomposing the wood main components, and in producing ethanol from lignocelluloses and waste materials. Based to these qualifications, the fungus was adopted for design of a consolidated bioprocess method to convert wood waste materials into ethanol without pretreatments. Higher ethanol yield was aimed by introducing collaborative fungal cultivations including isolates of Saccharomyces cerevisiae, other yeasts, and a brown rot fungus. Various waste lignocellulose materials such as wheat and barley straw, recycled wood-fiber based core board, recycled construction waste wood, spruce saw dust, and birch wood were applied to represent wood and non-wood waste lignocellulose of different origin, chemical content and structure. In solid-state single cultivations with the white rot fungus P. radiata, both core board and barley straw turned out as suitable substrates for the consolidated bioprocess. Up to 32.4 ± 4.5 g/L of ethanol accumulated in the solid-state core board cultivation in 30 days whereas with barley straw, 7.0 ± 0.01 g/L of ethanol was obtained. Similar concentrations of ethanol were produced in increased-volume and higher gravity bioreactor cultivations without chemical, physical or enzymatic pretreatment. In all, our consolidated method adopting a white rot fungus is a promising and economic alternative for second generation bioethanol production from waste and residual lignocelluloses.

AB - The Polyporales phlebioid white rot fungus Phlebia radiata is efficient in decomposing the wood main components, and in producing ethanol from lignocelluloses and waste materials. Based to these qualifications, the fungus was adopted for design of a consolidated bioprocess method to convert wood waste materials into ethanol without pretreatments. Higher ethanol yield was aimed by introducing collaborative fungal cultivations including isolates of Saccharomyces cerevisiae, other yeasts, and a brown rot fungus. Various waste lignocellulose materials such as wheat and barley straw, recycled wood-fiber based core board, recycled construction waste wood, spruce saw dust, and birch wood were applied to represent wood and non-wood waste lignocellulose of different origin, chemical content and structure. In solid-state single cultivations with the white rot fungus P. radiata, both core board and barley straw turned out as suitable substrates for the consolidated bioprocess. Up to 32.4 ± 4.5 g/L of ethanol accumulated in the solid-state core board cultivation in 30 days whereas with barley straw, 7.0 ± 0.01 g/L of ethanol was obtained. Similar concentrations of ethanol were produced in increased-volume and higher gravity bioreactor cultivations without chemical, physical or enzymatic pretreatment. In all, our consolidated method adopting a white rot fungus is a promising and economic alternative for second generation bioethanol production from waste and residual lignocelluloses.

KW - 414 Agricultural biotechnology

KW - Bioethanol

KW - Bioconversion

KW - Fungal biotechnology

KW - Waste biomass

KW - 1183 Plant biology, microbiology, virology

KW - Microbiology

KW - Mycology

KW - Phlebia radiata 79

KW - consolidated bioprocess

KW - bioethanol

KW - white rot fungi

KW - waste lignocellulose

KW - bioconversion

KW - solid state cultivation

KW - bioreactor

KW - BIOETHANOL PRODUCTION

KW - ENZYMATIC-HYDROLYSIS

KW - PRETREATMENT

KW - PERSPECTIVES

KW - INSIGHT

KW - BIOMASS

KW - STRAW

U2 - 10.3934/energy.2018.5.866

DO - 10.3934/energy.2018.5.866

M3 - Article

VL - 6

SP - 866

EP - 879

JO - AIMS Energy

JF - AIMS Energy

SN - 2333-8326

IS - 5

ER -