Lymphangiogenesis requires Ang2/Tie/PI3K signaling for VEGFR3 cell-surface expression

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Funding Information:
We thank Riitta Kauppinen, Eleonoora Wihuri, Tapio Tainola, Karri Niiranen, Tanja Laakkonen, Jarmo Koponen, Joel Noutere, Tony Helenius, Jaana Träskelin, and Hanna Seppälä for their expert technical assistance; Mariona Graupera (Josep Carreras Leukaemia Research Institute, Barcelona) for providing Pik3cafl mice; Dieter Saur (Technische Universität München) for the R26-LSL-Pik3caH1047Rmice; and Sagrario Ortega (CNIO, Madrid) for the Vegfr3-CreERT2 mice. We thank Seppo Ylä-Herttuala (A.I. Vir-tanen Institute for Molecular Sciences, Kuopio) for providing Ad vectors; Christer Betsholtz for helpful discussions and comments on the manuscript; and Veijo Salo for assistance with the image analysis. We also thank the Biomedicum Imaging Unit; the Flow Cytometry Unit at the Helsinki Institute of Life Sciences (HiLIFE) at the University of Helsinki; and the Laboratory Animal Centers at HiLIFE; the University of Helsinki and University of Oulu; and the Transgenic and Tissue Phenotyping Core Facility at Biocenter Oulu, supported by the University of Oulu and Biocenter Finland and the Single cell core Facility at Flemingsberg campus (SICOF) at the Karolinska Institute for providing professional services and valuable help. The illustrative graphs in Figure 8A and Supplemental Figure 14A were created using BioRender. This work was funded by the Wihuri Foundation (to KA, EAK, AM, SKJ, AA, VML, IS, PS, and KV); the European Union’s Horizon 2020 Research and Innovation Programme under grant agreements 874708 (THER-ALYMPH, to KA) and 773076 (to PS); the Novo Nordisk Foundation (grant NNF16OC0023554, to KA); the Sigrid Jusélius Foundation (to KA, PS, KV, and LE); the Hospital District of Helsinki and the Uusimaa Research Grant (to KA); the Cancer Foundation Finland (to KA and PS); the Academy of Finland Centre of Excellence Program (307366, to KA); the Academy of Finland Research Costs of Academy Professor (312516, to KA); the Maud Kuistila Memorial Foundation (to EAK); the Ida Montini Foundation (to EAK); The Priority Academic Program Development of Jiangsu Higher Education Institutions (to YH); the National Natural Science Foundation of China (31970768, to YH); the Knut and Alice Wallenberg Foundation (2018.0218, to TM); the Swedish Research Council (2020-02692, to TM); the Academy of Finland (310986, to LE; 310075, to PS); the Academy of Finland Academy Fellow Funding (315710, to KV); and the European Research Council Advanced Grant Angiomature (project 787181, to HGA).

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