Manganese peroxidases, laccases and oxalic acid from the selective white-rot fungus Physisporinus rivulosus grown on spruce wood chips

Terhi Hakala, Taina Lundell, Sari Galkin, Pekka Maijala, Nisse Kalkkinen, Annele Hatakka

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Abstrakti

The white-rot fungus Physisporinus rivulosus T241i produced manganese peroxidase (MnP), laccase, and oxalic acid when it was grown on spruce (Picea abies) wood chips. This white-rot basidiomycete degrades lignin selectively and is promising for use in biopulping of softwood. During solid-state cultivation on wood chips the fungus produced oxalic acid (28 mumol/g dry wood) and a high MnP activity (24 nkat/g dry wood), while measured laccase activity was negligible. The prepurified laccase and MnP isoforms were fractionated with anion exchange chromatography. Characterization of the isolated MnP fractions indicated the presence of several MnP isoforms with pI values between 3.4 and 3.9 and molecular masses between 47 and 52 kDa. The four laccase isoforms had pI values between 3.1 and 3.3 and molecular masses between 66 and 68 kDa. The MnP and laccase isoform profiles did not vary during the 4-week cultivation. The N-terminal amino acid sequences of four laccase and two MnP isoenzymes resembled those of the two other selective white-rot fungi applied in biopulping, i.e. Ceriporiopsis subvermispora CZ-3 and the strain IZU-154. (C) 2004 Elsevier Inc. All rights reserved.
Alkuperäiskielienglanti
LehtiEnzyme and microbial technology
Vuosikerta36
Numero4
Sivut461-468
Sivumäärä8
ISSN0141-0229
DOI - pysyväislinkit
TilaJulkaistu - 2005
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

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