Manipulation of developmental function in turtles with notes on alligators

Jacqueline Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul Kulesa, Judith A. Cebra-Thomas

Tutkimustuotos: Artikkeli kirjassa/raportissa/konferenssijulkaisussaKirjan luku tai artikkeliTieteellinen

Abstrakti

Reptiles have great taxonomic diversity that is reflected in their morphology, ecology, physiology, modes of reproduction, and development. Interest in comparative and evolutionary developmental biology makes protocols for the study of reptile embryos invaluable resources. The relatively large size, seasonal breeding, and long gestation times of turtles epitomize the challenges faced by the developmental biologist. We describe protocols for the preparation of turtle embryos for ex ovo culture, electroporation, in situ hybridization, and micro-computed tomography. Because these protocols have been adapted and optimized from methods used for frog, chick, and mouse embryos, it is likely that they could be used for other reptilian species. Notes are included for alligator embryos where appropriate.
Alkuperäiskielienglanti
OtsikkoVertebrate Embryogenesis : Embryological, Cellular and Genetic Methods
Sivumäärä17
KustantajaSpringer
Julkaisupäivähelmikuuta 2019
Sivut247-263
ISBN (painettu)978-1-61779-209-0
ISBN (elektroninen)978-1-61779-210-6
DOI - pysyväislinkit
TilaJulkaistu - helmikuuta 2019
OKM-julkaisutyyppiB2 Kirjan tai muun kokoomateoksen osa

Julkaisusarja

NimiMethods in Molecular Biology
KustantajaHumana Press
ISSN (elektroninen)1064-3745

Tieteenalat

  • 1182 Biokemia, solu- ja molekyylibiologia

Siteeraa tätä