Means to Quantify Vascular Cell File Numbers in Different Tissues

Helena E. Arents, Gugan Eswaran, Matouš Glanc, Ari Pekka Mähönen, Bert De Rybel

Tutkimustuotos: Artikkeli kirjassa/raportissa/konferenssijulkaisussaKirjan luku tai artikkeliTieteellinenvertaisarvioitu

Abstrakti

Oriented cell divisions are crucial throughout plant development to define the final size and shape of organs and tissues. As most of the tissues in mature roots and stems are derived from vascular tissues, studying cell proliferation in the vascular cell lineage is of great importance. Although perturbations of vascular development are often visible already at the whole plant macroscopic phenotype level, a more detailed characterization of the vascular anatomy, cellular organization, and differentiation status of specific vascular cell types can provide insights into which pathway or developmental program is affected. In particular, defects in the frequency or orientation of cell divisions can be reliably identified from the number of vascular cell files. Here, we provide a detailed description of the different clearing, staining, and imaging techniques that allow precise phenotypic analysis of vascular tissues in different organs of the model plant Arabidopsis thaliana throughout development, including the quantification of cell file numbers, differentiation status of vascular cell types, and expression of reporter genes.

Alkuperäiskielienglanti
OtsikkoPlant Cell Division : Methods and Protocols
ToimittajatMarie-Cécile Caillaud
Sivumäärä25
JulkaisupaikkaNew York
KustantajaHumana press
Julkaisupäivä2022
Sivut155-179
ISBN (painettu)978-1-0716-1743-4, 978-1-0716-1746-5
ISBN (elektroninen)978-1-0716-1744-1
DOI - pysyväislinkit
TilaJulkaistu - 2022
OKM-julkaisutyyppiA3 Kirjan tai muun kokoomateoksen osa

Julkaisusarja

NimiMethods in Molecular Biology
Vuosikerta2382
ISSN (painettu)1064-3745
ISSN (elektroninen)1940-6029

Lisätietoja

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Tieteenalat

  • 11831 Kasvibiologia

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