Minor spliceosome components are predominantly localized in the nucleus

Heli K. J Pessa, Cindy L Will, Xiaojuan Meng, Claudia Schneider, Nicholas J Watkins, Nina Perälä, Mariann Nymark, Janne J Turunen, Reinhard Lührmann, Mikko J Frilander

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Recently, it has been reported that there is a differential subcellular distribution of components of the minor U12-dependent and major U2-dependent spliceosome, and further that the minor spliceosome functions in the cytoplasm. To study the subcellular localization of the snRNA components of both the major and minor spliceosomes, we performed in situ hybridizations with mouse tissues and human cells. In both cases, all spliceosomal snRNAs were nearly exclusively detected in the nucleus, and the minor U11 and U12 snRNAs were further shown to colocalize with U4 and U2, respectively, in human cells. Additionally, we examined the distribution of several spliceosomal snRNAs and proteins in nuclear and cytoplasmic fractions isolated from human cells. These studies revealed an identical subcellular distribution of components of both the U12- and U2-dependent spliceosomes. Thus, our data, combined with several earlier publications, establish that, like the major spliceosome, components of the U12-dependent spliceosome are localized predominantly in the nucleus.
Alkuperäiskielienglanti
LehtiProceedings of the National Academy of Sciences of the United States of America
Vuosikerta105
Numero25
Sivut8655-8660
Sivumäärä6
ISSN0027-8424
DOI - pysyväislinkit
TilaJulkaistu - 2008
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Lainaa tätä

Pessa, Heli K. J ; Will, Cindy L ; Meng, Xiaojuan ; Schneider, Claudia ; Watkins, Nicholas J ; Perälä, Nina ; Nymark, Mariann ; Turunen, Janne J ; Lührmann, Reinhard ; Frilander, Mikko J. / Minor spliceosome components are predominantly localized in the nucleus. Julkaisussa: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vuosikerta 105, Nro 25. Sivut 8655-8660.
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title = "Minor spliceosome components are predominantly localized in the nucleus",
abstract = "Recently, it has been reported that there is a differential subcellular distribution of components of the minor U12-dependent and major U2-dependent spliceosome, and further that the minor spliceosome functions in the cytoplasm. To study the subcellular localization of the snRNA components of both the major and minor spliceosomes, we performed in situ hybridizations with mouse tissues and human cells. In both cases, all spliceosomal snRNAs were nearly exclusively detected in the nucleus, and the minor U11 and U12 snRNAs were further shown to colocalize with U4 and U2, respectively, in human cells. Additionally, we examined the distribution of several spliceosomal snRNAs and proteins in nuclear and cytoplasmic fractions isolated from human cells. These studies revealed an identical subcellular distribution of components of both the U12- and U2-dependent spliceosomes. Thus, our data, combined with several earlier publications, establish that, like the major spliceosome, components of the U12-dependent spliceosome are localized predominantly in the nucleus.",
author = "Pessa, {Heli K. J} and Will, {Cindy L} and Xiaojuan Meng and Claudia Schneider and Watkins, {Nicholas J} and Nina Per{\"a}l{\"a} and Mariann Nymark and Turunen, {Janne J} and Reinhard L{\"u}hrmann and Frilander, {Mikko J}",
year = "2008",
doi = "10.1073/pnas.0803646105",
language = "English",
volume = "105",
pages = "8655--8660",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "25",

}

Minor spliceosome components are predominantly localized in the nucleus. / Pessa, Heli K. J; Will, Cindy L; Meng, Xiaojuan; Schneider, Claudia; Watkins, Nicholas J; Perälä, Nina; Nymark, Mariann; Turunen, Janne J; Lührmann, Reinhard; Frilander, Mikko J.

julkaisussa: Proceedings of the National Academy of Sciences of the United States of America, Vuosikerta 105, Nro 25, 2008, s. 8655-8660.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Minor spliceosome components are predominantly localized in the nucleus

AU - Pessa, Heli K. J

AU - Will, Cindy L

AU - Meng, Xiaojuan

AU - Schneider, Claudia

AU - Watkins, Nicholas J

AU - Perälä, Nina

AU - Nymark, Mariann

AU - Turunen, Janne J

AU - Lührmann, Reinhard

AU - Frilander, Mikko J

PY - 2008

Y1 - 2008

N2 - Recently, it has been reported that there is a differential subcellular distribution of components of the minor U12-dependent and major U2-dependent spliceosome, and further that the minor spliceosome functions in the cytoplasm. To study the subcellular localization of the snRNA components of both the major and minor spliceosomes, we performed in situ hybridizations with mouse tissues and human cells. In both cases, all spliceosomal snRNAs were nearly exclusively detected in the nucleus, and the minor U11 and U12 snRNAs were further shown to colocalize with U4 and U2, respectively, in human cells. Additionally, we examined the distribution of several spliceosomal snRNAs and proteins in nuclear and cytoplasmic fractions isolated from human cells. These studies revealed an identical subcellular distribution of components of both the U12- and U2-dependent spliceosomes. Thus, our data, combined with several earlier publications, establish that, like the major spliceosome, components of the U12-dependent spliceosome are localized predominantly in the nucleus.

AB - Recently, it has been reported that there is a differential subcellular distribution of components of the minor U12-dependent and major U2-dependent spliceosome, and further that the minor spliceosome functions in the cytoplasm. To study the subcellular localization of the snRNA components of both the major and minor spliceosomes, we performed in situ hybridizations with mouse tissues and human cells. In both cases, all spliceosomal snRNAs were nearly exclusively detected in the nucleus, and the minor U11 and U12 snRNAs were further shown to colocalize with U4 and U2, respectively, in human cells. Additionally, we examined the distribution of several spliceosomal snRNAs and proteins in nuclear and cytoplasmic fractions isolated from human cells. These studies revealed an identical subcellular distribution of components of both the U12- and U2-dependent spliceosomes. Thus, our data, combined with several earlier publications, establish that, like the major spliceosome, components of the U12-dependent spliceosome are localized predominantly in the nucleus.

U2 - 10.1073/pnas.0803646105

DO - 10.1073/pnas.0803646105

M3 - Article

VL - 105

SP - 8655

EP - 8660

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 25

ER -