Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion

Ann-Katrin Llarena, Ji Zhang, Minna Vehkala, Niko Välimäki, Marjaana Hakkinen, Marja-Liisa Hänninen, Mati Roasto, Mihkel Mäesaar , Eduardo Taboada, Dillon Barker, Giuliano Garofolo, Cesare Cammà, Elisabetta Di Giannatale, Jukka Corander, Mirko Rossi

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

The decreased costs of genome sequencing have increased the capability to apply whole-genome sequencing to epidemiological surveillance of zoonotic Campylobacter jejuni. However, knowledge of the genetic diversity of this bacteria is vital for inferring relatedness between epidemiologically linked isolates and a necessary prerequisite for correct application of this methodology. To address this issue in C. jejuni we investigated the spatial and temporal signals in the genomes of a major clonal complex and generalist lineage, ST-45 CC, by analysing the population structure and genealogy as well as applying genome-wide association analysis of 340 isolates from across Europe collected over a wide time range. The occurrence and strength of the geographical signal varied between sublineages and followed the clonal frame when present, while no evidence of a temporal signal was found. Certain sublineages of ST-45 formed discrete and genetically isolated clades containing isolates with extremely similar genomes regardless of time and location of sampling. Based on a separate data set, these monomorphic genotypes represent successful C. jejuni clones, possibly spread around the globe by rapid animal (migrating birds), food or human movement. In addition, we observed an incongruence between the genealogy of the strains and multilocus sequence typing (MLST), challenging the existing clonal complex definition and the use of whole-genome gene-by-gene hierarchical nomenclature schemes for C. jejuni.
Alkuperäiskielienglanti
LehtiMicrobial Genomics
Vuosikerta2
Sivumäärä14
ISSN2057-5858
DOI - pysyväislinkit
TilaJulkaistu - 1 lokakuuta 2016
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1183 Kasvibiologia, mikrobiologia, virologia

Lainaa tätä

Llarena, Ann-Katrin ; Zhang, Ji ; Vehkala, Minna ; Välimäki, Niko ; Hakkinen, Marjaana ; Hänninen, Marja-Liisa ; Roasto, Mati ; Mäesaar , Mihkel ; Taboada, Eduardo ; Barker, Dillon ; Garofolo, Giuliano ; Cammà, Cesare ; Di Giannatale, Elisabetta ; Corander, Jukka ; Rossi, Mirko. / Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion. Julkaisussa: Microbial Genomics. 2016 ; Vuosikerta 2.
@article{d0b0fc541a724fd99852d23a238b79fd,
title = "Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion",
abstract = "The decreased costs of genome sequencing have increased the capability to apply whole-genome sequencing to epidemiological surveillance of zoonotic Campylobacter jejuni. However, knowledge of the genetic diversity of this bacteria is vital for inferring relatedness between epidemiologically linked isolates and a necessary prerequisite for correct application of this methodology. To address this issue in C. jejuni we investigated the spatial and temporal signals in the genomes of a major clonal complex and generalist lineage, ST-45 CC, by analysing the population structure and genealogy as well as applying genome-wide association analysis of 340 isolates from across Europe collected over a wide time range. The occurrence and strength of the geographical signal varied between sublineages and followed the clonal frame when present, while no evidence of a temporal signal was found. Certain sublineages of ST-45 formed discrete and genetically isolated clades containing isolates with extremely similar genomes regardless of time and location of sampling. Based on a separate data set, these monomorphic genotypes represent successful C. jejuni clones, possibly spread around the globe by rapid animal (migrating birds), food or human movement. In addition, we observed an incongruence between the genealogy of the strains and multilocus sequence typing (MLST), challenging the existing clonal complex definition and the use of whole-genome gene-by-gene hierarchical nomenclature schemes for C. jejuni.",
keywords = "1183 Plant biology, microbiology, virology",
author = "Ann-Katrin Llarena and Ji Zhang and Minna Vehkala and Niko V{\"a}lim{\"a}ki and Marjaana Hakkinen and Marja-Liisa H{\"a}nninen and Mati Roasto and Mihkel M{\"a}esaar and Eduardo Taboada and Dillon Barker and Giuliano Garofolo and Cesare Camm{\`a} and {Di Giannatale}, Elisabetta and Jukka Corander and Mirko Rossi",
year = "2016",
month = "10",
day = "1",
doi = "10.1099/mgen.0.000088",
language = "English",
volume = "2",
journal = "Microbial Genomics",
issn = "2057-5858",
publisher = "American Society for Microbiology",

}

Llarena, A-K, Zhang, J, Vehkala, M, Välimäki, N, Hakkinen, M, Hänninen, M-L, Roasto, M, Mäesaar , M, Taboada, E, Barker, D, Garofolo, G, Cammà, C, Di Giannatale, E, Corander, J & Rossi, M 2016, 'Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion', Microbial Genomics, Vuosikerta 2. https://doi.org/10.1099/mgen.0.000088

Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion. / Llarena, Ann-Katrin; Zhang, Ji; Vehkala, Minna; Välimäki, Niko; Hakkinen, Marjaana; Hänninen, Marja-Liisa; Roasto, Mati; Mäesaar , Mihkel ; Taboada, Eduardo; Barker, Dillon; Garofolo, Giuliano ; Cammà, Cesare ; Di Giannatale, Elisabetta ; Corander, Jukka; Rossi, Mirko.

julkaisussa: Microbial Genomics, Vuosikerta 2, 01.10.2016.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion

AU - Llarena, Ann-Katrin

AU - Zhang, Ji

AU - Vehkala, Minna

AU - Välimäki, Niko

AU - Hakkinen, Marjaana

AU - Hänninen, Marja-Liisa

AU - Roasto, Mati

AU - Mäesaar , Mihkel

AU - Taboada, Eduardo

AU - Barker, Dillon

AU - Garofolo, Giuliano

AU - Cammà, Cesare

AU - Di Giannatale, Elisabetta

AU - Corander, Jukka

AU - Rossi, Mirko

PY - 2016/10/1

Y1 - 2016/10/1

N2 - The decreased costs of genome sequencing have increased the capability to apply whole-genome sequencing to epidemiological surveillance of zoonotic Campylobacter jejuni. However, knowledge of the genetic diversity of this bacteria is vital for inferring relatedness between epidemiologically linked isolates and a necessary prerequisite for correct application of this methodology. To address this issue in C. jejuni we investigated the spatial and temporal signals in the genomes of a major clonal complex and generalist lineage, ST-45 CC, by analysing the population structure and genealogy as well as applying genome-wide association analysis of 340 isolates from across Europe collected over a wide time range. The occurrence and strength of the geographical signal varied between sublineages and followed the clonal frame when present, while no evidence of a temporal signal was found. Certain sublineages of ST-45 formed discrete and genetically isolated clades containing isolates with extremely similar genomes regardless of time and location of sampling. Based on a separate data set, these monomorphic genotypes represent successful C. jejuni clones, possibly spread around the globe by rapid animal (migrating birds), food or human movement. In addition, we observed an incongruence between the genealogy of the strains and multilocus sequence typing (MLST), challenging the existing clonal complex definition and the use of whole-genome gene-by-gene hierarchical nomenclature schemes for C. jejuni.

AB - The decreased costs of genome sequencing have increased the capability to apply whole-genome sequencing to epidemiological surveillance of zoonotic Campylobacter jejuni. However, knowledge of the genetic diversity of this bacteria is vital for inferring relatedness between epidemiologically linked isolates and a necessary prerequisite for correct application of this methodology. To address this issue in C. jejuni we investigated the spatial and temporal signals in the genomes of a major clonal complex and generalist lineage, ST-45 CC, by analysing the population structure and genealogy as well as applying genome-wide association analysis of 340 isolates from across Europe collected over a wide time range. The occurrence and strength of the geographical signal varied between sublineages and followed the clonal frame when present, while no evidence of a temporal signal was found. Certain sublineages of ST-45 formed discrete and genetically isolated clades containing isolates with extremely similar genomes regardless of time and location of sampling. Based on a separate data set, these monomorphic genotypes represent successful C. jejuni clones, possibly spread around the globe by rapid animal (migrating birds), food or human movement. In addition, we observed an incongruence between the genealogy of the strains and multilocus sequence typing (MLST), challenging the existing clonal complex definition and the use of whole-genome gene-by-gene hierarchical nomenclature schemes for C. jejuni.

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1099/mgen.0.000088

DO - 10.1099/mgen.0.000088

M3 - Article

VL - 2

JO - Microbial Genomics

JF - Microbial Genomics

SN - 2057-5858

ER -