Novel anti-infective potential of Salvianolic acid B against human serious pathogen Neisseria meningitidis

Julkaisun otsikon käännös: Salvianolihappo-B- potentiaalinen anti-infektiivi Neisseria meningitidia-bakteeri-infektioita vastaan : SA-B meninkokki-infektioita vastaan

Sanna Huttunen, Marko Toivanen, Cheng-Hai Liu, Carina Alexandra Tikkanen-Kaukanen

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Abstrakti

New methods against bacterial infections are needed due to antibiotic resistance and lack of effective vaccines. Antiadhesion therapy is a modern approach in preventing bacterial attachment to host cells. In our previous studies we have shown that berry polyphenolic fractions have binding inhibition activity against serious human pathogen Neisseria meningitidis. In this study new binding inhibitor against N. meningitidis was searched from a Chinese herbal medicine, Salvianolic acid B (SA-B), which is an active polyphenol isolated from the radix Salviae miltiorrhizae. Inhibition of meningococcal pili binding to bovine thyroglobulin, (reference glycoprotein for meningococcal binding), and to human epithelial cells (HEC-1B) was tested. SA-B inhibited pili binding to bovine thyroglobulin totally at the concentration of 0.3 mg/mL, and to HEC-1B cells at the concentration of 0.5 mg/mL. 50 % binding inhibition activity to bovine thyroglobulin was achieved with 0.6 µg/mL. Antimicrobial activity of SA-B against N. meningitidis was found only in relatively high concentrations. For comparison polyphenol macromolecular complexes isolated from Fructus Momordica (Momordica grosvenori Swingle) and wolfberry (Lycium barbarum L.) had only slight or none binding inhibitory effects. Our results indicate that SA-B may have potential in preventing infections by inhibiting meningococcal binding to host cells.

New methods against bacterial infections are needed due to antibiotic resistance and lack of effective vaccines. Antiadhesion therapy is a modern approach in preventing bacterial attachment to host cells. In our previous studies we have shown that berry polyphenolic fractions have binding inhibition activity against serious human pathogen Neisseria meningitidis. In this study new binding inhibitor against N. meningitidis was searched from a Chinese herbal medicine, Salvianolic acid B (SA-B), which is an active polyphenol isolated from the radix Salviae miltiorrhizae. Inhibition of meningococcal pili binding to bovine thyroglobulin, (reference glycoprotein for meningococcal binding), and to human epithelial cells (HEC-1B) was tested. SA-B inhibited pili binding to bovine thyroglobulin totally at the concentration of 0.3 mg/mL, and to HEC-1B cells at the concentration of 0.5 mg/mL. 50 % binding inhibition activity to bovine thyroglobulin was achieved with 0.6 µg/mL. Antimicrobial activity of SA-B against N. meningitidis was found only in relatively high concentrations. For comparison polyphenol macromolecular complexes isolated from Fructus Momordica (Momordica grosvenori Swingle) and wolfberry (Lycium barbarum L.) had only slight or none binding inhibitory effects. Our results indicate that SA-B may have potential in preventing infections by inhibiting meningococcal binding to host cells.

Julkaisun otsikon käännösSalvianolihappo-B- potentiaalinen anti-infektiivi Neisseria meningitidia-bakteeri-infektioita vastaan : SA-B meninkokki-infektioita vastaan
Alkuperäiskielienglanti
LehtiBMC research notes
Vuosikerta9
Numero25
Sivumäärä6
ISSN1756-0500
DOI - pysyväislinkit
TilaJulkaistu - 13 tammikuuta 2016
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 317 Farmasia
  • antiadhesion therapy,
  • antimicrobial
  • salvianolic acid B
  • 416 Elintarviketieteet
  • polyphenol
  • functional foods
  • Salviae miltiorrhizae
  • 3142 Kansanterveystiede, ympäristö ja työterveys
  • Neisseria meningitidis
  • MENINGITIS

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